http://www.hackteria.org/wiki/api.php?action=feedcontributions&user=Derishus&feedformat=atomHackteria Wiki - User contributions [en]2024-03-29T10:32:50ZUser contributionsMediaWiki 1.28.0http://www.hackteria.org/wiki/index.php?title=WetWare&diff=33577WetWare2019-08-19T15:51:44Z<p>Derishus: /* Molecular Biology */</p>
<hr />
<div>WetWare Shopping<br><br />
For independent scientists or artists doing DIYBio it is not always easy to get the biomaterials and wet ware easily. Here is a list of shops that are hacker-friendly and cheap:<br><br />
If you know a good store that you can recommend, please add it to the list.<br><br />
<br><br />
<br><br />
<blockquote>"If you start to order reagents yourself, start by ordering something innocuous. This will allow you to be a registered customer without raising red flags. Then graduate to ordering the harder stuff. "[https://medium.com/@Sachiko/lab-making-d573afa93231 Lab Making]</blockquote><br />
<br><br />
<br><br />
[[File:WetWare_s.jpg|600px]]<br />
==Chemistry==<br />
<br><br />
Chemistry, good quality, cheap and big choice from ebay:<br><br />
http://stores.ebay.de/s3chemicals/<br><br />
<br><br />
<br />
<br><br />
Poll on FaceBook by Alex Kelly:<br><br />
"Sunday morning question: What basic equipment/reagent/material have you struggled to obtain on the open consumer market?":<br><br />
<br><br />
* Sucrose and amino acids from sigma, "sent to US residential adress for a legit company - but they insisted on an official lab"<br><br />
* Chloral hydrate<br><br />
* Beakers<br><br />
* Methylamine<br><br />
* Love<br><br />
* Temperature regulators / thermostats. Julabo or Huber. "No second hand units available in Australia"<br><br />
* pure ethanol -"believe it or not".<br><br />
* Dichloromethane<br><br />
* The common inorganic acids (e.g. conc H2SO4)<br><br />
* Lithium acetate<br><br />
* Methacrylic anhydride<br><br />
* PEG in different molecular weights, PEG in 8000 and 3350<br><br />
* Glacial HOAc<br><br />
* Acetone AR<br><br />
* 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Is a photoinitiator in case you're wondering.)<br><br />
* NaOH solid (prills/pellets/whatever) (Must be AR, if I wanted Bunnings-grade I'd just buy it.)<br><br />
* A lot of the acetate salts (and acid) tend to pop up a lot in molecular biology and biochemistry in buffers etc.<br><br />
* HCl<br><br />
* H2SO4<br><br />
* Etehanol<br><br />
* CaCl2<br><br />
* MgCl2<br><br />
* Cost-effective DNA dyes such as ethidium / GelRed / SYBR / whatever<br><br />
* EDTA disodium dihydrate (sometimes people specify EDTA without specifying which salt and which hydrate, which is a pain because you don't specifically know what the counter ion is, or the molecular weight.)<br><br />
* Glycerol, DMSO, MgCl2, CaCl2, EDTA, Tris<br><br />
* Glycerol and DMSO<br><br />
* Pure NaCl<br><br />
* Agarose<br><br />
* Agar<br><br />
* Common media such as LB as well as individual components such as yeast extract and peptone etc.<br><br />
* Premix common buffers such as TAE<br><br />
* Common antibiotics e.g. ampicillin<br><br />
* Dextrose<br><br />
* L-Arabinose<br><br />
* Organic solvents that don't leave residue when evaporated<br><br />
* Ion-exchange resins for DNA workup, like Chelex sort of stuff.<br><br />
* Chloroform and phenol.<br><br />
* Isoamyl alcohol<br><br />
* PCR Mastermix<br><br />
* petridish<br><br />
* Schott bottles (or clones) in a spectrum of sizes including the little 100ml ones.<br><br />
* Stock microorganisms. Nothing dangerous but the common risk group 1 stuff. Like stock of Saccharomyces and K12.<br><br />
* strains of mice<br><br />
* Stock plasmids (e.g. common expression vectors, scaffolds, Cas9 templates)<br><br />
* monopotassium phosphate and dipotassium phosphate<br><br />
<br />
<br><br />
<br><br />
Thanks to everyone who helped compile this list...<br><br />
"Perhaps some of us should start a business selling scientific supplies, reagents etc marketed towards citizen scientist, hackers etc.. Could sell cheap kits for open source hardware too".. (Leo Mason)<br><br />
<br><br />
<br />
[http://www.sciencebuddies.org/science-fair-projects/project_ideas/Chem_Purchasing.shtml Chemical Purchasing] list on sciencebuddies.org.<br />
<br />
==Molecular Biology==<br />
The ODIN - The Open Discovery Institute - ebe:<br><br />
http://www.the-odin.com/<br><br />
<br><br />
Good quality TAQ and home brewing of biomaterials:<br><br />
https://www.geneandcell.com/<br><br />
<br><br />
Agarose and Tubes from ebay:<br><br />
http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48<br><br />
<br><br />
Petri Dishes, pipette tips, PCR Tubes and other stuff. (No small quantities. Price is OK, but not really cheap)<br><br />
Sarstedt, Switzerland:<br><br />
https://www.sarstedt.com/home/<br><br />
<br><br />
<br><br />
Laboratory Equipment, Life science, chemicals. (Not really cheap)<br><br />
Carl Roth, Switzerland<br><br />
https://www.carlroth.com/ch/de/<br><br />
<br><br />
<br><br />
Biohacker.jp articles (in Japanese) on [[http://biohacker.jp/c/BH230.html?fbclid=IwAR1xgRbhUHZUQg1BvWf76L9Cvz460dlBM5PWpjFWcDxtMYJC4fwfvxwYGts where to buy reagents]].<br />
<br><br />
<br><br />
<br />
==Plant Biology==<br />
[http://www.plantmedia.com/ Plant Media.com]<br />
[[Category:Wetware]]<br />
[[Category:Chemistry]]<br />
[[Category:Materials]]<br />
[[Category:Molecular]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=WetWare&diff=33576WetWare2019-08-19T15:51:25Z<p>Derishus: /* Molecular Biology */</p>
<hr />
<div>WetWare Shopping<br><br />
For independent scientists or artists doing DIYBio it is not always easy to get the biomaterials and wet ware easily. Here is a list of shops that are hacker-friendly and cheap:<br><br />
If you know a good store that you can recommend, please add it to the list.<br><br />
<br><br />
<br><br />
<blockquote>"If you start to order reagents yourself, start by ordering something innocuous. This will allow you to be a registered customer without raising red flags. Then graduate to ordering the harder stuff. "[https://medium.com/@Sachiko/lab-making-d573afa93231 Lab Making]</blockquote><br />
<br><br />
<br><br />
[[File:WetWare_s.jpg|600px]]<br />
==Chemistry==<br />
<br><br />
Chemistry, good quality, cheap and big choice from ebay:<br><br />
http://stores.ebay.de/s3chemicals/<br><br />
<br><br />
<br />
<br><br />
Poll on FaceBook by Alex Kelly:<br><br />
"Sunday morning question: What basic equipment/reagent/material have you struggled to obtain on the open consumer market?":<br><br />
<br><br />
* Sucrose and amino acids from sigma, "sent to US residential adress for a legit company - but they insisted on an official lab"<br><br />
* Chloral hydrate<br><br />
* Beakers<br><br />
* Methylamine<br><br />
* Love<br><br />
* Temperature regulators / thermostats. Julabo or Huber. "No second hand units available in Australia"<br><br />
* pure ethanol -"believe it or not".<br><br />
* Dichloromethane<br><br />
* The common inorganic acids (e.g. conc H2SO4)<br><br />
* Lithium acetate<br><br />
* Methacrylic anhydride<br><br />
* PEG in different molecular weights, PEG in 8000 and 3350<br><br />
* Glacial HOAc<br><br />
* Acetone AR<br><br />
* 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Is a photoinitiator in case you're wondering.)<br><br />
* NaOH solid (prills/pellets/whatever) (Must be AR, if I wanted Bunnings-grade I'd just buy it.)<br><br />
* A lot of the acetate salts (and acid) tend to pop up a lot in molecular biology and biochemistry in buffers etc.<br><br />
* HCl<br><br />
* H2SO4<br><br />
* Etehanol<br><br />
* CaCl2<br><br />
* MgCl2<br><br />
* Cost-effective DNA dyes such as ethidium / GelRed / SYBR / whatever<br><br />
* EDTA disodium dihydrate (sometimes people specify EDTA without specifying which salt and which hydrate, which is a pain because you don't specifically know what the counter ion is, or the molecular weight.)<br><br />
* Glycerol, DMSO, MgCl2, CaCl2, EDTA, Tris<br><br />
* Glycerol and DMSO<br><br />
* Pure NaCl<br><br />
* Agarose<br><br />
* Agar<br><br />
* Common media such as LB as well as individual components such as yeast extract and peptone etc.<br><br />
* Premix common buffers such as TAE<br><br />
* Common antibiotics e.g. ampicillin<br><br />
* Dextrose<br><br />
* L-Arabinose<br><br />
* Organic solvents that don't leave residue when evaporated<br><br />
* Ion-exchange resins for DNA workup, like Chelex sort of stuff.<br><br />
* Chloroform and phenol.<br><br />
* Isoamyl alcohol<br><br />
* PCR Mastermix<br><br />
* petridish<br><br />
* Schott bottles (or clones) in a spectrum of sizes including the little 100ml ones.<br><br />
* Stock microorganisms. Nothing dangerous but the common risk group 1 stuff. Like stock of Saccharomyces and K12.<br><br />
* strains of mice<br><br />
* Stock plasmids (e.g. common expression vectors, scaffolds, Cas9 templates)<br><br />
* monopotassium phosphate and dipotassium phosphate<br><br />
<br />
<br><br />
<br><br />
Thanks to everyone who helped compile this list...<br><br />
"Perhaps some of us should start a business selling scientific supplies, reagents etc marketed towards citizen scientist, hackers etc.. Could sell cheap kits for open source hardware too".. (Leo Mason)<br><br />
<br><br />
<br />
[http://www.sciencebuddies.org/science-fair-projects/project_ideas/Chem_Purchasing.shtml Chemical Purchasing] list on sciencebuddies.org.<br />
<br />
==Molecular Biology==<br />
The ODIN - The Open Discovery Institute - ebe:<br><br />
http://www.the-odin.com/<br><br />
<br><br />
Good quality TAQ and home brewing of biomaterials:<br><br />
https://www.geneandcell.com/<br><br />
<br><br />
Agarose and Tubes from ebay:<br><br />
http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48<br><br />
<br><br />
Petri Dishes, pipette tips, PCR Tubes and other stuff. (No small quantities. Price is OK, but not really cheap)<br><br />
Sarstedt, Switzerland:<br><br />
https://www.sarstedt.com/home/<br><br />
<br><br />
<br><br />
Laboratory Equipment, Life science, chemicals. (Not really cheap)<br><br />
Carl Roth, Switzerland<br><br />
https://www.carlroth.com/ch/de/<br><br />
<br><br />
<br><br />
Biohacker.jp articles (in Japanese) on [[http://biohacker.jp/c/BH230.html?fbclid=IwAR1xgRbhUHZUQg1BvWf76L9Cvz460dlBM5PWpjFWcDxtMYJC4fwfvxwYGts where to buy reagents]].<br />
<br />
==Plant Biology==<br />
[http://www.plantmedia.com/ Plant Media.com]<br />
[[Category:Wetware]]<br />
[[Category:Chemistry]]<br />
[[Category:Materials]]<br />
[[Category:Molecular]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=WetWare&diff=29935WetWare2019-02-04T16:46:25Z<p>Derishus: </p>
<hr />
<div>WetWare Shopping<br><br />
For independent scientists or artists doing DIYBio it is not always easy to get the biomaterials and wet ware easily. Here is a list of shops that are hacker-friendly and cheap:<br><br />
If you know a good store that you can recommend, please add it to the list.<br><br />
<br><br />
<br><br />
<blockquote>"If you start to order reagents yourself, start by ordering something innocuous. This will allow you to be a registered customer without raising red flags. Then graduate to ordering the harder stuff. "[https://medium.com/@Sachiko/lab-making-d573afa93231 Lab Making]</blockquote><br />
<br><br />
<br><br />
[[File:WetWare_s.jpg|600px]]<br />
==Chemistry==<br />
<br><br />
Chemistry, good quality, cheap and big choice from ebay:<br><br />
http://stores.ebay.de/s3chemicals/<br><br />
<br><br />
<br />
<br><br />
Poll on FaceBook by Alex Kelly:<br><br />
"Sunday morning question: What basic equipment/reagent/material have you struggled to obtain on the open consumer market?":<br><br />
<br><br />
* Sucrose and amino acids from sigma, "sent to US residential adress for a legit company - but they insisted on an official lab"<br><br />
* Chloral hydrate<br><br />
* Beakers<br><br />
* Methylamine<br><br />
* Love<br><br />
* Temperature regulators / thermostats. Julabo or Huber. "No second hand units available in Australia"<br><br />
* pure ethanol -"believe it or not".<br><br />
* Dichloromethane<br><br />
* The common inorganic acids (e.g. conc H2SO4)<br><br />
* Lithium acetate<br><br />
* Methacrylic anhydride<br><br />
* PEG in different molecular weights, PEG in 8000 and 3350<br><br />
* Glacial HOAc<br><br />
* Acetone AR<br><br />
* 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Is a photoinitiator in case you're wondering.)<br><br />
* NaOH solid (prills/pellets/whatever) (Must be AR, if I wanted Bunnings-grade I'd just buy it.)<br><br />
* A lot of the acetate salts (and acid) tend to pop up a lot in molecular biology and biochemistry in buffers etc.<br><br />
* HCl<br><br />
* H2SO4<br><br />
* Etehanol<br><br />
* CaCl2<br><br />
* MgCl2<br><br />
* Cost-effective DNA dyes such as ethidium / GelRed / SYBR / whatever<br><br />
* EDTA disodium dihydrate (sometimes people specify EDTA without specifying which salt and which hydrate, which is a pain because you don't specifically know what the counter ion is, or the molecular weight.)<br><br />
* Glycerol, DMSO, MgCl2, CaCl2, EDTA, Tris<br><br />
* Glycerol and DMSO<br><br />
* Pure NaCl<br><br />
* Agarose<br><br />
* Agar<br><br />
* Common media such as LB as well as individual components such as yeast extract and peptone etc.<br><br />
* Premix common buffers such as TAE<br><br />
* Common antibiotics e.g. ampicillin<br><br />
* Dextrose<br><br />
* L-Arabinose<br><br />
* Organic solvents that don't leave residue when evaporated<br><br />
* Ion-exchange resins for DNA workup, like Chelex sort of stuff.<br><br />
* Chloroform and phenol.<br><br />
* Isoamyl alcohol<br><br />
* PCR Mastermix<br><br />
* petridish<br><br />
* Schott bottles (or clones) in a spectrum of sizes including the little 100ml ones.<br><br />
* Stock microorganisms. Nothing dangerous but the common risk group 1 stuff. Like stock of Saccharomyces and K12.<br><br />
* strains of mice<br><br />
* Stock plasmids (e.g. common expression vectors, scaffolds, Cas9 templates)<br><br />
* monopotassium phosphate and dipotassium phosphate<br><br />
<br />
<br><br />
<br><br />
Thanks to everyone who helped compile this list...<br><br />
"Perhaps some of us should start a business selling scientific supplies, reagents etc marketed towards citizen scientist, hackers etc.. Could sell cheap kits for open source hardware too".. (Leo Mason)<br><br />
<br><br />
<br />
[http://www.sciencebuddies.org/science-fair-projects/project_ideas/Chem_Purchasing.shtml Chemical Purchasing] list on sciencebuddies.org.<br />
<br />
==Molecular Biology==<br />
The ODIN - The Open Discovery Institute - ebe:<br><br />
http://www.the-odin.com/<br><br />
<br><br />
Good quality TAQ and home brewing of biomaterials:<br><br />
https://www.geneandcell.com/<br><br />
<br><br />
Agarose and Tubes from ebay:<br><br />
http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48<br><br />
<br><br />
Petri Dishes, pipette tips, PCR Tubes and other stuff. (No small quantities. Price is OK, but not really cheap)<br><br />
Sarstedt, Switzerland:<br><br />
https://www.sarstedt.com/home/<br><br />
<br><br />
<br><br />
Laboratory Equipment, Life science, chemicals. (Not really cheap)<br><br />
Carl Roth, Switzerland<br><br />
https://www.carlroth.com/ch/de/<br><br />
<br><br />
<br><br />
<br />
==Plant Biology==<br />
[http://www.plantmedia.com/ Plant Media.com]<br />
[[Category:Wetware]]<br />
[[Category:Chemistry]]<br />
[[Category:Materials]]<br />
[[Category:Molecular]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=WetWare&diff=29934WetWare2019-02-04T16:45:43Z<p>Derishus: </p>
<hr />
<div>WetWare Shopping<br><br />
For independent scientists or artists doing DIYBio it is not always easy to get the biomaterials and wet ware easily. Here is a list of shops that are hacker-friendly and cheap:<br><br />
If you know a good store that you can recommend, please add it to the list.<br><br />
<br><br />
<br><br />
<blockquote>If you start to order reagents yourself, start by ordering something innocuous. This will allow you to be a registered customer without raising red flags. Then graduate to ordering the harder stuff. "[https://medium.com/@Sachiko/lab-making-d573afa93231 Lab Making]</blockquote><br />
<br><br />
<br><br />
[[File:WetWare_s.jpg|600px]]<br />
==Chemistry==<br />
<br><br />
Chemistry, good quality, cheap and big choice from ebay:<br><br />
http://stores.ebay.de/s3chemicals/<br><br />
<br><br />
<br />
<br><br />
Poll on FaceBook by Alex Kelly:<br><br />
"Sunday morning question: What basic equipment/reagent/material have you struggled to obtain on the open consumer market?":<br><br />
<br><br />
* Sucrose and amino acids from sigma, "sent to US residential adress for a legit company - but they insisted on an official lab"<br><br />
* Chloral hydrate<br><br />
* Beakers<br><br />
* Methylamine<br><br />
* Love<br><br />
* Temperature regulators / thermostats. Julabo or Huber. "No second hand units available in Australia"<br><br />
* pure ethanol -"believe it or not".<br><br />
* Dichloromethane<br><br />
* The common inorganic acids (e.g. conc H2SO4)<br><br />
* Lithium acetate<br><br />
* Methacrylic anhydride<br><br />
* PEG in different molecular weights, PEG in 8000 and 3350<br><br />
* Glacial HOAc<br><br />
* Acetone AR<br><br />
* 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Is a photoinitiator in case you're wondering.)<br><br />
* NaOH solid (prills/pellets/whatever) (Must be AR, if I wanted Bunnings-grade I'd just buy it.)<br><br />
