Bio-Hacking meets Citizen Science

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DOCUMENTATION

See biodesign.cc and more about the PCR your DNA Workshop.

WHEN

NEW DATE
Thursday 28th January 2016

Postponed....

9:45 -16:00 November 20th 2015

WHERE

House of the Academies, Laupenstrasse 7, Bern
Room „Mönch“, ground floor

http://www.science-et-cite.ch/

Sign Up

The number of participants for the workshop is limited. Please sign up by sending an email to cs (at) science-et-cite.ch by January 15 if you would like to take part in the workshop, or in the afternoon session, or both.

Costs

Costs: There is such a thing as a free lunch (maybe not at Wallstreet, but at the Haus der Akademien)

Schedule:

NEW Program

  1. a hands-on workshop in the morning and early afternoon
  2. a network meeting (network news, 3 short talks on biohacking and a long discussion) in the afternoon.


Workshop and talks are held by three people from the hackteria community (http://hackteria.org/):


Program
May change during the day. not updated version. see pdf.

9:30 Arrival of Participants, Welcome
9:45 Introduction and news from the Swiss Citizen Science Network
Pia Viviani, Jenny Flück

10:00 Workshop: a biohacker's kitchen laboratory
Urs Gaudenz, Sachiko Hirosue, Marc Dusseiller
- DIY experiments in the kitchen laboratory
- Showcase: biohacking tools for citizen science (various prototypes)

12:00 Lunch break and informal discussions

13:00 Overview: global transdisciplinary approaches to participatory science
Dr. Marc Dusseiller, transdisciplinary educator, biohacker and Hackteria's Global Ambassador
Focus: international, transdisciplinary education (mostly about coconuts)

13:20 Science hacking, open source scientific lab equipment
Dipl. Ing. Urs Gaudenz, lecturer for open innovation (HSLU) and open hardware developer (GaudiLabs)
Focus: true collaboration between institutional and non-institutional

13:40 Case study: bio-design for the real world - a collaborative model for interdisciplinary student education
Dr. Sachiko Hirosue, senior scientist EPFL, initiator of biodesign.cc
Focus: real world application, student involvement

14:00 Discussion – the role and responsibility of biohacking in citizen science
Moderator: Dipl. Ing. Urs Gaudenz

16:00 End

Impressions


Slides

Workshop

Collecting genetic information for citizen science
Citizen science is not something new that arrived with DIYbiology or the era of crowd-sourcing. It has deep roots in formal societies, activist groups and other informal collectives of people that observe and documenting wildlife, natural resources, uncovering pollution, to name a few.
With the DIY OpenPCR, and other simple equipment available in the generic lab equipment, what could be a citizen science project that could be molecular?
There are already projects out there.

  • Armpit Life - where 2 day unwashed armpits are swabbed for culture and microbiome (deep sequencing) analysis (US)
  • Crowd-sourcing Taste Research - Denver Museum of Nature & Science (Museum) asks museum visitors for samples, the link is a scientific manuscript!! (US)
  • Beer Decoded - find out the genetic imprint of your favorite beer. (CH)


We will be taking samples from the workshop participants (with their consent), and throughout the day, we will try to PCR out their genotypes of the dopamine 4 receptor and the serotonin transporter, and observing the differences amongst us.
These types of activities bring forth several questions of genetic privacy, who owns the data, open questions of genetics on our lives - how should these results be interpreted?

Detailed Information on the Workshop

We have now extensively documented the steps of the DNA analysis on the PCR your DNA Workshop wiki page, and the primer information is on the BIODESIGN for the REAL WORLD entry on the Workshop.

References

http://www.bag.admin.ch/themen/medizin/00683/02724/03676/index.html?lang=de

Material List

Urs brings:
- Laser cut parts for gel carriers (12x)
- Combs for gel (24x)
- 2x GelBox
- Blue Transilluminator
- Silicon glue
- Agar agar
- 1x High Power Supply (for Gel)
- HotPlate
- Flask for coocking agar
- Tesa Film to cast gel carriers
- Food colors for easy gel run
- Salt for gel buffer
- MyPCR thermocycler
- MicroTubes for PCR
- Sand paper
- Power Cords
- Banana Clips
- 9V Batteries
- Pipettor, Tips (?)
-
- Sleeping Bag

Marc brings:
- Tupper ware for DIY GelBox
- Glue for Acrylic (Urs has no more)
- Coconut & stuff for demo
-


Sachiko brings:
- PCR primers
- PCR mix
- agarose
- 100bp ladder
- PCR water
- clean tips and pipettes
- gel red - SYBR safe
- PCR tubes
- Open PCR
- powerbox
- PCR tube rack - fresh Q tips!!

Readings

Synthetische Biologie - die nächste Stufe der Bio- und Gentechnologie

TAB synbio egolabs.jpg

Zeitgleich mit dem TAB-Arbeitsbericht Nr. 164 zum Thema »Synthetische Biologie« erscheint der neue TAB-Brief, dessen Schwerpunkt ebenfalls diese »nächste Stufe der Bio- und Gentechnologie« behandelt. Die Bearbeitung dieses durchaus sperrigen, weil unübersichtlichen Themenfeldes stellte eine echte Herausforderung dar, konnte aber durch eine intensive Zusammenarbeit mit Kollegen aus dem ITAS des KIT sowie die vielfältige Einbindung externer Expertise erfolgreich zu Ende geführt werden.

http://www.tab-beim-bundestag.de/de/aktuelles/20151221.html

IN KÜRZE Wissenschaft liche und technologische Fortschritte ermöglichen einen zunehmend tiefer greifenden genetischen Umbau natürlicher Organismen (Synbio i. w. S.), perspektivisch wird die Schaff ung künstlicher biologischer Systeme (Synbio i. e. S.) anvisiert. Anwendungsbereiche liegen in der Chemie- und Energieproduktion, in der Medizin und im Umweltschutz. Welche Ansätze der Synbio sich durchsetzen werden gegenüber Verfahren, die die vorhandene biologische Vielfalt nutzen oder sich auf subtile Eingriff e beschrän- ken, ist beim heutigen Stand von Forschung und Entwicklung nicht verlässlich prognostizierbar. Für die Risikoabschätzung und -bewertung substanziell veränderter Organismen müssen neue Methoden und Verfahren entwickelt und erprobt werden. Die Partizipationsmöglichkeiten der Gesellschaft an einer verantwortungsvollen Weiterentwicklung der Synbio reichen von einer Stakeholderbeteiligung an der Forschungsprogrammatik bis zur DIY-Biologie. Der Umgang mit geistigem Eigentum unter den Be- dingungen einer zunehmend digitalen Ökonomie stellt eine der zentralen Zukunft sherausforderungen auch für die Nutzung der Synbio dar.

Positionspapier: Biohacking als emanzipierte Citizen Science

Selection 818.png

Rüdiger Trojok (Biotinkering) in Kooperation mit ITAS/KIT

File:Eingabe GEWISS_rüdiger.pdf