* A lot of the acetate salts (and acid) tend to pop up a lot in molecular biology and biochemistry in buffers etc.<br><br />
* HCl<br><br />
* H2SO4<br><br />
* Etehanol<br><br />
* CaCl2<br><br />
* MgCl2<br><br />
* Cost-effective DNA dyes such as ethidium / GelRed / SYBR / whatever<br><br />
* EDTA disodium dihydrate (sometimes people specify EDTA without specifying which salt and which hydrate, which is a pain because you don't specifically know what the counter ion is, or the molecular weight.)<br><br />
* Glycerol, DMSO, MgCl2, CaCl2, EDTA, Tris<br><br />
* Glycerol and DMSO<br><br />
* Pure NaCl<br><br />
* Agarose<br><br />
* Agar<br><br />
* Common media such as LB as well as individual components such as yeast extract and peptone etc.<br><br />
* Premix common buffers such as TAE<br><br />
* Common antibiotics e.g. ampicillin<br><br />
* Dextrose<br><br />
* L-Arabinose<br><br />
* Organic solvents that don't leave residue when evaporated<br><br />
* Ion-exchange resins for DNA workup, like Chelex sort of stuff.<br><br />
* Chloroform and phenol.<br><br />
* Isoamyl alcohol<br><br />
* PCR Mastermix<br><br />
* petridish<br><br />
* Schott bottles (or clones) in a spectrum of sizes including the little 100ml ones.<br><br />
* Stock microorganisms. Nothing dangerous but the common risk group 1 stuff. Like stock of Saccharomyces and K12.<br><br />
* strains of mice<br><br />
* Stock plasmids (e.g. common expression vectors, scaffolds, Cas9 templates)<br><br />
* monopotassium phosphate and dipotassium phosphate<br><br />
<br />
<br><br />
<br><br />
Thanks to everyone who helped compile this list...<br><br />
"Perhaps some of us should start a business selling scientific supplies, reagents etc marketed towards citizen scientist, hackers etc.. Could sell cheap kits for open source hardware too".. (Leo Mason)<br><br />
<br><br />
<br />
[http://www.sciencebuddies.org/science-fair-projects/project_ideas/Chem_Purchasing.shtml Chemical Purchasing] list on sciencebuddies.org.<br />
<br />
==Molecular Biology==<br />
The ODIN - The Open Discovery Institute - ebe:<br><br />
http://www.the-odin.com/<br><br />
<br><br />
Good quality TAQ and home brewing of biomaterials:<br><br />
https://www.geneandcell.com/<br><br />
<br><br />
Agarose and Tubes from ebay:<br><br />
http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48<br><br />
<br><br />
Petri Dishes, pipette tips, PCR Tubes and other stuff. (No small quantities. Price is OK, but not really cheap)<br><br />
Sarstedt, Switzerland:<br><br />
https://www.sarstedt.com/home/<br><br />
<br><br />
<br><br />
Laboratory Equipment, Life science, chemicals. (Not really cheap)<br><br />
Carl Roth, Switzerland<br><br />
https://www.carlroth.com/ch/de/<br><br />
<br><br />
<br><br />
<br />
==Plant Biology==<br />
[http://www.plantmedia.com/ Plant Media.com]<br />
[[Category:Wetware]]<br />
[[Category:Chemistry]]<br />
[[Category:Materials]]<br />
[[Category:Molecular]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=WetWare&diff=29933WetWare2019-02-04T16:45:11Z<p>Derishus: </p>
<hr />
<div>WetWare Shopping<br><br />
For independent scientists or artists doing DIYBio it is not always easy to get the biomaterials and wet ware easily. Here is a list of shops that are hacker-friendly and cheap:<br><br />
If you know a good store that you can recommend, please add it to the list.<br><br />
<br><br />
<br><br />
<blockquote>"If you start to order reagents yourself, start by ordering something innocuous. This will allow you to be a registered customer without raising red flags. Then graduate to ordering the harder stuff. "[https://medium.com/@Sachiko/lab-making-d573afa93231 Lab Making]</blockquote><br />
[[File:WetWare_s.jpg|600px]]<br />
==Chemistry==<br />
<br><br />
Chemistry, good quality, cheap and big choice from ebay:<br><br />
http://stores.ebay.de/s3chemicals/<br><br />
<br><br />
<br />
<br><br />
Poll on FaceBook by Alex Kelly:<br><br />
"Sunday morning question: What basic equipment/reagent/material have you struggled to obtain on the open consumer market?":<br><br />
<br><br />
* Sucrose and amino acids from sigma, "sent to US residential adress for a legit company - but they insisted on an official lab"<br><br />
* Chloral hydrate<br><br />
* Beakers<br><br />
* Methylamine<br><br />
* Love<br><br />
* Temperature regulators / thermostats. Julabo or Huber. "No second hand units available in Australia"<br><br />
* pure ethanol -"believe it or not".<br><br />
* Dichloromethane<br><br />
* The common inorganic acids (e.g. conc H2SO4)<br><br />
* Lithium acetate<br><br />
* Methacrylic anhydride<br><br />
* PEG in different molecular weights, PEG in 8000 and 3350<br><br />
* Glacial HOAc<br><br />
* Acetone AR<br><br />
* 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Is a photoinitiator in case you're wondering.)<br><br />
* NaOH solid (prills/pellets/whatever) (Must be AR, if I wanted Bunnings-grade I'd just buy it.)<br><br />
* A lot of the acetate salts (and acid) tend to pop up a lot in molecular biology and biochemistry in buffers etc.<br><br />
* HCl<br><br />
* H2SO4<br><br />
* Etehanol<br><br />
* CaCl2<br><br />
* MgCl2<br><br />
* Cost-effective DNA dyes such as ethidium / GelRed / SYBR / whatever<br><br />
* EDTA disodium dihydrate (sometimes people specify EDTA without specifying which salt and which hydrate, which is a pain because you don't specifically know what the counter ion is, or the molecular weight.)<br><br />
* Glycerol, DMSO, MgCl2, CaCl2, EDTA, Tris<br><br />
* Glycerol and DMSO<br><br />
* Pure NaCl<br><br />
* Agarose<br><br />
* Agar<br><br />
* Common media such as LB as well as individual components such as yeast extract and peptone etc.<br><br />
* Premix common buffers such as TAE<br><br />
* Common antibiotics e.g. ampicillin<br><br />
* Dextrose<br><br />
* L-Arabinose<br><br />
* Organic solvents that don't leave residue when evaporated<br><br />
* Ion-exchange resins for DNA workup, like Chelex sort of stuff.<br><br />
* Chloroform and phenol.<br><br />
* Isoamyl alcohol<br><br />
* PCR Mastermix<br><br />
* petridish<br><br />
* Schott bottles (or clones) in a spectrum of sizes including the little 100ml ones.<br><br />
* Stock microorganisms. Nothing dangerous but the common risk group 1 stuff. Like stock of Saccharomyces and K12.<br><br />
* strains of mice<br><br />
* Stock plasmids (e.g. common expression vectors, scaffolds, Cas9 templates)<br><br />
* monopotassium phosphate and dipotassium phosphate<br><br />
<br />
<br><br />
<br><br />
Thanks to everyone who helped compile this list...<br><br />
"Perhaps some of us should start a business selling scientific supplies, reagents etc marketed towards citizen scientist, hackers etc.. Could sell cheap kits for open source hardware too".. (Leo Mason)<br><br />
<br><br />
<br />
[http://www.sciencebuddies.org/science-fair-projects/project_ideas/Chem_Purchasing.shtml Chemical Purchasing] list on sciencebuddies.org.<br />
<br />
==Molecular Biology==<br />
The ODIN - The Open Discovery Institute - ebe:<br><br />
http://www.the-odin.com/<br><br />
<br><br />
Good quality TAQ and home brewing of biomaterials:<br><br />
https://www.geneandcell.com/<br><br />
<br><br />
Agarose and Tubes from ebay:<br><br />
http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48<br><br />
<br><br />
Petri Dishes, pipette tips, PCR Tubes and other stuff. (No small quantities. Price is OK, but not really cheap)<br><br />
Sarstedt, Switzerland:<br><br />
https://www.sarstedt.com/home/<br><br />
<br><br />
<br><br />
Laboratory Equipment, Life science, chemicals. (Not really cheap)<br><br />
Carl Roth, Switzerland<br><br />
https://www.carlroth.com/ch/de/<br><br />
<br><br />
<br><br />
<br />
==Plant Biology==<br />
[http://www.plantmedia.com/ Plant Media.com]<br />
[[Category:Wetware]]<br />
[[Category:Chemistry]]<br />
[[Category:Materials]]<br />
[[Category:Molecular]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=29733Worm Composting2018-12-06T12:04:59Z<p>Derishus: /* Links */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
* '''Esophagus''' : Calcium Carbonate secretion to remove excess calcium from the body, and neutralize acidic food<br />
* '''Gizzard''' : This is where the food is mashed physically by sand<br />
* '''Intestines''' : This is where enzymatic and microbial processing of the ingested foodstuff gets digested<br />
** mucus containing protein and polysaccharides<br />
*** digestive enzymes such as amylase, cellulase, protease, lipase, chitinase and urease<br />
** organic and mineral matter<br />
** amino acids <br />
** '''It's not only worms''' - microbial symbionts (bacteria, protozoa and microfungi) - also responsible for some of the digestive enzymes)<br />
* '''Anus''' : This is where the processed worm castings (waste, i.e. poop) comes out. One man's waste is another one's gold!<br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
<br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
<br />
For ''E. foetida'' reproduction, taken from [http://www.sierra-worm-compost.com/composting-worms.html Sierra worm compost]<br><br />
* Produce 3.8 cocoon per adult per week.<br />
* Each cocoon contains between 8-20 eggs, but on average only 3.3 worms will actually hatch.<br />
* Hatching occurs in about 3 weeks.<br />
* 53-76 days to sexual maturity.<br />
* 85-149 days from egg to maturity.<br />
<br />
<br><br><br />
This [https://youtu.be/KbhjqMngGz4 video] shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Science<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
* [https://animals.howstuffworks.com/animal-facts/earthworm.htm How Stuff Works, Earth Worm]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
=== Nicely Designed Worm Compost Bins ===<br />
* [https://eng.wormup.ch/ wormup] makes them in sustainable ceramic!<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Circular_Chromatography&diff=29434Circular Chromatography2018-09-30T09:49:09Z<p>Derishus: </p>
<hr />
<div><br />
==Introduction==<br />
[http://www.dictionary.com/browse/chromato- CHROMATO] = colour; of or in colours<br><br />
[http://www.dictionary.com/browse/graphy?s=t GRAPHY] = writing...representing...or a process, or art / science working with such a process<br />
<br />
Chromatography is a way to analyse what is in a mixture (in this case liquid) - by separation on solid support (in this case the fibers in the paper), and visualization in this case, by a [https://en.wikipedia.org/wiki/Photographic_processing photographic process].<br><br />
<br />
"The mixture flows though a medium and the individual substances that make up the mixture are deposited at different distances from the point of inflow." - from website of [http://biodynamics.in/chrom.htm Bio-dynamic Society of India]<br />
The separation happens as the liquid slowly wets the paper by [https://en.wikipedia.org/wiki/Capillary_action capillary action] - <br><br />
Those compounds that stick to the paper (medium/ '''stationary / solid phase'''), will stay closer to the point of inflow.<br><br />
Those compounds that prefer the liquid ('''solvent''', '''mobile phase'''), it will travel further with the liquid.<br><br />
This process can just be called [https://en.wikipedia.org/wiki/Paper_chromatography paper chromatography]. Because we use paper as a support.Because we did this on a circular piece of paper, it is called circular chromatography. <br><br />
<br />
<br><br />
In this case, we took some soil and mud samples we had collected in different water bodies locations, and tried to analyse the humic content of the samples.<br><br />
This method can be applied to any other liquid mixtures.<br><br />
For example, [https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=0ahUKEwizscXL-qDTAhXEpo8KHdVgC-8QFggkMAA&url=http%3A%2F%2Fjournal.iisc.ernet.in%2Findex.php%2Fiisc%2Farticle%2Fdownload%2F2786%2F4173&usg=AFQjCNE4N72bxFl2hwdMwSm47mFqaQ9xZw&sig2=ikNpi2l8nfCn-YukCpOseA&cad=rja this 1953 paper] uses butanol-based solvents to separate different sugars, and visualizes the different sugars. <br><br />
[https://en.wikipedia.org/wiki/Benedict%27s_reagent Benedict's reagent] may be a nice DIY mix for looking for reducing sugars.<br><br><br />
<br />
To learn more, here is the Khan Academy's explanation of the [https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/a/principles-of-chromatography Principles of Chromatography].<br />
<br><br><br />
<br />
==Methods==<br />
===[http://biodynamics.in/chrom.htm BIO-DYNAMIC ASSOCIATION OF INDIA]===<br />
This is based on our experience trying out the recommended protocol from the page of [http://biodynamics.in/chrom.htm BIO-DYNAMIC ASSOCIATION OF INDIA]<br><br />
The original steps from [http://biodynamics.in/chrom.htm Biodynamic Association of India]:<br />
<br />
1. A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter. <br />
2. The wick is removed and the paper is dried.<br />
3. Meanwhile, the substance to be tested is mixed with a 0.1 to 1% solution of sodium hydroxide and let stand for a period of time. <br />
4. The prepared filter paper is then allowed to absorb this solution and the substance spreads over the paper. <br />
5. When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop. <br />
<br><br />
<br />
====Materials====<br />
----<br />
'''Chemicals''' (!! again, this depends on what you want to separate, and what compounds you want to see)<br />
* '''for visualization''' - 0.5% solution of silver nitrate (you can find in photography suppliers)<br />
* '''solvent, mobile phase''' - 0.1 to 1% solution of [https://en.wikipedia.org/wiki/Sodium_hydroxide sodium hydroxide] (NaOH, otherwise known as lye or caustic soda - be careful, because it can burn)<br />
'''Other materials'''<br />
* paper (Whatman, or any acid-free thick paper)<br />
* reservoir<br />
* wick (medical cotton, and rolled it into a wick)<br />
* light for development - sunlight is fine unless controlled exposure is wanted<br />
<br><br />
<br />
====Step by Step====<br />
----<br />
<br />
'''DAY1'''<br><br />
=====Extraction=====<br />
[[File:CC_sample_extraction.png|400px|right]]<br />
<p><br />
This is the step where we take our samples we want to analyse, and extract what is in it into a liquid phase.<br />
</p><br />
<br />
<p><br />
Just like taking a crushed piece of garlic, and putting it into water or oil, brings out different flavors in the water and oil, we decided to see what will be extracted from the soil samples when using 2 different solutions.<br />
</p><br />
::* 0.2% NaOH in water<br />
::* isopropyl alcohol<br />
To learn more, here is a [https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/v/extractions Khan Academy video on extractions].<br><br />
!!!!Dilute NaOH is used to maximize the [http://karnet.up.wroc.pl/~weber/ekstrak2.htm recovery of humic substances from soil]. For analysis of other compounds, make sure to use appropriate extraction liquid.<br />
<br><br><br />
<p><br />
# Take one spoon scoop of soil/mud into petridish to mix and extract with NaOH.<br><br />
# The same scoop of soil/mud into a tube to extract with isoproypl alcohol (it evaporates faster). Here, it may be best to put both in a closeable container, so we can mix and shake the samples.<br><br />
# Leave the samples overnight at room temperature.<br><br />
</p><br />
<br><br />
<p><br />
:: For better controlled experiments -<br />
::* Weigh out equal amounts of soil<br />
::* Shake constantly<br />
::* Constant temperature during extraction<br />
::* Consistent amount of time during extraction<br />
</p><br />
<br><br />
'''DAY2'''<br><br />
The next steps needs to happen in '''a darkroom''' with a red lamp.<br><br><br />
<gallery><br />
File:CC_silvernitrate.png|Preparation of light sensitive paper<br />
File:800px-CC_sample_application.JPG|Sample application<br />
File:800px-CC_exposure_to_light.JPG|Exposure to light<br />
</gallery><br />
<br />
=====Preparation of Light sensitive paper=====<br />
# Make the 0.5% silver nitrate solution<br />
# Place 2mL of the 0.5% silver nitrate solution in a clean container<br />
# Place a short wick through the middle of the filter paper<br />
# Place the wick in the silver nitrate<br />
# Wait til the silver nitrate circle is about 1cm from the edge<br />
# Remove the wick<br />
# Let the filter paper dry.<br />
<br />
=====Sample application=====<br />
# Add a new wick on the prepared paper above<br />
# Take 2mL the liquid part of the samples prepared above, and place in a cleaned container<br />
# Let the sample travel til just before the silver nitrate circle<br />
# Remove wick <br />
# Let the filter dry<br />
[[File:Dipped paper.JPG|640px]]<br />
<br />
=====Exposure to see the different bands=====<br />
# Take the filters and expose to indirect light, or in more controlled situations, use a timer and a light set up<br />
# et voila! time for analysis<br />
<br />
<br><br />
====Analysis of the Chromatogram====<br />
The analysis of the final chromatogram - the filter paper - seems more difficult.<br><br />
Comparison between before and after, different locations, may be easier than to pin-point exactly what is producing the patterns.<br><br />
See one explanation at the bottom of this [http://www.biodynamics.in/chrom.htm webpage].<br />
<br><br />
The different colours we obtain has to be a reflection of the [http://www.saltlakemetals.com/Silver_Nitrate_Uses.htm chemical reaction with silver nitrate] and whatever compound that is deposited there. (It seems to be copied from [https://www.scrapmetalforum.com/scrap-metal-tips-advice/13866-silver-what-used-most-useful-all-noble-metals.html here])<br />
<br><br><br />
For Halides<br><br />
For example: <br><br />
* White=Chloride<br />
* Pale Yellow=Bromide<br />
* Yellow=Iodide.<br />
Test for the presence of Carbonate, Hydroxide, Sulfide and Phosphate ions.<br><br />
For example: <br><br />
* Pale Green=Carbonate<br />
* Brown=Hydroxide<br />
* Black=Sulfide.<br />
<br><br><br />
<br />
===Humus Sapiens 2018 Workshop by [https://www.facebook.com/emanuela.ascari Emanuela]===<br />
<br />
<br><br><br />
<br />
===Choosing Appropriate Methods===<br />
----<br />
<br />
*Bio Crystallisation, copper chloride crystallisation, proteins<br />
*Capillary Dynamolysis, vertical filters, ‘rising pictures’, sugars/ bitter materials<br />
*Bio Chromatography, horizontal circular images, minerals, sugars/ bitter materials<br />
<br />
#[http://www.biodynamic-research.net/ras/rm/pfm Description of methods]<br />
#[http://biodyn.se/en/bildskapande-forskningsmetoder/ Choosing appropriate methods]<br />
<br><br><br />
<br />
==Resources/Links==<br />
#[http://www.dynamis.tv/fr/cristallisation-sensible-morphochromatographie-biodynamie.php Chromatograpy of wine] <br />
#[http://www.levity.com/alchemy/kolisko.html Capillary Dynamolysis] <br />
#[http://biodyn.se/en/forskning-pagar/ Scandenavian Biodynamics Research] <br />
#[https://www.omicsgroup.org/journals/2165-7866/2165-7866-1-101.php?%252520aid=2429 Academic paper on Capillary Dynamolysis Image Discrimination Using Neural Networks] <br />
#[https://ledepotesta.wordpress.com/2016/01/20/koliskos-agriculture-of-tomorrow-pt-2/#more-133 Summary of Kolisko's book] <br />
#[http://www.sciencegroup.org.uk/history.htm History of Science Group of the Anthroposophical Society in Great Britain]<br />
#[https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/a/principles-of-chromatography Khan Academy Principles of Chromatography]<br />
#[http://karnet.up.wroc.pl/~weber/ekstrak2.htm Extraction of Soil Organic Matter]<br />
#[http://lab-training.com/2014/11/21/types-paper-chromatography/ Types of Paper Chromatography]<br />
[[Category:Analysis]][[Category:Environment]][[Category:Wetware]][[Category:Chemistry]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Circular_Chromatography&diff=29433Circular Chromatography2018-09-30T09:47:48Z<p>Derishus: </p>
<hr />
<div><br />
==Introduction==<br />
[http://www.dictionary.com/browse/chromato- CHROMATO] = colour; of or in colours<br><br />
[http://www.dictionary.com/browse/graphy?s=t GRAPHY] = writing...representing...or a process, or art / science working with such a process<br />
<br />
Chromatography is a way to analyse what is in a mixture (in this case liquid) - by separation on solid support (in this case the fibers in the paper), and visualization in this case, by a [https://en.wikipedia.org/wiki/Photographic_processing photographic process].<br><br />
<br />
"The mixture flows though a medium and the individual substances that make up the mixture are deposited at different distances from the point of inflow." - from website of [http://biodynamics.in/chrom.htm Bio-dynamic Society of India]<br />
The separation happens as the liquid slowly wets the paper by [https://en.wikipedia.org/wiki/Capillary_action capillary action] - <br><br />
Those compounds that stick to the paper (medium/ '''stationary / solid phase'''), will stay closer to the point of inflow.<br><br />
Those compounds that prefer the liquid ('''solvent''', '''mobile phase'''), it will travel further with the liquid.<br><br />
This process can just be called [https://en.wikipedia.org/wiki/Paper_chromatography paper chromatography]. Because we use paper as a support.Because we did this on a circular piece of paper, it is called circular chromatography. <br><br />
<br />
<br><br />
In this case, we took some soil and mud samples we had collected in different water bodies locations, and tried to analyse the humic content of the samples.<br><br />
This method can be applied to any other liquid mixtures.<br><br />
For example, [https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=0ahUKEwizscXL-qDTAhXEpo8KHdVgC-8QFggkMAA&url=http%3A%2F%2Fjournal.iisc.ernet.in%2Findex.php%2Fiisc%2Farticle%2Fdownload%2F2786%2F4173&usg=AFQjCNE4N72bxFl2hwdMwSm47mFqaQ9xZw&sig2=ikNpi2l8nfCn-YukCpOseA&cad=rja this 1953 paper] uses butanol-based solvents to separate different sugars, and visualizes the different sugars. <br><br />
[https://en.wikipedia.org/wiki/Benedict%27s_reagent Benedict's reagent] may be a nice DIY mix for looking for reducing sugars.<br><br><br />
<br />
To learn more, here is the Khan Academy's explanation of the [https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/a/principles-of-chromatography Principles of Chromatography].<br />
<br><br><br />
<br />
==Methods==<br />
===[http://biodynamics.in/chrom.htm BIO-DYNAMIC ASSOCIATION OF INDIA]===<br />
This is based on our experience trying out the recommended protocol from the page of [http://biodynamics.in/chrom.htm BIO-DYNAMIC ASSOCIATION OF INDIA]<br><br />
The original steps from [http://biodynamics.in/chrom.htm Biodynamic Association of India]:<br />
<br />
1. A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter. <br />
2. The wick is removed and the paper is dried.<br />
3. Meanwhile, the substance to be tested is mixed with a 0.1 to 1% solution of sodium hydroxide and let stand for a period of time. <br />
4. The prepared filter paper is then allowed to absorb this solution and the substance spreads over the paper. <br />
5. When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop. <br />
<br><br />
<br />
====Materials====<br />
----<br />
'''Chemicals''' (!! again, this depends on what you want to separate, and what compounds you want to see)<br />
* '''for visualization''' - 0.5% solution of silver nitrate (you can find in photography suppliers)<br />
* '''solvent, mobile phase''' - 0.1 to 1% solution of [https://en.wikipedia.org/wiki/Sodium_hydroxide sodium hydroxide] (NaOH, otherwise known as lye or caustic soda - be careful, because it can burn)<br />
'''Other materials'''<br />
* paper (Whatman, or any acid-free thick paper)<br />
* reservoir<br />
* wick (medical cotton, and rolled it into a wick)<br />
* light for development - sunlight is fine unless controlled exposure is wanted<br />
<br><br />
<br />
====Step by Step====<br />
----<br />
<br />
'''DAY1'''<br><br />
=====Extraction=====<br />
[[File:CC_sample_extraction.png|400px|right]]<br />
<p><br />
This is the step where we take our samples we want to analyse, and extract what is in it into a liquid phase.<br />
</p><br />
<br />
<p><br />
Just like taking a crushed piece of garlic, and putting it into water or oil, brings out different flavors in the water and oil, we decided to see what will be extracted from the soil samples when using 2 different solutions.<br />
</p><br />
::* 0.2% NaOH in water<br />
::* isopropyl alcohol<br />
To learn more, here is a [https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/v/extractions Khan Academy video on extractions].<br><br />
!!!!Dilute NaOH is used to maximize the [http://karnet.up.wroc.pl/~weber/ekstrak2.htm recovery of humic substances from soil]. For analysis of other compounds, make sure to use appropriate extraction liquid.<br />
<br><br><br />
<p><br />
# Take one spoon scoop of soil/mud into petridish to mix and extract with NaOH.<br><br />
# The same scoop of soil/mud into a tube to extract with isoproypl alcohol (it evaporates faster). Here, it may be best to put both in a closeable container, so we can mix and shake the samples.<br><br />
# Leave the samples overnight at room temperature.<br><br />
</p><br />
<br><br />
<p><br />
:: For better controlled experiments -<br />
::* Weigh out equal amounts of soil<br />
::* Shake constantly<br />
::* Constant temperature during extraction<br />
::* Consistent amount of time during extraction<br />
</p><br />
<br><br />
'''DAY2'''<br><br />
The next steps needs to happen in '''a darkroom''' with a red lamp.<br><br><br />
<gallery><br />
File:CC_silvernitrate.png|Preparation of light sensitive paper<br />
File:800px-CC_sample_application.JPG|Sample application<br />
File:800px-CC_exposure_to_light.JPG|Exposure to light<br />
</gallery><br />
<br />
=====Preparation of Light sensitive paper=====<br />
# Make the 0.5% silver nitrate solution<br />
# Place 2mL of the 0.5% silver nitrate solution in a clean container<br />
# Place a short wick through the middle of the filter paper<br />
# Place the wick in the silver nitrate<br />
# Wait til the silver nitrate circle is about 1cm from the edge<br />
# Remove the wick<br />
# Let the filter paper dry.<br />
<br />
=====Sample application=====<br />
# Add a new wick on the prepared paper above<br />
# Take 2mL the liquid part of the samples prepared above, and place in a cleaned container<br />
# Let the sample travel til just before the silver nitrate circle<br />
# Remove wick <br />
# Let the filter dry<br />
[[File:Dipped paper.JPG|640px]]<br />
<br />
=====Exposure to see the different bands=====<br />
# Take the filters and expose to indirect light, or in more controlled situations, use a timer and a light set up<br />
# et voila! time for analysis<br />
<br />
<br><br />
====Analysis of the Chromatogram====<br />
The analysis of the final chromatogram - the filter paper - seems more difficult.<br><br />
Comparison between before and after, different locations, may be easier than to pin-point exactly what is producing the patterns.<br><br />
See one explanation at the bottom of this [http://www.biodynamics.in/chrom.htm webpage].<br />
<br><br />
The different colours we obtain has to be a reflection of the [http://www.saltlakemetals.com/Silver_Nitrate_Uses.htm chemical reaction with silver nitrate] and whatever compound that is deposited there. (It seems to be copied from [https://www.scrapmetalforum.com/scrap-metal-tips-advice/13866-silver-what-used-most-useful-all-noble-metals.html here])<br />
<br><br><br />
For Halides<br><br />
For example: <br><br />
* White=Chloride<br />
* Pale Yellow=Bromide<br />
* Yellow=Iodide.<br />
Test for the presence of Carbonate, Hydroxide, Sulfide and Phosphate ions.<br><br />
For example: <br><br />
* Pale Green=Carbonate<br />
* Brown=Hydroxide<br />
* Black=Sulfide.<br />
<br><br><br />
<br />
===Humus Sapiens 2018 Workshop by [https://www.facebook.com/emanuela.ascari Emanuela]===<br />
<br />
<br />
<br />
<br />
----<br />
<br />
*Bio Crystallisation, copper chloride crystallisation, proteins<br />
*Capillary Dynamolysis, vertical filters, ‘rising pictures’, sugars/ bitter materials<br />
*Bio Chromatography, horizontal circular images, minerals, sugars/ bitter materials<br />
<br />
#[http://www.biodynamic-research.net/ras/rm/pfm Description of methods]<br />
#[http://biodyn.se/en/bildskapande-forskningsmetoder/ Choosing appropriate methods]<br />
<br><br><br />
<br />
==Resources/Links==<br />
#[http://www.dynamis.tv/fr/cristallisation-sensible-morphochromatographie-biodynamie.php Chromatograpy of wine] <br />
#[http://www.levity.com/alchemy/kolisko.html Capillary Dynamolysis] <br />
#[http://biodyn.se/en/forskning-pagar/ Scandenavian Biodynamics Research] <br />
#[https://www.omicsgroup.org/journals/2165-7866/2165-7866-1-101.php?%252520aid=2429 Academic paper on Capillary Dynamolysis Image Discrimination Using Neural Networks] <br />
#[https://ledepotesta.wordpress.com/2016/01/20/koliskos-agriculture-of-tomorrow-pt-2/#more-133 Summary of Kolisko's book] <br />
#[http://www.sciencegroup.org.uk/history.htm History of Science Group of the Anthroposophical Society in Great Britain]<br />
#[https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/a/principles-of-chromatography Khan Academy Principles of Chromatography]<br />
#[http://karnet.up.wroc.pl/~weber/ekstrak2.htm Extraction of Soil Organic Matter]<br />
#[http://lab-training.com/2014/11/21/types-paper-chromatography/ Types of Paper Chromatography]<br />
[[Category:Analysis]][[Category:Environment]][[Category:Wetware]][[Category:Chemistry]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Circular_Chromatography&diff=29432Circular Chromatography2018-09-30T09:47:09Z<p>Derishus: /* Methods */</p>
<hr />
<div><br />
==Introduction==<br />
[http://www.dictionary.com/browse/chromato- CHROMATO] = colour; of or in colours<br><br />
[http://www.dictionary.com/browse/graphy?s=t GRAPHY] = writing...representing...or a process, or art / science working with such a process<br />
<br />
Chromatography is a way to analyse what is in a mixture (in this case liquid) - by separation on solid support (in this case the fibers in the paper), and visualization in this case, by a [https://en.wikipedia.org/wiki/Photographic_processing photographic process].<br><br />
<br />
"The mixture flows though a medium and the individual substances that make up the mixture are deposited at different distances from the point of inflow." - from website of [http://biodynamics.in/chrom.htm Bio-dynamic Society of India]<br />
The separation happens as the liquid slowly wets the paper by [https://en.wikipedia.org/wiki/Capillary_action capillary action] - <br><br />
Those compounds that stick to the paper (medium/ '''stationary / solid phase'''), will stay closer to the point of inflow.<br><br />
Those compounds that prefer the liquid ('''solvent''', '''mobile phase'''), it will travel further with the liquid.<br><br />
This process can just be called [https://en.wikipedia.org/wiki/Paper_chromatography paper chromatography]. Because we use paper as a support.Because we did this on a circular piece of paper, it is called circular chromatography. <br><br />
<br />
<br><br />
In this case, we took some soil and mud samples we had collected in different water bodies locations, and tried to analyse the humic content of the samples.<br><br />
This method can be applied to any other liquid mixtures.<br><br />
For example, [https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=0ahUKEwizscXL-qDTAhXEpo8KHdVgC-8QFggkMAA&url=http%3A%2F%2Fjournal.iisc.ernet.in%2Findex.php%2Fiisc%2Farticle%2Fdownload%2F2786%2F4173&usg=AFQjCNE4N72bxFl2hwdMwSm47mFqaQ9xZw&sig2=ikNpi2l8nfCn-YukCpOseA&cad=rja this 1953 paper] uses butanol-based solvents to separate different sugars, and visualizes the different sugars. <br><br />
[https://en.wikipedia.org/wiki/Benedict%27s_reagent Benedict's reagent] may be a nice DIY mix for looking for reducing sugars.<br><br><br />
<br />
To learn more, here is the Khan Academy's explanation of the [https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/a/principles-of-chromatography Principles of Chromatography].<br />
<br><br><br />
<br />
==Methods==<br />
===[http://biodynamics.in/chrom.htm BIO-DYNAMIC ASSOCIATION OF INDIA]===<br />
This is based on our experience trying out the recommended protocol from the page of [http://biodynamics.in/chrom.htm BIO-DYNAMIC ASSOCIATION OF INDIA]<br><br />
The original steps from [http://biodynamics.in/chrom.htm Biodynamic Association of India]:<br />
<br />
1. A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter. <br />
2. The wick is removed and the paper is dried.<br />
3. Meanwhile, the substance to be tested is mixed with a 0.1 to 1% solution of sodium hydroxide and let stand for a period of time. <br />
4. The prepared filter paper is then allowed to absorb this solution and the substance spreads over the paper. <br />
5. When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop. <br />
<br><br />
<br />
====Materials====<br />
----<br />
'''Chemicals''' (!! again, this depends on what you want to separate, and what compounds you want to see)<br />
* '''for visualization''' - 0.5% solution of silver nitrate (you can find in photography suppliers)<br />
* '''solvent, mobile phase''' - 0.1 to 1% solution of [https://en.wikipedia.org/wiki/Sodium_hydroxide sodium hydroxide] (NaOH, otherwise known as lye or caustic soda - be careful, because it can burn)<br />
'''Other materials'''<br />
* paper (Whatman, or any acid-free thick paper)<br />
* reservoir<br />
* wick (medical cotton, and rolled it into a wick)<br />
* light for development - sunlight is fine unless controlled exposure is wanted<br />
<br><br />
<br />
====Step by Step====<br />
----<br />
<br />
'''DAY1'''<br><br />
=====Extraction=====<br />
[[File:CC_sample_extraction.png|400px|right]]<br />
<p><br />
This is the step where we take our samples we want to analyse, and extract what is in it into a liquid phase.<br />
</p><br />
<br />
<p><br />
Just like taking a crushed piece of garlic, and putting it into water or oil, brings out different flavors in the water and oil, we decided to see what will be extracted from the soil samples when using 2 different solutions.<br />
</p><br />
::* 0.2% NaOH in water<br />
::* isopropyl alcohol<br />
To learn more, here is a [https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/v/extractions Khan Academy video on extractions].<br><br />
!!!!Dilute NaOH is used to maximize the [http://karnet.up.wroc.pl/~weber/ekstrak2.htm recovery of humic substances from soil]. For analysis of other compounds, make sure to use appropriate extraction liquid.<br />
<br><br><br />
<p><br />
# Take one spoon scoop of soil/mud into petridish to mix and extract with NaOH.<br><br />
# The same scoop of soil/mud into a tube to extract with isoproypl alcohol (it evaporates faster). Here, it may be best to put both in a closeable container, so we can mix and shake the samples.<br><br />
# Leave the samples overnight at room temperature.<br><br />
</p><br />
<br><br />
<p><br />
:: For better controlled experiments -<br />
::* Weigh out equal amounts of soil<br />
::* Shake constantly<br />
::* Constant temperature during extraction<br />
::* Consistent amount of time during extraction<br />
</p><br />
<br><br />
'''DAY2'''<br><br />
The next steps needs to happen in '''a darkroom''' with a red lamp.<br><br><br />
<gallery><br />
File:CC_silvernitrate.png|Preparation of light sensitive paper<br />
File:800px-CC_sample_application.JPG|Sample application<br />
File:800px-CC_exposure_to_light.JPG|Exposure to light<br />
</gallery><br />
<br />
=====Preparation of Light sensitive paper=====<br />
# Make the 0.5% silver nitrate solution<br />
# Place 2mL of the 0.5% silver nitrate solution in a clean container<br />
# Place a short wick through the middle of the filter paper<br />
# Place the wick in the silver nitrate<br />
# Wait til the silver nitrate circle is about 1cm from the edge<br />
# Remove the wick<br />
# Let the filter paper dry.<br />
<br />
=====Sample application=====<br />
# Add a new wick on the prepared paper above<br />
# Take 2mL the liquid part of the samples prepared above, and place in a cleaned container<br />
# Let the sample travel til just before the silver nitrate circle<br />
# Remove wick <br />
# Let the filter dry<br />
[[File:Dipped paper.JPG|640px]]<br />
<br />
=====Exposure to see the different bands=====<br />
# Take the filters and expose to indirect light, or in more controlled situations, use a timer and a light set up<br />
# et voila! time for analysis<br />
<br />
<br><br />
<br />
===Humus Sapiens 2018 Workshop by [https://www.facebook.com/emanuela.ascari Emanuela]===<br />
<br />
====Analysis of the Chromatogram====<br />
The analysis of the final chromatogram - the filter paper - seems more difficult.<br><br />
Comparison between before and after, different locations, may be easier than to pin-point exactly what is producing the patterns.<br><br />
See one explanation at the bottom of this [http://www.biodynamics.in/chrom.htm webpage].<br />
<br><br />
The different colours we obtain has to be a reflection of the [http://www.saltlakemetals.com/Silver_Nitrate_Uses.htm chemical reaction with silver nitrate] and whatever compound that is deposited there. (It seems to be copied from [https://www.scrapmetalforum.com/scrap-metal-tips-advice/13866-silver-what-used-most-useful-all-noble-metals.html here])<br />
<br><br><br />
For Halides<br><br />
For example: <br><br />
* White=Chloride<br />
* Pale Yellow=Bromide<br />
* Yellow=Iodide.<br />
Test for the presence of Carbonate, Hydroxide, Sulfide and Phosphate ions.<br><br />
For example: <br><br />
* Pale Green=Carbonate<br />
* Brown=Hydroxide<br />
* Black=Sulfide.<br />
<br><br><br />
<br />
<br />
----<br />
<br />
*Bio Crystallisation, copper chloride crystallisation, proteins<br />
*Capillary Dynamolysis, vertical filters, ‘rising pictures’, sugars/ bitter materials<br />
*Bio Chromatography, horizontal circular images, minerals, sugars/ bitter materials<br />
<br />
#[http://www.biodynamic-research.net/ras/rm/pfm Description of methods]<br />
#[http://biodyn.se/en/bildskapande-forskningsmetoder/ Choosing appropriate methods]<br />
<br><br><br />
<br />
==Resources/Links==<br />
#[http://www.dynamis.tv/fr/cristallisation-sensible-morphochromatographie-biodynamie.php Chromatograpy of wine] <br />
#[http://www.levity.com/alchemy/kolisko.html Capillary Dynamolysis] <br />
#[http://biodyn.se/en/forskning-pagar/ Scandenavian Biodynamics Research] <br />
#[https://www.omicsgroup.org/journals/2165-7866/2165-7866-1-101.php?%252520aid=2429 Academic paper on Capillary Dynamolysis Image Discrimination Using Neural Networks] <br />
#[https://ledepotesta.wordpress.com/2016/01/20/koliskos-agriculture-of-tomorrow-pt-2/#more-133 Summary of Kolisko's book] <br />
#[http://www.sciencegroup.org.uk/history.htm History of Science Group of the Anthroposophical Society in Great Britain]<br />
#[https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/a/principles-of-chromatography Khan Academy Principles of Chromatography]<br />
#[http://karnet.up.wroc.pl/~weber/ekstrak2.htm Extraction of Soil Organic Matter]<br />
#[http://lab-training.com/2014/11/21/types-paper-chromatography/ Types of Paper Chromatography]<br />
[[Category:Analysis]][[Category:Environment]][[Category:Wetware]][[Category:Chemistry]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28411Slime Mould2018-06-08T13:34:20Z<p>Derishus: /* What is a protist? */</p>
<hr />
<div>[[File:Slime_mould_on_nutrient_agar_plate.JPG|Fuligo septica grown on nutrient agar plate|400px|right]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
A single cell eukaryote!<br><br />
The problem of classification of protists is described in this [https://youtu.be/Ln69k7LyTsU?t=1m51s video], which has movies of the diverse protista in motion under the microscope.<br><br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28410Slime Mould2018-06-08T13:27:48Z<p>Derishus: </p>
<hr />
<div>[[File:Slime_mould_on_nutrient_agar_plate.JPG|Fuligo septica grown on nutrient agar plate|400px|right]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
A single cell eukaryote!<br><br />
The problem of classification of protists is described in this [https://youtu.be/Ln69k7LyTsU?t=1m51s video].<br><br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28409Slime Mould2018-06-07T20:38:09Z<p>Derishus: </p>
<hr />
<div>[[File:Slime_mould_on_nutrient_agar_plate.JPG|Fuligo septica grown on nutrient agar plate|400px|right]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28408Slime Mould2018-06-07T20:37:52Z<p>Derishus: </p>
<hr />
<div>[[File:Slime_mould_on_nutrient_agar_plate.JPG|Fuligo septica grown on nutrient agar plate|200px|]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28407Slime Mould2018-06-07T20:37:32Z<p>Derishus: </p>
<hr />
<div>[[File:Slime_mould_on_nutrient_agar_plate.JPG|frame|Fuligo septica grown on nutrient agar plate|200px|]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28406Slime Mould2018-06-07T20:37:14Z<p>Derishus: </p>
<hr />
<div>[[File:Slime_mould_on_nutrient_agar_plate.JPG|frame|Fuligo septica grown on nutrient agar plate|400px|]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28405Slime Mould2018-06-07T20:36:46Z<p>Derishus: </p>
<hr />
<div>[[File:Slime_mould_on_nutrient_agar_plate.JPG|400px|frame|Fuligo septica grown on nutrient agar plate]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28404Slime Mould2018-06-07T20:36:26Z<p>Derishus: </p>
<hr />
<div>[[File:Slime_mould_on_nutrient_agar_plate.JPG|frame|Fuligo septica grown on nutrient agar plate]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=File:Slime_mould_on_nutrient_agar_plate.JPG&diff=28403File:Slime mould on nutrient agar plate.JPG2018-06-07T20:35:37Z<p>Derishus: </p>
<hr />
<div></div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28402Slime Mould2018-06-07T20:34:19Z<p>Derishus: </p>
<hr />
<div>[[File:example.jpg|frame|caption]]<br />
Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28401Slime Mould2018-06-07T20:33:20Z<p>Derishus: </p>
<hr />
<div>Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources<br />
* [http://www.buildingthepride.com/faculty/pgdavison/PHYSARUM%20culture%20for%20web.html A Simple Method of Growing the Plasmodial Slime Mold] written for Physarum polycephalum</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28400Slime Mould2018-06-07T16:01:05Z<p>Derishus: </p>
<hr />
<div>Slime Moulds have gotten a lot of attention of their ability to find optimised [https://youtu.be/GwKuFREOgmo transport maps], but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28399Slime Mould2018-06-07T15:59:33Z<p>Derishus: /* What is a protist? */</p>
<hr />
<div>Slime Moulds have gotten a lot of attention as a way to optimise transport maps, but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms commonly called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade]).<br />
# Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
# Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
# Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br><br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28398Slime Mould2018-06-07T15:58:39Z<p>Derishus: /* What is a protist? */</p>
<hr />
<div>Slime Moulds have gotten a lot of attention as a way to optimise transport maps, but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [http://www.ucmp.berkeley.edu/protista/slimemolds.html 3 groups] of organisms called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade])<br />
1. Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
2. Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
3. Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28397Slime Mould2018-06-07T15:58:08Z<p>Derishus: /* What is a Slime Mould? */</p>
<hr />
<div>Slime Moulds have gotten a lot of attention as a way to optimise transport maps, but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other. <br><br><br />
They are '''protists'''. <br><br />
===What is a protist?===<br />
There are [3 groups http://www.ucmp.berkeley.edu/protista/slimemolds.html] of organisms called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade])<br />
1. Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
2. Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
3. Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28396Slime Mould2018-06-07T15:57:53Z<p>Derishus: </p>
<hr />
<div>Slime Moulds have gotten a lot of attention as a way to optimise transport maps, but what are they???<br><br />
==What is a Slime Mould?==<br />
First they are NOT fungi - although for a long time, they were thought to be fungi because their life cycle resemble each other <br><br><br />
They are protists <br><br />
===What is a protist?===<br />
There are [3 groups http://www.ucmp.berkeley.edu/protista/slimemolds.html] of organisms called slime moulds, but they do not share a common ancestor ([https://en.wikipedia.org/wiki/Clade a clade])<br />
1. Plasmodial slime molds = giant cells, single cells with thousands of nuclei fused flagellated cells<br />
2. Cellular slime molds = mostly found as separate single-celled amoeboid protists, but can swarm with chemical signals<br />
3. Labyrinthulomycota, called slime moulds, but are not related to 1 and 2<br />
<br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28395Slime Mould2018-06-07T15:49:42Z<p>Derishus: </p>
<hr />
<div>Slime Moulds have gotten a lot of attention as a way to optimise transport maps, but what are they???<br><br />
<br />
First they are NOT fungi - <br><br />
They are protists <br><br />
==Resources==<br />
* [http://dictybase.org/ dictybase] where you can find genomes, protocols and other resources</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Slime_Mould&diff=28394Slime Mould2018-06-07T15:39:45Z<p>Derishus: Created page with "Slime Moulds have gotten a lot of attention as a way to optimise transport maps, but what are they???<br> First they are NOT fungi - <br> They are protists <br>"</p>
<hr />
<div>Slime Moulds have gotten a lot of attention as a way to optimise transport maps, but what are they???<br><br />
<br />
First they are NOT fungi - <br><br />
They are protists <br></div>Derishushttp://www.hackteria.org/wiki/index.php?title=Collection_of_DIY_Biology,_Open_Source_Art_Projects&diff=28393Collection of DIY Biology, Open Source Art Projects2018-06-07T15:37:15Z<p>Derishus: </p>
<hr />
<div><!-- Projects --><br />
<div class="alertBox1"><br />
{| class="alert"<br />
!Projects<br />
<br />
|-<br />
|'''Project 0: [[WetWare]]'''<br />
[[File:WetWare_s.jpg|120px|left]]<br />
Where to shop for materials<br />
<br />
|-<br />
|'''Project 1: [[The Bacterial Net- Microbial Telecommunications.]]'''<br />
[[Image:2.jpg|120px|left]]<br />
Quorum sensing is a type of decision-making process used by decentralized groups to coordinate behavior. Many species of bacteria use quorum sensing to coordinate their gene expression according to the local density of their population.The Bacterial Net attempts to transfer this decision making process over the Internet. Using Microcontollers and sensors to detect bacterial population and a public web service http://www.pachube.com that helps transfer sensor information. The Bacterial Net is an exploration in bringing the Internet into the Natural Ecosystem. <br />
<br />
|-<br />
|'''Project 2: [[Bioelectronix]]'''<br />
<br />
[[Image:bioelectronics_after_casting.jpg|120px|left]]<br />
<br />
Integrating electronics into biological culture devices needs different approaches. the use of silicone-rubbers for sealing off the wet/humid bio-part from the silicon circuits and sensors allows the making of hybrid bioelectronic devices. <br />
|-<br />
| '''Project 3: [[Synthetic Biology for Artists and Designers: A primer]]'''<br />
[[File:synbio_cover.jpg|120px|left]]<br />
An Illustrated [http://hackteria.org/wiki/images/a/a1/Handbook.pdf <strong>handbook</strong>] that shows you how to work with MIT's parts registry<br />
|-<br />
| '''Project 4: [[ArtScienceBangalore 2009-Synthetic Biology for Artists and Designers]]'''<br />
[[File:Whiteboard.jpg|120px|left]]<br />
<br />
see also the [http://prix2011.aec.at/winner/3128/ docu for Prix Ars Electronica]<br />
<br />
<br />
|-<br />
|'''Project 5: [[DIY microscopy]]'''<br />
[[File:cam_ani_sm.gif|left]]<br />
<br />
a small hack to the optics of a standard webcam with an adjustable focus-lens, allows to create video data, with a magnification of around 100 to 400x (depending on the model) at a working distance of a few mm. The highest magnifications can be achieved by inverting (putting upside down) the lens. Addition of good lighting by the use of leds allows to create images using a bright-field method (shine through the stuff) or dark field method (look at the reflections and scattering). <br />
<br />
|-<br />
|'''Project 6:[[DIY $25 Gel Box]]'''<br />
[[File:DSC 8050.jpg|120px|left]]<br />
Building your own Electrophoresis Gel Box is easy. Here's how you do it!<br />
<br />
|-<br />
| '''Project 7: [[bio habitats]]'''<br />
[[File:biohabitat.jpg|120px|left]]<br />
instead of using standard lab culture tools, such as flasks or petri-dishes, artists might want to design their own devices to culture and inhabitate living organisms and keep them alive. simple devices can be cast in silicone to incorporate all the necessary environemtal conditions for long time survival of microorganisms.<br />
<br />
|-<br />
| '''Project 8: [[DIY Microfluidics]]'''<br />
[[File:Microfluidicwikiimage.png|120px|left]]<br />
<br />
|-<br />
| '''Project 9: [[DIY Plant Tissue culture and Engineering]]'''<br />
[[File:plant_culture.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 10: [[Pd_microscope]]'''<br />
[[File:pd_microscope.png|120px|left]]<br />
Pd_microscope is the software package that you can use to control your DIY microscope. It's a work in progress<br />
<br />
|-<br />
| '''Project 11: [[DIY spectroscopy]]'''<br />
[[File:spektral_tag1.jpg|120px|left]]<br />
Spectroscopy means the analysis of natural phenomena according to its energy distribution. In case of the electromagnetic spectra, ranging from x-ray, visible light, infrated to radio frequencies, this can be used to analyse chemical compounds, follow biochemical reactions or get information of the composition of stars. This project tries utilize webcams, CD/DVD and some construction material, to get a spectral analysis of ultra-violett and visible light sources, which might be used to follow enzymatic and metabolic reactions in cultured microorganisms and bacteria.<br />
<br />
|-<br />
| '''Project 12: [[ArtScienceBangalore 2010-Synthetic Biology for Artists and Designers]]'''<br />
[[File:Asb_2010_group.jpg|120px|left]]<br />
A bunch of regular people with very little idea of Synthetic Biology but ready to pick up eveything about it.<br />
<br />
Download the nicely illustrated Comic Book on Synthetic Ecologies here [[File:IGEM_Comic_2010.pdf]]<br />
<br />
|-<br />
| '''Project 13: [[DIY handheld centrifuge]]'''<br />
[[File:HandHeldCent.jpg |120px|left]]<br />
<br />
|-<br />
| '''Project 14: [[DIY table top centrifuge]]'''<br />
[[File:Table_top.JPG|120px|left]]<br />
<br />
|-<br />
| '''Project 15: [[DIY Incubator]]'''<br />
[[File:DSC03096.JPG|120px|left]]<br />
<br />
<br />
|-<br />
| '''Project 16: [[DIY Fermentation or the Art of making Wine]]'''<br />
[[File:diy_fermentation.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 17: [[Hacked Optical Mouse]]'''<br />
[[File:optical_mouse_hack.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 18: [[DIY Sterlisation Hood]]'''<br />
[[File:DSC03946.JPG |120px|left]]<br />
<br />
|-<br />
| '''Project 19: [[DIY Water Bath]]'''<br />
[[File:DSC04040.JPG|120px|left]]<br />
<br />
|-<br />
| '''Project 20: [[GFP Protocol Jugaad]]'''<br />
[[File:MG_7283.JPG|120px|left]]<br />
Home made GFP bacteria<br />
<br />
|-<br />
| '''Project 21: [[ReacTable]]'''<br />
[[File:DSC04180.JPG|120px|left]]<br />
Gives information and protocals of equipment and bacteria put on it<br />
<br />
|-<br />
| '''Project 22: [[Algae Culture at Home]]'''<br />
[[File:Plankton.jpg|120px|left]]<br />
Grow your own algae at home with some plastic bottles, lighting and air bubbles<br />
<br />
|-<br />
| '''Project 23: [[Mobile Phone Field Microscope]]'''<br />
[[File:hacked_phone.jpg|120px|left]]<br />
Modify a mobile phone into a useful field microscope by replacing the lens with a cheap flipped wemcam lens..<br />
<br />
|-<br />
| '''Project 24: [[DIY turbidity meters]]'''<br />
[[File:turbidity_shield.jpg|120px|left]]<br />
simple light absorption measurements for water monitoring or nanoparticle detection using an arduino, a laser or LED and an LDR.<br />
<br />
|-<br />
| '''Project 25: [[Laser-cut microscopy stages]]'''<br />
[[File:miniScope.jpg|120px|left]]<br />
different design for easy to build stages for the DIY microscope, z-stage, x-y servo controlled stage and more...<br />
<br />
|-<br />
| '''Project 26: [[How to start a nanolab]]'''<br />
[[File:nanolab.jpg|120px|left]]<br />
Because nanotechnology is situated at the interface of many disciplines it is best to organize some basic gear from various sources, such as a local electronics store, gardening shops or various online suppliers for household tools and electronic gadgets. You will need to set up some basic laboratory instruments for doing simple biology, chemistry, fabrication&manufacturing and some physical computing to connect everything to your computer.<br />
<br />
|-<br />
| '''Project 27: [[Magnetic stirrer]]'''<br />
[[File:NS_spinner_spinning.jpg|120px|left]]<br />
always useful to stir things automatically if you are lazy...<br />
<br />
|-<br />
|'''Project 28: [[DIY Micro Dispensing and Bio Printing]]'''<br />
[[File:nanofab.jpg|120px|left]]<br />
dispensing, cutting, printing, growing with a robotic platform. Aka NanoFabrik<br />
<br />
|-<br />
|'''Project 29: [[ArtScienceBangalore 2011-Synthetic Biology for Artists and Designers]]'''<br />
[[File:2011artsciblr.jpg|120px|left]]<br />
[http://2011.igem.org/Team:ArtScienceBangalore Link to IGEM site]<br />
<br />
Download the nicely illustrated booklet on "Dirt" here [[File:dirt_IGEM2011.pdf]]<br />
<br />
|-<br />
|'''Project 30: [[ArtScienceBangalore 2011-Nanotechnology for Artists and Designers]]'''<br />
[[File:Mindmap1.JPG|120px|left]]<br />
[http://openwetware.org/wiki/Biomod/2011/SRISHTI/ArtScienceBangalore Link to Biomod site]<br />
<br />
|-<br />
|'''Project 31: [[TEMPE(h) : THE INSTRUCTIONS]]'''<br />
[[File:tempe3.jpg|120px|left]]<br />
Tempe(h) is a fermented food. But it's not meant to preserve the material from rottenness. It's for style. Analogue meat they said. So, once you made your own tempe(h). Cook it! Eat it! <br />
<br />
<br />
<br />
|-<br />
|'''Project 32: [[Daphniaology]]'''<br />
[[File:daphnia_jar.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 33: [[Ants]]'''<br />
[[File:and_lenoir.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 34: [[Book: Homemade Biological Art]]'''<br />
[[File:homemade_BioArt.jpg|120px|left]]<br />
<br />
|-<br />
|'''Project 35: [[The Art of Hunting]]'''<br />
[[File:hunter.jpg|120px|left]]<br />
Baerman funnels, Berlese-Tullgren funnel, habitats<br />
<br />
|-<br />
|'''Project 36: [[Jugaad PCR Thermocyclers]]'''<br />
[[File:Hairdryer_PCR_lab2011.jpg|120px|left]]<br />
some links to alternative and DIY thermocycling experiments.<br />
<br />
|-<br />
|'''Project 37: [[MobileLabs]]'''<br />
[[File:mobilelabs.jpg|120px|left]]<br />
some links and ideas to mobile labs...<br />
<br />
|-<br />
|'''Project 38: [[Autonomous Public Lab]]'''<br />
[[File:Buildingabiodome.jpg|120px|left]]<br />
Blueprints for setting up a People's Research Lab<br />
<br />
|-<br />
|'''Project 39: [[Hackteria Merchandise and Logos]]'''<br />
[[File:Biohacker.png|120px|left]]<br />
loads of logos, t-shirt designs, stickers, flyers and posters<br />
<br />
|-<br />
|'''Project 40: [[Orbital Shaker]]'''<br />
[[File:orbital_shaker.jpg|120px|left]]<br />
shake it baby!<br />
<br />
|-<br />
|'''Project 41: [[Plant Electrophysiology]]'''<br />
[[File:web9.jpg|120px|left]]<br />
Yeah, even plants cant be protected from arduinos..<br />
<br />
|-<br />
|'''Project 42: [[Spin Coater]]'''<br />
[[File:spin_coater.jpg|120px|left]]<br />
DIY Spin Coater!<br />
<br />
|-<br />
|'''Project 43: [[NeuroNetworking]]'''<br />
[[File:dkbrain.jpg|120px|left]]<br />
Prototypes & design fiction into networking over brain data and biodata related to our brain function (fMRI, SNPs, EEG).<br />
How will we use brain data for social networking, neuromarketing, citizen science projects,etc.<br />
The main technical challenge is to create some open format for sharing brain data over the web (3D T1-weighted gradient-echo sequence (MP-RAGE)images in the nifti format http://nifti.nimh.nih.gov/nifti-1 , which is readable by FSL software (http://www.fmrib.ox.ac.uk/fsl/ into some 3D web browser data and image that would support crowdsourcing of brain data, pattern recognition and comparison of brain data).<br />
<br />
|-<br />
|'''Project 44: [[Garden 2012: Spherification of Hieronymus Bosch]]'''<br />
[[File:Spheres_big.jpg|120px|left]]<br />
Spherification experiments as a tribute to Hieronymus Bosch's Garden of Earthly Delights. In order to understand our new cosmology of minimal but also complex spheres (molecules, cells, vesicles, coacervates, microspheres), we decided to use spherification techniques and to try to design a version of Bosch's Garden of Earthly Delights like a catalogue of what is possible in terms of spheres today. Some of our spheres are eatable, other are perceived only by microscopes, some target taste buds other just molecules, we even tried to prepare a micro-meal for microorganisms entrapped in a type of mini-aquaria and systems. The three containers mimicking the original triptych are an early reflection of the importance of spheres for our idea of origin or the organic life but also present experiments in molecular gastronomy but also drug targeting systems and research in nano-bio technologies. Humans making spheres on all scales are like gods recreating some new garden with self-enclosed systems, ecologies and microspheres, which influence each other and interact.<br />
<br />
|-<br />
|'''Project 45: [[Bio+Hacker+Art-Space Tour California 2012]]'''<br />
[[File:cal_local.jpg|120px|left]]<br />
Picture protocoll of an intensive three week travel throug the venues of third culture in western California!<br />
<br />
<br />
|-<br />
|'''Project 46: [[Distillation]]'''<br />
[[File:distillation.jpg|120px|left]]<br />
Distillation at the Hackteria Distillery at Jagaa, Bangalore Dec. 2012 and other distillation rigs<br />
<br />
|-<br />
|'''Project 47: [[Do eat your laser Tofu (DEYLT)]]'''<br />
[[File:tof_icon.jpg|120px|left]]<br />
How to make your own tofu? From laser cutting a tofu mold to the preparation and the tasting.<br />
<br />
|-<br />
|'''Project 48: [[Randen-Burger or the art of documenting]]'''<br />
[[File:randen.jpg|120px|left]]<br />
...<br />
<br />
|-<br />
|'''Project 49: [[Hackteria_%26_SGMK_BioCyberKidzz#Yeast_powered_balloons|Yeast powered balloons a la BioCyberKidzz]]'''<br />
[[File:Maxiblasinator.jpg|120px|left]]<br />
This is a balloon, which inflates as a result of the yeast growing and interacting with its environment <br />
<br />
|-<br />
|'''Project 50: [[Hackteria_%26_SGMK_BioCyberKidzz#Bacterial_jewelery|Bacterial Jewelry a la BioCyberKidzz]]'''<br />
[[File:jewelry.jpg|120px|left]]<br />
The Bacterial Jewelry module comes as a take away amulet that has a living fingerprint of the participant. <br />
<br />
<br />
|-<br />
|'''Project 51: [[Hackteria_%26_SGMK_BioCyberKidzz#Body_Augmentation_and_Enhancement|Body Augmentation and Enhancement a la BioCyberKidzz]]'''<br />
[[File:elephantear.jpg|120px|left]]<br />
Talking about bioart and cyberbody modifications is accessible to everyone. <br />
<br />
|-<br />
|'''Project 52: [[Hackteria_%26_SGMK_BioCyberKidzz#LabBook|The LAB BOOK a la BioCyberKidzz]]'''<br />
[[File:laborbuch2.jpg|120px|left]]<br />
Recording observations is an important aspect of laboratory life. This module is to encourage the collection and recording of data. <br />
<br />
|-<br />
|'''Project 53: [[DIY Laser tweezer, cell trap, oligo synthesis]]'''<br />
[[File:tweez.png|120px|left]]<br />
Based on a paper of a laser tweezer made from an old DVD drive we go on...<br />
<br />
<br />
|-<br />
|'''Project 54: [[DIY Plasma generators]]'''<br />
[[File:plasma_siech.jpg|120px|left]]<br />
Where to get it, how to make it, the 4th state of matter.<br />
<br />
|-<br />
|'''Project 55: [[Hacked Hard-disc Centrifuge]]'''<br />
[[File:harddisc_centrifuge.jpg|120px|left]]<br />
Get an old harddisc, laser cut or 3D pring a eppi-holder, run it...<br />
<br />
|-<br />
|'''Project 56: [[Agar is the Media]]'''<br />
[[File:agar_media.jpg|120px|left]]<br />
The key technologies of every genetic and molecular biology lab – the gel electrophoresis chamber...<br />
<br />
<br />
|-<br />
|'''Project 57: [[BubbleBots]]'''<br />
[[File:BubbleBots.jpg|120px|left]]<br />
Tiny little tubular rockets generating bubbles to propel themselves through water.<br />
<br />
|-<br />
|'''Project 58: [[Ganzfeld experiment]]'''<br />
[[File:Ganzfeld experiment.JPG|120px|left]]<br />
This is a modified experiment from the 70s and which can be used as a great icebreaker activity for building trust.<br />
<br />
|-<br />
|'''Project 59: [[Rubber_Hand_Illusion_Experiment_variations]]'''<br />
[[File:Wooden-hand.jpeg|120px|left]]<br />
a variation developed through trying this workshop on a large number of people, and not having funding to get loads of rubber hands. So we made the hands in the workshop.<br />
<br />
|-<br />
|'''Project 60: [[Internet of Bugs'n'Beasts]]'''<br />
[[File:bugsnbug.jpeg|120px|left]]<br />
starting soon<br />
<br />
|-<br />
|'''Project 61: [[KIMCHI or the Art of Fermentation]]'''<br />
[[File:kimchi01.jpg|120px|left]]<br />
HomeMade KIMCHI from scratch.....<br />
The final taste all depends on the amount of salt, dehydration, fermentation and temperature monitor.<br />
<br />
<br />
<br />
|-<br />
|'''Project 62: [[Science For Poets]]'''<br />
[[File:Webbana.jpg|120px|left]]<br />
LifeSciences Workshop for Artists & Designers in Bangalore 2013, collaborators of biodesign.cc<br />
<br />
|-<br />
|'''Project 63: [[Zebrafish Hacking: The Plant Animal]]'''<br />
[[File:ZEBRAFISH.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 64: [[Wild OpenPCR]]'''<br />
[[File:WildOpenPCR.JPG|120px|left]]<br />
Building an OpenPCR without the kit, just from commercially available parts. With additional information on how to do it.<br />
<br />
<br />
|-<br />
|'''Project 65: [[Explorations in BioLuminescence]]'''<br />
[[File:bioluminescence.jpg|120px|left]]<br />
From fireflies to stinky squids... the future is bright<br />
<br />
<br />
|-<br />
|'''Project 66: [[Say Cheese]]'''<br />
[[File:Cheesemaking.jpg|120px|left]]<br />
make your own fresh cheese<br />
<br />
<br />
|-<br />
|'''Project 67: [[Hack a Taq]]'''<br />
[[File:Hack-a-taq.jpg|120px|left]]<br />
Testing out the Wild OpenPCR and purifying your own Taq from Open Biotechnology - using primers to detect coliform bacteria <br />
<br />
|-<br />
|'''Project 68: [[Poor Man's BioPrinter]]'''<br />
[[File:poorBioPrinter.jpg|120px|left]]<br />
There are many ways of bioprinting...<br />
<br />
|-<br />
|'''Project 69: [[DIYbio Prototypes Collection & Hackteria archive]]'''<br />
[[Image:2.jpg|120px|left]]<br />
Collection of open biology and DIYbio prototypes created by members of the Hackteria network, such as DNA Lab-in-Box, Gene gun etc. This archive of exhibition artefacts supports open science and citizen science advocacy and showcases the open hardware possibilities of supporting research in the Global South.<br />
<br />
|-<br />
|'''Project 70: [[Urban Cuisine]]'''<br />
[[Image:snail_huntin.jpg|120px|left]]<br />
Various explorations into urban ecologies and recipes to hunt, collect, grow, cook, eat your findings <br />
<br />
|-<br />
|'''Project 71: [[Actinomycetes Tournament: Open antibiotics discovery]]'''<br />
[[Image:acti.jpg|120px|left]]<br />
In this project we want to screen soil bacteria, such as Actinomyces, Streptomyces and Micromonospora for antibiotic properties. It is a global citizen science initiative started by DIYbio Waag, which uses game elements (collecting and tournaments) to support open drug discovery of antibiotics. We will isolate actinomycetes from soil samples around the world and screen with Micrococcus luteus or similar microbe to determine their antibiotic properties. Images of inoculated plates will be shared online and based on their strengths (zones of growth inhibition) they will become part of a collection or initiate tournaments between the most successful examples. We will sequence the most powerful ones. We expect cooperation with various DIYbio organizations, microbiology departments and science enthusiasts. <br />
Picture: Antibiotic Producing Actinomycetes By adonofrio (Biology101.org)<br />
<br />
|-<br />
|'''Project 72: [[High Voltage Epigenetics]]'''<br />
[[Image:high_voltage_yeast.jpg|120px|left]]<br />
Is it real or is it epigenetics? A novel method is described, which, on the basis of the short-term application of electrostatic fields, results in lasting beneficial and desirable properties in animals and plants. The culture of fish, particularly of edible fish but also of ornamental fish, is genuinely revolutionised. <br />
<br />
|-<br />
|'''Project 73: [[How to make a proper Barber Trap]]'''<br />
[[Image:BarberTrap.png|120px|left]]<br />
<br />
|-<br />
|'''Project 74: [[doms Mobile vj lab]]'''<br />
As time past I have grow huge need for mobile vj lab for all indoor and outdoor visual activity.<br />
From my experience till now it has to be paced with lots of power, projector, cams, microscope,sound system, bio lab tools (pipettes, slide glass...),many different vj tools(blinking led...), lasers, traps and containers for live organisms. <br />
Probably some other stuff that I will find useful like knife, glue, bugs 2d aquarium, different plugs for charging and powering<br />
that you always find helpful on terrain.<br />
Of course all together should not be to heavy (5kg max but rather 3kg)....<br />
... <br />
<br />
|-<br />
|'''Project 75: [[openTrap]]'''<br />
[[Image:FoxTrap.jpg|120px|left]]<br />
More and more we are looking into various traps for catching larger animals such as rats, foxes, pigeons and more. Re-developing some popular designs, adapting them, make them open source for digital fabrication<br />
... <br />
<br />
<br />
|-<br />
|'''Project 76: [[DIY_NanoDrop]]'''<br />
[[Image:DIYNanoDrop.jpg|120px|left]]<br />
Microvolume Spectrophotometers and Fluorospectrometers are innovative products used to quantitate micro-volumes of sample. You want one? Build your own.<br />
... <br />
<br />
<br />
|-<br />
|'''Project 77: [[Nail Studio Micro Fluidics]]'''<br />
[[Image:NailStudioMicroFluidics.jpg|120px|left]]<br />
Nail studios are the girls secret hight tech labs with UV light curing gel and other fancy stuff. Let's hack it to do low cost microfluidics.<br />
... <br />
<br />
|-<br />
|'''Project 78: [[Mobile Power Solutions]]'''<br />
[[Image:PowerSolutions.jpg|120px|left]]<br />
You need mobile electrical power - On how to build Graphene Supercapacitors, Peltier electricity from fire and more...<br />
<br />
|-<br />
|'''Project 79: [[Fish-Hacking]]'''<br />
[[Image:IMG_2593.JPG|120px|left]]<br />
Ever tried to listen into the electrocommunication behavior of weakly electric fish? Recent experiments and general info about black ghost knifefish, inspired by [http://hackteria.org/wiki/index.php/Fish-to-Brain_Interfaces,_Antony_Hall,_Ljubljana Tony Hall's work on ENKI technology]. They have also been shown to be useful for biomonitoring of pullutants in water! Fish-2-pd interfaces are already working nicely.<br />
<br />
|-<br />
|'''Project 80: [[Long Range Microphone for Cicadas & Cricket Hunting]]'''<br />
[[Image:Garengpung.jpg|120px|left]]<br />
Imagine you are in the forest, rivers, or mountains and some noises just appears and hunting your ears and you kind of wanted to be sure what is it and where is it come from. This long range microphone might help you to solve the mistery. But, as the title says it will be used for hunting cricket and cicadas a.k.a garengpung, then we must have some ideas about how the cricket and cicadas a.k.a garengpung sounds like. - still under construction. Hhaha... so is everything else on that wiki :-)<br />
<br />
|-<br />
|'''Project 81: [[DIY Microbial Fuel Cells]]'''<br />
[[Image:Mud_Battery.png|120px|left]]<br />
Imagine you are in the forest and create a bit of electricity from mud...<br />
<br />
|-<br />
|'''Project 82: [[Water Sampling Coconut]]'''<br />
[[Image:CoconutSensor.jpg|120px|left]]<br />
GPS and water sensors in a coconut go explore the rivers.<br />
<br />
|-<br />
|'''Project 83: [[Psychokinetic Action of Young Chicks]]'''<br />
[[Image:PsychoCkicks.jpg|120px|left]]<br />
What if there was such a thing as Psychokinetics - let's test. <br />
<br />
|-<br />
|'''Project 84: [[Aquatic Malaria Extinction Robot - TEAM1]]'''<br />
[[Image:Malaria_siech_dusjagr.jpg|120px|left]]<br />
Some thoughts and experiments about malaria reduction, mosquito killing and stinky socks<br />
<br />
|-<br />
|'''Project 85: [[SATW - Do-it-yourself von Laborgeräten in der Bioanalytik]]'''<br />
[[Image:SATW-DIY_kit.jpg|120px|left]]<br />
Co-Development of a workshop on DIY laboratory instruments for bioanalytics, colorimetry, low-cost microcontrollers and simple DIY electronics, enzymatic assays. With a concept of "teach the teachers", we are looking into DIY lab-tools as means of a pedagogic tool for interdisciplinary thinking at the biomedical/engineering/molecular interface. Coproduced with SATW, Swiss Academy of Technical Sciences, Hackteria and FHNW, School for Lifesciences.<br />
<br />
|-<br />
|'''Project 86: [[GynePUNK biolabs]]'''<br />
[[Image:smell_my_finger.jpg|120px|left]]<br />
key words: ANARCHA biopunk - DIY bodyaction - radical SelFpower - Analisis of fluids - self citology - DIY equipment<br />
<br />
BIO-reSEARCH is part of [[Pechblenda]] tentacles, mixed with [http://anarchagland.hotglue.me AnarchaGland] researchs and [http://gynepunk.tumblr.com/ GynePUNK biolabs]<br />
<br />
|-<br />
|'''Project 87: [[Ayam Cemani - ... or the Black Chicken Project]]'''<br />
[[Image:Ayam cemani kid.jpg|120px|left]]<br />
Ayam means "chicken" in Indonesian and Cemani is "completely black" in Javanese.<br />
<br />
|-<br />
|'''Project 88: [[Lick the Moonmilk]]'''<br />
[[Image:Mondmlichloch.png|120px|left]]<br />
Exploring the origin and use of moonmilk. Cave safari to the Moondmilchloch in Lucern.<br />
<br />
|-<br />
|'''Project 89: [[Elektrowetting]]'''<br />
[[Image:Elektrowetting.png|120px|left]]<br />
Playing with electro-wetting effect. Trying to finally make that desktop DNA synthesizer.<br />
<br />
|-<br />
|'''Project 90: [[Nucleic_Acid_Quantitation]]'''<br />
[[Image:nucleicAcidAbsorbtion.png|120px|left]]<br />
Nucleic_Acid_Quantitation<br />
<br />
|-<br />
|'''Project 91: [[Wild openQCM]]'''<br />
[[Image:wild_openQCM.jpg|120px|left]]<br />
Make your free open wild openQCM aka artificial nose<br />
<br />
|-<br />
|'''Project 92: [[CocoMake7]]'''<br />
[[Image:CocoMake7_boardShape.png|120px|left]]<br />
A jugaad ultra low-cost clone to the Makey Makey, but based on QTouchADC sensing, local manufacturing and coconuts<br />
<br />
|-<br />
|'''Project 93: [[Nano hacking science and DIY/DIWO low cost diagnosis]]'''<br />
[[Image:IMG_0845.png|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 94: [[MicroMacroPleasure]]'''<br />
[[Image:Ursspeculum.jpg|120px|left]]<br />
Dildomancy hacking science and DIY/DIWO low cost pleasure and vaginal trainning<br />
<br />
|-<br />
|'''Project 95: [[Biocomons.Lab]]'''<br />
[[Image:11738011_692237857544097_3209041620878190282_n.jpg|120px|left]]<br />
A meeting to define the bio-value and the bio-capital in the era of biotechnology. 6 days of unconferences and workshops to push further the [http://www.bio-commons.org/ Bio-Commons research project].<br />
<br />
|-<br />
|'''Project 96: [[Mobile Plant Analysis Lab @ pifCamp, Slovenia]]'''<br />
[[Image:DusjagrLab_pifCamp.jpg|120px|left]]<br />
PIFcamp - Summer Hacking Camp, 3 – 9 August 2015 @ Trenta, Slovenia<br />
<br />
|-<br />
|'''Project 97: [[Jugaad PhoneScope]]'''<br />
[[Image:Lens_closeUp.jpg|120px|left]]<br />
Very simple and self-made DIY lens to attach to your smart-phone and turning it into a nice microscope.<br />
<br />
|-<br />
|'''Project 98: [[openlab gynecologyDIT]]'''<br />
[[Image:Dr-harry-waites-vibrator.jpg|120px|left]]<br />
Residence Fablab PlateformeC [www.plateforme-c.org] One of the topics or lines to explore Antropocene Or not ! PLASTICOCENE<br />
<br />
|-<br />
|'''Project 99: [[Euglena Burger]]'''<br />
[[Image:japan_euglenaBurger.png|120px|left]]<br />
The Euglena Burger - half animal and half plant. finally working on the recipes, upscaling using custom made home bio-reactors and looking forward to serve it to our friends.<br />
<br />
|-<br />
|'''Project 100: [[Save the Whales]]'''<br />
[[Image:pandas_will_save_the_whales.jpg|120px|left]]<br />
No more words - We need ACTION!<br />
<br />
|-<br />
|'''Project 101: [[PCR your DNA Workshop]]'''<br />
[[Image:WildOpenPCR.jpg|120px|left]]<br />
All you need to prepare for a basic workshop on getting some DNA, setting up a PCR, and running the gel to see some results in a day-long workshop<br />
<br />
|-<br />
|'''Project 102: [[Worm Composting]]'''<br />
[[Image:Wormcompost_3months.jpeg|120px|left]]<br />
Indoors or outdoors, a great way to reduce your garbage amounts and get super-rich compost for the garden<br />
<br />
|-<br />
|'''Project 103: [[Open Source Estrogen]]'''<br />
[[File:PIIS001078241100179X.gr1.lrg.jpg|120px|left]]<br />
Community page for DIY estrogen!!!<br />
<br />
|-<br />
|'''Project 104: [[Feuerphilosophen]]'''<br />
[[File:Alchemy.jpg|120px|left]]<br />
Alchemy Reenactment Session<br />
<br />
<br />
|-<br />
|'''Project 105: [[Fostering Duckweed]]'''<br />
[[File:DSC01035.jpg|120px|left]]<br />
Duckweed might be the food of the future? Can we grow it from our own pee as fertilizer?<br />
<br />
<br />
|-<br />
|'''Project 106: [[Völlig Übertriibe]]'''<br />
[[File:Mark_aquarium.jpg|120px|left]]<br />
Session in Schaffhausen for exploring BioVJing and other crazy Hydra-hacks<br />
<br />
<br />
|-<br />
|'''Project 107: [[Laser-Technologie 2000]]'''<br />
[[File:LaserTechnologie_2000_circuit.png|120px|left]]<br />
simple constant current circuit for hacking lasers<br />
<br />
<br />
|-<br />
|'''Project 109: [[Chrisper-Chäsli]]'''<br />
[[File:DIY_CRISPR_Kits.jpg|120px|left]]<br />
Some reflections on gene-editing and making cheese<br />
<br />
<br />
|-<br />
|'''Project 110: [[PRG Ikan Hobi - GMO fish geeking]]'''<br />
[[File:PRK_ikan_Jan2017_lifepatch.jpg|120px|left]]<br />
Geeking around with zebra-fish, CRISPR and the GM-fish (formerly know as glow-fish) we found on the market in Yogyakarta. Seems the Indonesian regulations are supporting the use of GM fish for sports, hobby, crafts and ornamental use!<br />
<br />
<br />
|-<br />
|'''Project 111: [[DIY chemistry analysis of food]]'''<br />
[[File:CuvettesGrapesmall.jpg|120px|left]]<br />
Series of protocols in development for extracting and analysing micronutrients in foods, using DIY methods and without the need for lab access.<br />
<br />
<br />
|-<br />
|'''Project 112: [[Make you own CRISPR-Kit]]'''<br />
[[File:TheCRISPR.jpg|120px|left]]<br />
Creative workshop at Schloss Solitude. Design your own CRISPR-Kit.<br />
<br />
|-<br />
|'''Project 112: [[Miso]]'''<br />
[[File:Dplus_miso_mixSojaRice.jpg|120px|left]]<br />
Miso (味噌 or みそ) making Workshop at Dimension Plus TW<br />
<br />
|-<br />
|'''Project 113: [[DIY Single Photon Generator]]'''<br />
[[File:SinglePhoton.jpg|120px|left]]<br />
Finding a hack to generate single photons. Quantum-Nano-Dots, Cristals, Ions?<br />
<br />
|-<br />
|'''Project 114: [[Gär Lämpli]]'''<br />
[[File:GarLampli_box_Pink.jpg|120px|left]]<br />
Check the Temperature and Time profile of your ferments... with just 1 LED<br />
<br />
|-<br />
|'''Project 115: [[Back to Bombyx]]'''<br />
[[File:LiftOfSpiderPrint.JPG|120px|left]]<br />
Exploring silk, again.<br />
<br />
|-<br />
|'''Project 116: [[Circular Chromatography]]'''<br />
[[File:Circular_chromatography.jpg|120px|left]]<br />
Started at [http://wiki.artscienceblr.org/wiki/index.php/Circular_chromatography (Art)ScienceBLR's site], enjoy concentric separation of matter - from soil, and developing sugars<br />
<br />
|-<br />
|'''Project 117: [[DIY Mutagenesis]]'''<br />
[[File:Mutagenesis.png|120px|left]]<br />
à la ur-institute<br />
<br />
<br />
|-<br />
|'''Project 118: [[Trans Organs on a Chip]]'''<br />
[[File:Transorgans_v1.png|120px|left]]<br />
grow your own transorgans for fully (micro) fluid(ic) exchange!<br />
<br />
|-<br />
|'''Project 119: [[Participation in Decision Making by Members of DIY Communites]]'''<br />
[[File:39890639411_d41caf1935_k.jpg|120px|left]]<br />
Research project on the needs of the community for sucessful collaboration with institutions<br />
<br />
<br />
|-<br />
|'''Project 120: [[HiSeq2000_-_Next_Level_Hacking]]'''<br />
[[File:HiSeq.jpg|120px|left]]<br />
Let's hack that HiSeq Next Level Sequencing machine and make it open!<br />
<br />
|-<br />
|'''Project 121: [[MakeBlock Me High-Power Encoder Motor Driver]]'''<br />
[[File:12045_01-500x500.JPG|120px|left]]<br />
Open Leaking Stuff<br />
<br />
|-<br />
|'''Project 122: [[Slime Mould]]'''<br />
[[File:Slime_mould_in_situ.JPG|120px|left]]<br />
more than solving the traveling salesman's problem<br />
<br />
<br />
<!-- Projects end --></div>Derishushttp://www.hackteria.org/wiki/index.php?title=Collection_of_DIY_Biology,_Open_Source_Art_Projects&diff=28392Collection of DIY Biology, Open Source Art Projects2018-06-07T15:33:54Z<p>Derishus: </p>
<hr />
<div><!-- Projects --><br />
<div class="alertBox1"><br />
{| class="alert"<br />
!Projects<br />
<br />
|-<br />
|'''Project 0: [[WetWare]]'''<br />
[[File:WetWare_s.jpg|120px|left]]<br />
Where to shop for materials<br />
<br />
|-<br />
|'''Project 1: [[The Bacterial Net- Microbial Telecommunications.]]'''<br />
[[Image:2.jpg|120px|left]]<br />
Quorum sensing is a type of decision-making process used by decentralized groups to coordinate behavior. Many species of bacteria use quorum sensing to coordinate their gene expression according to the local density of their population.The Bacterial Net attempts to transfer this decision making process over the Internet. Using Microcontollers and sensors to detect bacterial population and a public web service http://www.pachube.com that helps transfer sensor information. The Bacterial Net is an exploration in bringing the Internet into the Natural Ecosystem. <br />
<br />
|-<br />
|'''Project 2: [[Bioelectronix]]'''<br />
<br />
[[Image:bioelectronics_after_casting.jpg|120px|left]]<br />
<br />
Integrating electronics into biological culture devices needs different approaches. the use of silicone-rubbers for sealing off the wet/humid bio-part from the silicon circuits and sensors allows the making of hybrid bioelectronic devices. <br />
|-<br />
| '''Project 3: [[Synthetic Biology for Artists and Designers: A primer]]'''<br />
[[File:synbio_cover.jpg|120px|left]]<br />
An Illustrated [http://hackteria.org/wiki/images/a/a1/Handbook.pdf <strong>handbook</strong>] that shows you how to work with MIT's parts registry<br />
|-<br />
| '''Project 4: [[ArtScienceBangalore 2009-Synthetic Biology for Artists and Designers]]'''<br />
[[File:Whiteboard.jpg|120px|left]]<br />
<br />
see also the [http://prix2011.aec.at/winner/3128/ docu for Prix Ars Electronica]<br />
<br />
<br />
|-<br />
|'''Project 5: [[DIY microscopy]]'''<br />
[[File:cam_ani_sm.gif|left]]<br />
<br />
a small hack to the optics of a standard webcam with an adjustable focus-lens, allows to create video data, with a magnification of around 100 to 400x (depending on the model) at a working distance of a few mm. The highest magnifications can be achieved by inverting (putting upside down) the lens. Addition of good lighting by the use of leds allows to create images using a bright-field method (shine through the stuff) or dark field method (look at the reflections and scattering). <br />
<br />
|-<br />
|'''Project 6:[[DIY $25 Gel Box]]'''<br />
[[File:DSC 8050.jpg|120px|left]]<br />
Building your own Electrophoresis Gel Box is easy. Here's how you do it!<br />
<br />
|-<br />
| '''Project 7: [[bio habitats]]'''<br />
[[File:biohabitat.jpg|120px|left]]<br />
instead of using standard lab culture tools, such as flasks or petri-dishes, artists might want to design their own devices to culture and inhabitate living organisms and keep them alive. simple devices can be cast in silicone to incorporate all the necessary environemtal conditions for long time survival of microorganisms.<br />
<br />
|-<br />
| '''Project 8: [[DIY Microfluidics]]'''<br />
[[File:Microfluidicwikiimage.png|120px|left]]<br />
<br />
|-<br />
| '''Project 9: [[DIY Plant Tissue culture and Engineering]]'''<br />
[[File:plant_culture.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 10: [[Pd_microscope]]'''<br />
[[File:pd_microscope.png|120px|left]]<br />
Pd_microscope is the software package that you can use to control your DIY microscope. It's a work in progress<br />
<br />
|-<br />
| '''Project 11: [[DIY spectroscopy]]'''<br />
[[File:spektral_tag1.jpg|120px|left]]<br />
Spectroscopy means the analysis of natural phenomena according to its energy distribution. In case of the electromagnetic spectra, ranging from x-ray, visible light, infrated to radio frequencies, this can be used to analyse chemical compounds, follow biochemical reactions or get information of the composition of stars. This project tries utilize webcams, CD/DVD and some construction material, to get a spectral analysis of ultra-violett and visible light sources, which might be used to follow enzymatic and metabolic reactions in cultured microorganisms and bacteria.<br />
<br />
|-<br />
| '''Project 12: [[ArtScienceBangalore 2010-Synthetic Biology for Artists and Designers]]'''<br />
[[File:Asb_2010_group.jpg|120px|left]]<br />
A bunch of regular people with very little idea of Synthetic Biology but ready to pick up eveything about it.<br />
<br />
Download the nicely illustrated Comic Book on Synthetic Ecologies here [[File:IGEM_Comic_2010.pdf]]<br />
<br />
|-<br />
| '''Project 13: [[DIY handheld centrifuge]]'''<br />
[[File:HandHeldCent.jpg |120px|left]]<br />
<br />
|-<br />
| '''Project 14: [[DIY table top centrifuge]]'''<br />
[[File:Table_top.JPG|120px|left]]<br />
<br />
|-<br />
| '''Project 15: [[DIY Incubator]]'''<br />
[[File:DSC03096.JPG|120px|left]]<br />
<br />
<br />
|-<br />
| '''Project 16: [[DIY Fermentation or the Art of making Wine]]'''<br />
[[File:diy_fermentation.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 17: [[Hacked Optical Mouse]]'''<br />
[[File:optical_mouse_hack.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 18: [[DIY Sterlisation Hood]]'''<br />
[[File:DSC03946.JPG |120px|left]]<br />
<br />
|-<br />
| '''Project 19: [[DIY Water Bath]]'''<br />
[[File:DSC04040.JPG|120px|left]]<br />
<br />
|-<br />
| '''Project 20: [[GFP Protocol Jugaad]]'''<br />
[[File:MG_7283.JPG|120px|left]]<br />
Home made GFP bacteria<br />
<br />
|-<br />
| '''Project 21: [[ReacTable]]'''<br />
[[File:DSC04180.JPG|120px|left]]<br />
Gives information and protocals of equipment and bacteria put on it<br />
<br />
|-<br />
| '''Project 22: [[Algae Culture at Home]]'''<br />
[[File:Plankton.jpg|120px|left]]<br />
Grow your own algae at home with some plastic bottles, lighting and air bubbles<br />
<br />
|-<br />
| '''Project 23: [[Mobile Phone Field Microscope]]'''<br />
[[File:hacked_phone.jpg|120px|left]]<br />
Modify a mobile phone into a useful field microscope by replacing the lens with a cheap flipped wemcam lens..<br />
<br />
|-<br />
| '''Project 24: [[DIY turbidity meters]]'''<br />
[[File:turbidity_shield.jpg|120px|left]]<br />
simple light absorption measurements for water monitoring or nanoparticle detection using an arduino, a laser or LED and an LDR.<br />
<br />
|-<br />
| '''Project 25: [[Laser-cut microscopy stages]]'''<br />
[[File:miniScope.jpg|120px|left]]<br />
different design for easy to build stages for the DIY microscope, z-stage, x-y servo controlled stage and more...<br />
<br />
|-<br />
| '''Project 26: [[How to start a nanolab]]'''<br />
[[File:nanolab.jpg|120px|left]]<br />
Because nanotechnology is situated at the interface of many disciplines it is best to organize some basic gear from various sources, such as a local electronics store, gardening shops or various online suppliers for household tools and electronic gadgets. You will need to set up some basic laboratory instruments for doing simple biology, chemistry, fabrication&manufacturing and some physical computing to connect everything to your computer.<br />
<br />
|-<br />
| '''Project 27: [[Magnetic stirrer]]'''<br />
[[File:NS_spinner_spinning.jpg|120px|left]]<br />
always useful to stir things automatically if you are lazy...<br />
<br />
|-<br />
|'''Project 28: [[DIY Micro Dispensing and Bio Printing]]'''<br />
[[File:nanofab.jpg|120px|left]]<br />
dispensing, cutting, printing, growing with a robotic platform. Aka NanoFabrik<br />
<br />
|-<br />
|'''Project 29: [[ArtScienceBangalore 2011-Synthetic Biology for Artists and Designers]]'''<br />
[[File:2011artsciblr.jpg|120px|left]]<br />
[http://2011.igem.org/Team:ArtScienceBangalore Link to IGEM site]<br />
<br />
Download the nicely illustrated booklet on "Dirt" here [[File:dirt_IGEM2011.pdf]]<br />
<br />
|-<br />
|'''Project 30: [[ArtScienceBangalore 2011-Nanotechnology for Artists and Designers]]'''<br />
[[File:Mindmap1.JPG|120px|left]]<br />
[http://openwetware.org/wiki/Biomod/2011/SRISHTI/ArtScienceBangalore Link to Biomod site]<br />
<br />
|-<br />
|'''Project 31: [[TEMPE(h) : THE INSTRUCTIONS]]'''<br />
[[File:tempe3.jpg|120px|left]]<br />
Tempe(h) is a fermented food. But it's not meant to preserve the material from rottenness. It's for style. Analogue meat they said. So, once you made your own tempe(h). Cook it! Eat it! <br />
<br />
<br />
<br />
|-<br />
|'''Project 32: [[Daphniaology]]'''<br />
[[File:daphnia_jar.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 33: [[Ants]]'''<br />
[[File:and_lenoir.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 34: [[Book: Homemade Biological Art]]'''<br />
[[File:homemade_BioArt.jpg|120px|left]]<br />
<br />
|-<br />
|'''Project 35: [[The Art of Hunting]]'''<br />
[[File:hunter.jpg|120px|left]]<br />
Baerman funnels, Berlese-Tullgren funnel, habitats<br />
<br />
|-<br />
|'''Project 36: [[Jugaad PCR Thermocyclers]]'''<br />
[[File:Hairdryer_PCR_lab2011.jpg|120px|left]]<br />
some links to alternative and DIY thermocycling experiments.<br />
<br />
|-<br />
|'''Project 37: [[MobileLabs]]'''<br />
[[File:mobilelabs.jpg|120px|left]]<br />
some links and ideas to mobile labs...<br />
<br />
|-<br />
|'''Project 38: [[Autonomous Public Lab]]'''<br />
[[File:Buildingabiodome.jpg|120px|left]]<br />
Blueprints for setting up a People's Research Lab<br />
<br />
|-<br />
|'''Project 39: [[Hackteria Merchandise and Logos]]'''<br />
[[File:Biohacker.png|120px|left]]<br />
loads of logos, t-shirt designs, stickers, flyers and posters<br />
<br />
|-<br />
|'''Project 40: [[Orbital Shaker]]'''<br />
[[File:orbital_shaker.jpg|120px|left]]<br />
shake it baby!<br />
<br />
|-<br />
|'''Project 41: [[Plant Electrophysiology]]'''<br />
[[File:web9.jpg|120px|left]]<br />
Yeah, even plants cant be protected from arduinos..<br />
<br />
|-<br />
|'''Project 42: [[Spin Coater]]'''<br />
[[File:spin_coater.jpg|120px|left]]<br />
DIY Spin Coater!<br />
<br />
|-<br />
|'''Project 43: [[NeuroNetworking]]'''<br />
[[File:dkbrain.jpg|120px|left]]<br />
Prototypes & design fiction into networking over brain data and biodata related to our brain function (fMRI, SNPs, EEG).<br />
How will we use brain data for social networking, neuromarketing, citizen science projects,etc.<br />
The main technical challenge is to create some open format for sharing brain data over the web (3D T1-weighted gradient-echo sequence (MP-RAGE)images in the nifti format http://nifti.nimh.nih.gov/nifti-1 , which is readable by FSL software (http://www.fmrib.ox.ac.uk/fsl/ into some 3D web browser data and image that would support crowdsourcing of brain data, pattern recognition and comparison of brain data).<br />
<br />
|-<br />
|'''Project 44: [[Garden 2012: Spherification of Hieronymus Bosch]]'''<br />
[[File:Spheres_big.jpg|120px|left]]<br />
Spherification experiments as a tribute to Hieronymus Bosch's Garden of Earthly Delights. In order to understand our new cosmology of minimal but also complex spheres (molecules, cells, vesicles, coacervates, microspheres), we decided to use spherification techniques and to try to design a version of Bosch's Garden of Earthly Delights like a catalogue of what is possible in terms of spheres today. Some of our spheres are eatable, other are perceived only by microscopes, some target taste buds other just molecules, we even tried to prepare a micro-meal for microorganisms entrapped in a type of mini-aquaria and systems. The three containers mimicking the original triptych are an early reflection of the importance of spheres for our idea of origin or the organic life but also present experiments in molecular gastronomy but also drug targeting systems and research in nano-bio technologies. Humans making spheres on all scales are like gods recreating some new garden with self-enclosed systems, ecologies and microspheres, which influence each other and interact.<br />
<br />
|-<br />
|'''Project 45: [[Bio+Hacker+Art-Space Tour California 2012]]'''<br />
[[File:cal_local.jpg|120px|left]]<br />
Picture protocoll of an intensive three week travel throug the venues of third culture in western California!<br />
<br />
<br />
|-<br />
|'''Project 46: [[Distillation]]'''<br />
[[File:distillation.jpg|120px|left]]<br />
Distillation at the Hackteria Distillery at Jagaa, Bangalore Dec. 2012 and other distillation rigs<br />
<br />
|-<br />
|'''Project 47: [[Do eat your laser Tofu (DEYLT)]]'''<br />
[[File:tof_icon.jpg|120px|left]]<br />
How to make your own tofu? From laser cutting a tofu mold to the preparation and the tasting.<br />
<br />
|-<br />
|'''Project 48: [[Randen-Burger or the art of documenting]]'''<br />
[[File:randen.jpg|120px|left]]<br />
...<br />
<br />
|-<br />
|'''Project 49: [[Hackteria_%26_SGMK_BioCyberKidzz#Yeast_powered_balloons|Yeast powered balloons a la BioCyberKidzz]]'''<br />
[[File:Maxiblasinator.jpg|120px|left]]<br />
This is a balloon, which inflates as a result of the yeast growing and interacting with its environment <br />
<br />
|-<br />
|'''Project 50: [[Hackteria_%26_SGMK_BioCyberKidzz#Bacterial_jewelery|Bacterial Jewelry a la BioCyberKidzz]]'''<br />
[[File:jewelry.jpg|120px|left]]<br />
The Bacterial Jewelry module comes as a take away amulet that has a living fingerprint of the participant. <br />
<br />
<br />
|-<br />
|'''Project 51: [[Hackteria_%26_SGMK_BioCyberKidzz#Body_Augmentation_and_Enhancement|Body Augmentation and Enhancement a la BioCyberKidzz]]'''<br />
[[File:elephantear.jpg|120px|left]]<br />
Talking about bioart and cyberbody modifications is accessible to everyone. <br />
<br />
|-<br />
|'''Project 52: [[Hackteria_%26_SGMK_BioCyberKidzz#LabBook|The LAB BOOK a la BioCyberKidzz]]'''<br />
[[File:laborbuch2.jpg|120px|left]]<br />
Recording observations is an important aspect of laboratory life. This module is to encourage the collection and recording of data. <br />
<br />
|-<br />
|'''Project 53: [[DIY Laser tweezer, cell trap, oligo synthesis]]'''<br />
[[File:tweez.png|120px|left]]<br />
Based on a paper of a laser tweezer made from an old DVD drive we go on...<br />
<br />
<br />
|-<br />
|'''Project 54: [[DIY Plasma generators]]'''<br />
[[File:plasma_siech.jpg|120px|left]]<br />
Where to get it, how to make it, the 4th state of matter.<br />
<br />
|-<br />
|'''Project 55: [[Hacked Hard-disc Centrifuge]]'''<br />
[[File:harddisc_centrifuge.jpg|120px|left]]<br />
Get an old harddisc, laser cut or 3D pring a eppi-holder, run it...<br />
<br />
|-<br />
|'''Project 56: [[Agar is the Media]]'''<br />
[[File:agar_media.jpg|120px|left]]<br />
The key technologies of every genetic and molecular biology lab – the gel electrophoresis chamber...<br />
<br />
<br />
|-<br />
|'''Project 57: [[BubbleBots]]'''<br />
[[File:BubbleBots.jpg|120px|left]]<br />
Tiny little tubular rockets generating bubbles to propel themselves through water.<br />
<br />
|-<br />
|'''Project 58: [[Ganzfeld experiment]]'''<br />
[[File:Ganzfeld experiment.JPG|120px|left]]<br />
This is a modified experiment from the 70s and which can be used as a great icebreaker activity for building trust.<br />
<br />
|-<br />
|'''Project 59: [[Rubber_Hand_Illusion_Experiment_variations]]'''<br />
[[File:Wooden-hand.jpeg|120px|left]]<br />
a variation developed through trying this workshop on a large number of people, and not having funding to get loads of rubber hands. So we made the hands in the workshop.<br />
<br />
|-<br />
|'''Project 60: [[Internet of Bugs'n'Beasts]]'''<br />
[[File:bugsnbug.jpeg|120px|left]]<br />
starting soon<br />
<br />
|-<br />
|'''Project 61: [[KIMCHI or the Art of Fermentation]]'''<br />
[[File:kimchi01.jpg|120px|left]]<br />
HomeMade KIMCHI from scratch.....<br />
The final taste all depends on the amount of salt, dehydration, fermentation and temperature monitor.<br />
<br />
<br />
<br />
|-<br />
|'''Project 62: [[Science For Poets]]'''<br />
[[File:Webbana.jpg|120px|left]]<br />
LifeSciences Workshop for Artists & Designers in Bangalore 2013, collaborators of biodesign.cc<br />
<br />
|-<br />
|'''Project 63: [[Zebrafish Hacking: The Plant Animal]]'''<br />
[[File:ZEBRAFISH.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 64: [[Wild OpenPCR]]'''<br />
[[File:WildOpenPCR.JPG|120px|left]]<br />
Building an OpenPCR without the kit, just from commercially available parts. With additional information on how to do it.<br />
<br />
<br />
|-<br />
|'''Project 65: [[Explorations in BioLuminescence]]'''<br />
[[File:bioluminescence.jpg|120px|left]]<br />
From fireflies to stinky squids... the future is bright<br />
<br />
<br />
|-<br />
|'''Project 66: [[Say Cheese]]'''<br />
[[File:Cheesemaking.jpg|120px|left]]<br />
make your own fresh cheese<br />
<br />
<br />
|-<br />
|'''Project 67: [[Hack a Taq]]'''<br />
[[File:Hack-a-taq.jpg|120px|left]]<br />
Testing out the Wild OpenPCR and purifying your own Taq from Open Biotechnology - using primers to detect coliform bacteria <br />
<br />
|-<br />
|'''Project 68: [[Poor Man's BioPrinter]]'''<br />
[[File:poorBioPrinter.jpg|120px|left]]<br />
There are many ways of bioprinting...<br />
<br />
|-<br />
|'''Project 69: [[DIYbio Prototypes Collection & Hackteria archive]]'''<br />
[[Image:2.jpg|120px|left]]<br />
Collection of open biology and DIYbio prototypes created by members of the Hackteria network, such as DNA Lab-in-Box, Gene gun etc. This archive of exhibition artefacts supports open science and citizen science advocacy and showcases the open hardware possibilities of supporting research in the Global South.<br />
<br />
|-<br />
|'''Project 70: [[Urban Cuisine]]'''<br />
[[Image:snail_huntin.jpg|120px|left]]<br />
Various explorations into urban ecologies and recipes to hunt, collect, grow, cook, eat your findings <br />
<br />
|-<br />
|'''Project 71: [[Actinomycetes Tournament: Open antibiotics discovery]]'''<br />
[[Image:acti.jpg|120px|left]]<br />
In this project we want to screen soil bacteria, such as Actinomyces, Streptomyces and Micromonospora for antibiotic properties. It is a global citizen science initiative started by DIYbio Waag, which uses game elements (collecting and tournaments) to support open drug discovery of antibiotics. We will isolate actinomycetes from soil samples around the world and screen with Micrococcus luteus or similar microbe to determine their antibiotic properties. Images of inoculated plates will be shared online and based on their strengths (zones of growth inhibition) they will become part of a collection or initiate tournaments between the most successful examples. We will sequence the most powerful ones. We expect cooperation with various DIYbio organizations, microbiology departments and science enthusiasts. <br />
Picture: Antibiotic Producing Actinomycetes By adonofrio (Biology101.org)<br />
<br />
|-<br />
|'''Project 72: [[High Voltage Epigenetics]]'''<br />
[[Image:high_voltage_yeast.jpg|120px|left]]<br />
Is it real or is it epigenetics? A novel method is described, which, on the basis of the short-term application of electrostatic fields, results in lasting beneficial and desirable properties in animals and plants. The culture of fish, particularly of edible fish but also of ornamental fish, is genuinely revolutionised. <br />
<br />
|-<br />
|'''Project 73: [[How to make a proper Barber Trap]]'''<br />
[[Image:BarberTrap.png|120px|left]]<br />
<br />
|-<br />
|'''Project 74: [[doms Mobile vj lab]]'''<br />
As time past I have grow huge need for mobile vj lab for all indoor and outdoor visual activity.<br />
From my experience till now it has to be paced with lots of power, projector, cams, microscope,sound system, bio lab tools (pipettes, slide glass...),many different vj tools(blinking led...), lasers, traps and containers for live organisms. <br />
Probably some other stuff that I will find useful like knife, glue, bugs 2d aquarium, different plugs for charging and powering<br />
that you always find helpful on terrain.<br />
Of course all together should not be to heavy (5kg max but rather 3kg)....<br />
... <br />
<br />
|-<br />
|'''Project 75: [[openTrap]]'''<br />
[[Image:FoxTrap.jpg|120px|left]]<br />
More and more we are looking into various traps for catching larger animals such as rats, foxes, pigeons and more. Re-developing some popular designs, adapting them, make them open source for digital fabrication<br />
... <br />
<br />
<br />
|-<br />
|'''Project 76: [[DIY_NanoDrop]]'''<br />
[[Image:DIYNanoDrop.jpg|120px|left]]<br />
Microvolume Spectrophotometers and Fluorospectrometers are innovative products used to quantitate micro-volumes of sample. You want one? Build your own.<br />
... <br />
<br />
<br />
|-<br />
|'''Project 77: [[Nail Studio Micro Fluidics]]'''<br />
[[Image:NailStudioMicroFluidics.jpg|120px|left]]<br />
Nail studios are the girls secret hight tech labs with UV light curing gel and other fancy stuff. Let's hack it to do low cost microfluidics.<br />
... <br />
<br />
|-<br />
|'''Project 78: [[Mobile Power Solutions]]'''<br />
[[Image:PowerSolutions.jpg|120px|left]]<br />
You need mobile electrical power - On how to build Graphene Supercapacitors, Peltier electricity from fire and more...<br />
<br />
|-<br />
|'''Project 79: [[Fish-Hacking]]'''<br />
[[Image:IMG_2593.JPG|120px|left]]<br />
Ever tried to listen into the electrocommunication behavior of weakly electric fish? Recent experiments and general info about black ghost knifefish, inspired by [http://hackteria.org/wiki/index.php/Fish-to-Brain_Interfaces,_Antony_Hall,_Ljubljana Tony Hall's work on ENKI technology]. They have also been shown to be useful for biomonitoring of pullutants in water! Fish-2-pd interfaces are already working nicely.<br />
<br />
|-<br />
|'''Project 80: [[Long Range Microphone for Cicadas & Cricket Hunting]]'''<br />
[[Image:Garengpung.jpg|120px|left]]<br />
Imagine you are in the forest, rivers, or mountains and some noises just appears and hunting your ears and you kind of wanted to be sure what is it and where is it come from. This long range microphone might help you to solve the mistery. But, as the title says it will be used for hunting cricket and cicadas a.k.a garengpung, then we must have some ideas about how the cricket and cicadas a.k.a garengpung sounds like. - still under construction. Hhaha... so is everything else on that wiki :-)<br />
<br />
|-<br />
|'''Project 81: [[DIY Microbial Fuel Cells]]'''<br />
[[Image:Mud_Battery.png|120px|left]]<br />
Imagine you are in the forest and create a bit of electricity from mud...<br />
<br />
|-<br />
|'''Project 82: [[Water Sampling Coconut]]'''<br />
[[Image:CoconutSensor.jpg|120px|left]]<br />
GPS and water sensors in a coconut go explore the rivers.<br />
<br />
|-<br />
|'''Project 83: [[Psychokinetic Action of Young Chicks]]'''<br />
[[Image:PsychoCkicks.jpg|120px|left]]<br />
What if there was such a thing as Psychokinetics - let's test. <br />
<br />
|-<br />
|'''Project 84: [[Aquatic Malaria Extinction Robot - TEAM1]]'''<br />
[[Image:Malaria_siech_dusjagr.jpg|120px|left]]<br />
Some thoughts and experiments about malaria reduction, mosquito killing and stinky socks<br />
<br />
|-<br />
|'''Project 85: [[SATW - Do-it-yourself von Laborgeräten in der Bioanalytik]]'''<br />
[[Image:SATW-DIY_kit.jpg|120px|left]]<br />
Co-Development of a workshop on DIY laboratory instruments for bioanalytics, colorimetry, low-cost microcontrollers and simple DIY electronics, enzymatic assays. With a concept of "teach the teachers", we are looking into DIY lab-tools as means of a pedagogic tool for interdisciplinary thinking at the biomedical/engineering/molecular interface. Coproduced with SATW, Swiss Academy of Technical Sciences, Hackteria and FHNW, School for Lifesciences.<br />
<br />
|-<br />
|'''Project 86: [[GynePUNK biolabs]]'''<br />
[[Image:smell_my_finger.jpg|120px|left]]<br />
key words: ANARCHA biopunk - DIY bodyaction - radical SelFpower - Analisis of fluids - self citology - DIY equipment<br />
<br />
BIO-reSEARCH is part of [[Pechblenda]] tentacles, mixed with [http://anarchagland.hotglue.me AnarchaGland] researchs and [http://gynepunk.tumblr.com/ GynePUNK biolabs]<br />
<br />
|-<br />
|'''Project 87: [[Ayam Cemani - ... or the Black Chicken Project]]'''<br />
[[Image:Ayam cemani kid.jpg|120px|left]]<br />
Ayam means "chicken" in Indonesian and Cemani is "completely black" in Javanese.<br />
<br />
|-<br />
|'''Project 88: [[Lick the Moonmilk]]'''<br />
[[Image:Mondmlichloch.png|120px|left]]<br />
Exploring the origin and use of moonmilk. Cave safari to the Moondmilchloch in Lucern.<br />
<br />
|-<br />
|'''Project 89: [[Elektrowetting]]'''<br />
[[Image:Elektrowetting.png|120px|left]]<br />
Playing with electro-wetting effect. Trying to finally make that desktop DNA synthesizer.<br />
<br />
|-<br />
|'''Project 90: [[Nucleic_Acid_Quantitation]]'''<br />
[[Image:nucleicAcidAbsorbtion.png|120px|left]]<br />
Nucleic_Acid_Quantitation<br />
<br />
|-<br />
|'''Project 91: [[Wild openQCM]]'''<br />
[[Image:wild_openQCM.jpg|120px|left]]<br />
Make your free open wild openQCM aka artificial nose<br />
<br />
|-<br />
|'''Project 92: [[CocoMake7]]'''<br />
[[Image:CocoMake7_boardShape.png|120px|left]]<br />
A jugaad ultra low-cost clone to the Makey Makey, but based on QTouchADC sensing, local manufacturing and coconuts<br />
<br />
|-<br />
|'''Project 93: [[Nano hacking science and DIY/DIWO low cost diagnosis]]'''<br />
[[Image:IMG_0845.png|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 94: [[MicroMacroPleasure]]'''<br />
[[Image:Ursspeculum.jpg|120px|left]]<br />
Dildomancy hacking science and DIY/DIWO low cost pleasure and vaginal trainning<br />
<br />
|-<br />
|'''Project 95: [[Biocomons.Lab]]'''<br />
[[Image:11738011_692237857544097_3209041620878190282_n.jpg|120px|left]]<br />
A meeting to define the bio-value and the bio-capital in the era of biotechnology. 6 days of unconferences and workshops to push further the [http://www.bio-commons.org/ Bio-Commons research project].<br />
<br />
|-<br />
|'''Project 96: [[Mobile Plant Analysis Lab @ pifCamp, Slovenia]]'''<br />
[[Image:DusjagrLab_pifCamp.jpg|120px|left]]<br />
PIFcamp - Summer Hacking Camp, 3 – 9 August 2015 @ Trenta, Slovenia<br />
<br />
|-<br />
|'''Project 97: [[Jugaad PhoneScope]]'''<br />
[[Image:Lens_closeUp.jpg|120px|left]]<br />
Very simple and self-made DIY lens to attach to your smart-phone and turning it into a nice microscope.<br />
<br />
|-<br />
|'''Project 98: [[openlab gynecologyDIT]]'''<br />
[[Image:Dr-harry-waites-vibrator.jpg|120px|left]]<br />
Residence Fablab PlateformeC [www.plateforme-c.org] One of the topics or lines to explore Antropocene Or not ! PLASTICOCENE<br />
<br />
|-<br />
|'''Project 99: [[Euglena Burger]]'''<br />
[[Image:japan_euglenaBurger.png|120px|left]]<br />
The Euglena Burger - half animal and half plant. finally working on the recipes, upscaling using custom made home bio-reactors and looking forward to serve it to our friends.<br />
<br />
|-<br />
|'''Project 100: [[Save the Whales]]'''<br />
[[Image:pandas_will_save_the_whales.jpg|120px|left]]<br />
No more words - We need ACTION!<br />
<br />
|-<br />
|'''Project 101: [[PCR your DNA Workshop]]'''<br />
[[Image:WildOpenPCR.jpg|120px|left]]<br />
All you need to prepare for a basic workshop on getting some DNA, setting up a PCR, and running the gel to see some results in a day-long workshop<br />
<br />
|-<br />
|'''Project 102: [[Worm Composting]]'''<br />
[[Image:Wormcompost_3months.jpeg|120px|left]]<br />
Indoors or outdoors, a great way to reduce your garbage amounts and get super-rich compost for the garden<br />
<br />
|-<br />
|'''Project 103: [[Open Source Estrogen]]'''<br />
[[File:PIIS001078241100179X.gr1.lrg.jpg|120px|left]]<br />
Community page for DIY estrogen!!!<br />
<br />
|-<br />
|'''Project 104: [[Feuerphilosophen]]'''<br />
[[File:Alchemy.jpg|120px|left]]<br />
Alchemy Reenactment Session<br />
<br />
<br />
|-<br />
|'''Project 105: [[Fostering Duckweed]]'''<br />
[[File:DSC01035.jpg|120px|left]]<br />
Duckweed might be the food of the future? Can we grow it from our own pee as fertilizer?<br />
<br />
<br />
|-<br />
|'''Project 106: [[Völlig Übertriibe]]'''<br />
[[File:Mark_aquarium.jpg|120px|left]]<br />
Session in Schaffhausen for exploring BioVJing and other crazy Hydra-hacks<br />
<br />
<br />
|-<br />
|'''Project 107: [[Laser-Technologie 2000]]'''<br />
[[File:LaserTechnologie_2000_circuit.png|120px|left]]<br />
simple constant current circuit for hacking lasers<br />
<br />
<br />
|-<br />
|'''Project 109: [[Chrisper-Chäsli]]'''<br />
[[File:DIY_CRISPR_Kits.jpg|120px|left]]<br />
Some reflections on gene-editing and making cheese<br />
<br />
<br />
|-<br />
|'''Project 110: [[PRG Ikan Hobi - GMO fish geeking]]'''<br />
[[File:PRK_ikan_Jan2017_lifepatch.jpg|120px|left]]<br />
Geeking around with zebra-fish, CRISPR and the GM-fish (formerly know as glow-fish) we found on the market in Yogyakarta. Seems the Indonesian regulations are supporting the use of GM fish for sports, hobby, crafts and ornamental use!<br />
<br />
<br />
|-<br />
|'''Project 111: [[DIY chemistry analysis of food]]'''<br />
[[File:CuvettesGrapesmall.jpg|120px|left]]<br />
Series of protocols in development for extracting and analysing micronutrients in foods, using DIY methods and without the need for lab access.<br />
<br />
<br />
|-<br />
|'''Project 112: [[Make you own CRISPR-Kit]]'''<br />
[[File:TheCRISPR.jpg|120px|left]]<br />
Creative workshop at Schloss Solitude. Design your own CRISPR-Kit.<br />
<br />
|-<br />
|'''Project 112: [[Miso]]'''<br />
[[File:Dplus_miso_mixSojaRice.jpg|120px|left]]<br />
Miso (味噌 or みそ) making Workshop at Dimension Plus TW<br />
<br />
|-<br />
|'''Project 113: [[DIY Single Photon Generator]]'''<br />
[[File:SinglePhoton.jpg|120px|left]]<br />
Finding a hack to generate single photons. Quantum-Nano-Dots, Cristals, Ions?<br />
<br />
|-<br />
|'''Project 114: [[Gär Lämpli]]'''<br />
[[File:GarLampli_box_Pink.jpg|120px|left]]<br />
Check the Temperature and Time profile of your ferments... with just 1 LED<br />
<br />
|-<br />
|'''Project 115: [[Back to Bombyx]]'''<br />
[[File:LiftOfSpiderPrint.JPG|120px|left]]<br />
Exploring silk, again.<br />
<br />
|-<br />
|'''Project 116: [[Circular Chromatography]]'''<br />
[[File:Circular_chromatography.jpg|120px|left]]<br />
Started at [http://wiki.artscienceblr.org/wiki/index.php/Circular_chromatography (Art)ScienceBLR's site], enjoy concentric separation of matter - from soil, and developing sugars<br />
<br />
|-<br />
|'''Project 117: [[DIY Mutagenesis]]'''<br />
[[File:Mutagenesis.png|120px|left]]<br />
à la ur-institute<br />
<br />
<br />
|-<br />
|'''Project 118: [[Trans Organs on a Chip]]'''<br />
[[File:Transorgans_v1.png|120px|left]]<br />
grow your own transorgans for fully (micro) fluid(ic) exchange!<br />
<br />
|-<br />
|'''Project 119: [[Participation in Decision Making by Members of DIY Communites]]'''<br />
[[File:39890639411_d41caf1935_k.jpg|120px|left]]<br />
Research project on the needs of the community for sucessful collaboration with institutions<br />
<br />
<br />
|-<br />
|'''Project 120: [[HiSeq2000_-_Next_Level_Hacking]]'''<br />
[[File:HiSeq.jpg|120px|left]]<br />
Let's hack that HiSeq Next Level Sequencing machine and make it open!<br />
<br />
|-<br />
|'''Project 121: [[MakeBlock Me High-Power Encoder Motor Driver]]'''<br />
[[File:12045_01-500x500.JPG|120px|left]]<br />
Open Leaking Stuff<br />
<br />
|-<br />
|'''Project 122: [[Slime Mould]]'''<br />
[[File:Slime_mould_in_situ.JPG|120px|left]]<br />
more than the traveling salesman's problem<br />
<br />
<br />
<!-- Projects end --></div>Derishushttp://www.hackteria.org/wiki/index.php?title=Collection_of_DIY_Biology,_Open_Source_Art_Projects&diff=28391Collection of DIY Biology, Open Source Art Projects2018-06-07T15:32:44Z<p>Derishus: </p>
<hr />
<div><!-- Projects --><br />
<div class="alertBox1"><br />
{| class="alert"<br />
!Projects<br />
<br />
|-<br />
|'''Project 0: [[WetWare]]'''<br />
[[File:WetWare_s.jpg|120px|left]]<br />
Where to shop for materials<br />
<br />
|-<br />
|'''Project 1: [[The Bacterial Net- Microbial Telecommunications.]]'''<br />
[[Image:2.jpg|120px|left]]<br />
Quorum sensing is a type of decision-making process used by decentralized groups to coordinate behavior. Many species of bacteria use quorum sensing to coordinate their gene expression according to the local density of their population.The Bacterial Net attempts to transfer this decision making process over the Internet. Using Microcontollers and sensors to detect bacterial population and a public web service http://www.pachube.com that helps transfer sensor information. The Bacterial Net is an exploration in bringing the Internet into the Natural Ecosystem. <br />
<br />
|-<br />
|'''Project 2: [[Bioelectronix]]'''<br />
<br />
[[Image:bioelectronics_after_casting.jpg|120px|left]]<br />
<br />
Integrating electronics into biological culture devices needs different approaches. the use of silicone-rubbers for sealing off the wet/humid bio-part from the silicon circuits and sensors allows the making of hybrid bioelectronic devices. <br />
|-<br />
| '''Project 3: [[Synthetic Biology for Artists and Designers: A primer]]'''<br />
[[File:synbio_cover.jpg|120px|left]]<br />
An Illustrated [http://hackteria.org/wiki/images/a/a1/Handbook.pdf <strong>handbook</strong>] that shows you how to work with MIT's parts registry<br />
|-<br />
| '''Project 4: [[ArtScienceBangalore 2009-Synthetic Biology for Artists and Designers]]'''<br />
[[File:Whiteboard.jpg|120px|left]]<br />
<br />
see also the [http://prix2011.aec.at/winner/3128/ docu for Prix Ars Electronica]<br />
<br />
<br />
|-<br />
|'''Project 5: [[DIY microscopy]]'''<br />
[[File:cam_ani_sm.gif|left]]<br />
<br />
a small hack to the optics of a standard webcam with an adjustable focus-lens, allows to create video data, with a magnification of around 100 to 400x (depending on the model) at a working distance of a few mm. The highest magnifications can be achieved by inverting (putting upside down) the lens. Addition of good lighting by the use of leds allows to create images using a bright-field method (shine through the stuff) or dark field method (look at the reflections and scattering). <br />
<br />
|-<br />
|'''Project 6:[[DIY $25 Gel Box]]'''<br />
[[File:DSC 8050.jpg|120px|left]]<br />
Building your own Electrophoresis Gel Box is easy. Here's how you do it!<br />
<br />
|-<br />
| '''Project 7: [[bio habitats]]'''<br />
[[File:biohabitat.jpg|120px|left]]<br />
instead of using standard lab culture tools, such as flasks or petri-dishes, artists might want to design their own devices to culture and inhabitate living organisms and keep them alive. simple devices can be cast in silicone to incorporate all the necessary environemtal conditions for long time survival of microorganisms.<br />
<br />
|-<br />
| '''Project 8: [[DIY Microfluidics]]'''<br />
[[File:Microfluidicwikiimage.png|120px|left]]<br />
<br />
|-<br />
| '''Project 9: [[DIY Plant Tissue culture and Engineering]]'''<br />
[[File:plant_culture.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 10: [[Pd_microscope]]'''<br />
[[File:pd_microscope.png|120px|left]]<br />
Pd_microscope is the software package that you can use to control your DIY microscope. It's a work in progress<br />
<br />
|-<br />
| '''Project 11: [[DIY spectroscopy]]'''<br />
[[File:spektral_tag1.jpg|120px|left]]<br />
Spectroscopy means the analysis of natural phenomena according to its energy distribution. In case of the electromagnetic spectra, ranging from x-ray, visible light, infrated to radio frequencies, this can be used to analyse chemical compounds, follow biochemical reactions or get information of the composition of stars. This project tries utilize webcams, CD/DVD and some construction material, to get a spectral analysis of ultra-violett and visible light sources, which might be used to follow enzymatic and metabolic reactions in cultured microorganisms and bacteria.<br />
<br />
|-<br />
| '''Project 12: [[ArtScienceBangalore 2010-Synthetic Biology for Artists and Designers]]'''<br />
[[File:Asb_2010_group.jpg|120px|left]]<br />
A bunch of regular people with very little idea of Synthetic Biology but ready to pick up eveything about it.<br />
<br />
Download the nicely illustrated Comic Book on Synthetic Ecologies here [[File:IGEM_Comic_2010.pdf]]<br />
<br />
|-<br />
| '''Project 13: [[DIY handheld centrifuge]]'''<br />
[[File:HandHeldCent.jpg |120px|left]]<br />
<br />
|-<br />
| '''Project 14: [[DIY table top centrifuge]]'''<br />
[[File:Table_top.JPG|120px|left]]<br />
<br />
|-<br />
| '''Project 15: [[DIY Incubator]]'''<br />
[[File:DSC03096.JPG|120px|left]]<br />
<br />
<br />
|-<br />
| '''Project 16: [[DIY Fermentation or the Art of making Wine]]'''<br />
[[File:diy_fermentation.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 17: [[Hacked Optical Mouse]]'''<br />
[[File:optical_mouse_hack.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 18: [[DIY Sterlisation Hood]]'''<br />
[[File:DSC03946.JPG |120px|left]]<br />
<br />
|-<br />
| '''Project 19: [[DIY Water Bath]]'''<br />
[[File:DSC04040.JPG|120px|left]]<br />
<br />
|-<br />
| '''Project 20: [[GFP Protocol Jugaad]]'''<br />
[[File:MG_7283.JPG|120px|left]]<br />
Home made GFP bacteria<br />
<br />
|-<br />
| '''Project 21: [[ReacTable]]'''<br />
[[File:DSC04180.JPG|120px|left]]<br />
Gives information and protocals of equipment and bacteria put on it<br />
<br />
|-<br />
| '''Project 22: [[Algae Culture at Home]]'''<br />
[[File:Plankton.jpg|120px|left]]<br />
Grow your own algae at home with some plastic bottles, lighting and air bubbles<br />
<br />
|-<br />
| '''Project 23: [[Mobile Phone Field Microscope]]'''<br />
[[File:hacked_phone.jpg|120px|left]]<br />
Modify a mobile phone into a useful field microscope by replacing the lens with a cheap flipped wemcam lens..<br />
<br />
|-<br />
| '''Project 24: [[DIY turbidity meters]]'''<br />
[[File:turbidity_shield.jpg|120px|left]]<br />
simple light absorption measurements for water monitoring or nanoparticle detection using an arduino, a laser or LED and an LDR.<br />
<br />
|-<br />
| '''Project 25: [[Laser-cut microscopy stages]]'''<br />
[[File:miniScope.jpg|120px|left]]<br />
different design for easy to build stages for the DIY microscope, z-stage, x-y servo controlled stage and more...<br />
<br />
|-<br />
| '''Project 26: [[How to start a nanolab]]'''<br />
[[File:nanolab.jpg|120px|left]]<br />
Because nanotechnology is situated at the interface of many disciplines it is best to organize some basic gear from various sources, such as a local electronics store, gardening shops or various online suppliers for household tools and electronic gadgets. You will need to set up some basic laboratory instruments for doing simple biology, chemistry, fabrication&manufacturing and some physical computing to connect everything to your computer.<br />
<br />
|-<br />
| '''Project 27: [[Magnetic stirrer]]'''<br />
[[File:NS_spinner_spinning.jpg|120px|left]]<br />
always useful to stir things automatically if you are lazy...<br />
<br />
|-<br />
|'''Project 28: [[DIY Micro Dispensing and Bio Printing]]'''<br />
[[File:nanofab.jpg|120px|left]]<br />
dispensing, cutting, printing, growing with a robotic platform. Aka NanoFabrik<br />
<br />
|-<br />
|'''Project 29: [[ArtScienceBangalore 2011-Synthetic Biology for Artists and Designers]]'''<br />
[[File:2011artsciblr.jpg|120px|left]]<br />
[http://2011.igem.org/Team:ArtScienceBangalore Link to IGEM site]<br />
<br />
Download the nicely illustrated booklet on "Dirt" here [[File:dirt_IGEM2011.pdf]]<br />
<br />
|-<br />
|'''Project 30: [[ArtScienceBangalore 2011-Nanotechnology for Artists and Designers]]'''<br />
[[File:Mindmap1.JPG|120px|left]]<br />
[http://openwetware.org/wiki/Biomod/2011/SRISHTI/ArtScienceBangalore Link to Biomod site]<br />
<br />
|-<br />
|'''Project 31: [[TEMPE(h) : THE INSTRUCTIONS]]'''<br />
[[File:tempe3.jpg|120px|left]]<br />
Tempe(h) is a fermented food. But it's not meant to preserve the material from rottenness. It's for style. Analogue meat they said. So, once you made your own tempe(h). Cook it! Eat it! <br />
<br />
<br />
<br />
|-<br />
|'''Project 32: [[Daphniaology]]'''<br />
[[File:daphnia_jar.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 33: [[Ants]]'''<br />
[[File:and_lenoir.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 34: [[Book: Homemade Biological Art]]'''<br />
[[File:homemade_BioArt.jpg|120px|left]]<br />
<br />
|-<br />
|'''Project 35: [[The Art of Hunting]]'''<br />
[[File:hunter.jpg|120px|left]]<br />
Baerman funnels, Berlese-Tullgren funnel, habitats<br />
<br />
|-<br />
|'''Project 36: [[Jugaad PCR Thermocyclers]]'''<br />
[[File:Hairdryer_PCR_lab2011.jpg|120px|left]]<br />
some links to alternative and DIY thermocycling experiments.<br />
<br />
|-<br />
|'''Project 37: [[MobileLabs]]'''<br />
[[File:mobilelabs.jpg|120px|left]]<br />
some links and ideas to mobile labs...<br />
<br />
|-<br />
|'''Project 38: [[Autonomous Public Lab]]'''<br />
[[File:Buildingabiodome.jpg|120px|left]]<br />
Blueprints for setting up a People's Research Lab<br />
<br />
|-<br />
|'''Project 39: [[Hackteria Merchandise and Logos]]'''<br />
[[File:Biohacker.png|120px|left]]<br />
loads of logos, t-shirt designs, stickers, flyers and posters<br />
<br />
|-<br />
|'''Project 40: [[Orbital Shaker]]'''<br />
[[File:orbital_shaker.jpg|120px|left]]<br />
shake it baby!<br />
<br />
|-<br />
|'''Project 41: [[Plant Electrophysiology]]'''<br />
[[File:web9.jpg|120px|left]]<br />
Yeah, even plants cant be protected from arduinos..<br />
<br />
|-<br />
|'''Project 42: [[Spin Coater]]'''<br />
[[File:spin_coater.jpg|120px|left]]<br />
DIY Spin Coater!<br />
<br />
|-<br />
|'''Project 43: [[NeuroNetworking]]'''<br />
[[File:dkbrain.jpg|120px|left]]<br />
Prototypes & design fiction into networking over brain data and biodata related to our brain function (fMRI, SNPs, EEG).<br />
How will we use brain data for social networking, neuromarketing, citizen science projects,etc.<br />
The main technical challenge is to create some open format for sharing brain data over the web (3D T1-weighted gradient-echo sequence (MP-RAGE)images in the nifti format http://nifti.nimh.nih.gov/nifti-1 , which is readable by FSL software (http://www.fmrib.ox.ac.uk/fsl/ into some 3D web browser data and image that would support crowdsourcing of brain data, pattern recognition and comparison of brain data).<br />
<br />
|-<br />
|'''Project 44: [[Garden 2012: Spherification of Hieronymus Bosch]]'''<br />
[[File:Spheres_big.jpg|120px|left]]<br />
Spherification experiments as a tribute to Hieronymus Bosch's Garden of Earthly Delights. In order to understand our new cosmology of minimal but also complex spheres (molecules, cells, vesicles, coacervates, microspheres), we decided to use spherification techniques and to try to design a version of Bosch's Garden of Earthly Delights like a catalogue of what is possible in terms of spheres today. Some of our spheres are eatable, other are perceived only by microscopes, some target taste buds other just molecules, we even tried to prepare a micro-meal for microorganisms entrapped in a type of mini-aquaria and systems. The three containers mimicking the original triptych are an early reflection of the importance of spheres for our idea of origin or the organic life but also present experiments in molecular gastronomy but also drug targeting systems and research in nano-bio technologies. Humans making spheres on all scales are like gods recreating some new garden with self-enclosed systems, ecologies and microspheres, which influence each other and interact.<br />
<br />
|-<br />
|'''Project 45: [[Bio+Hacker+Art-Space Tour California 2012]]'''<br />
[[File:cal_local.jpg|120px|left]]<br />
Picture protocoll of an intensive three week travel throug the venues of third culture in western California!<br />
<br />
<br />
|-<br />
|'''Project 46: [[Distillation]]'''<br />
[[File:distillation.jpg|120px|left]]<br />
Distillation at the Hackteria Distillery at Jagaa, Bangalore Dec. 2012 and other distillation rigs<br />
<br />
|-<br />
|'''Project 47: [[Do eat your laser Tofu (DEYLT)]]'''<br />
[[File:tof_icon.jpg|120px|left]]<br />
How to make your own tofu? From laser cutting a tofu mold to the preparation and the tasting.<br />
<br />
|-<br />
|'''Project 48: [[Randen-Burger or the art of documenting]]'''<br />
[[File:randen.jpg|120px|left]]<br />
...<br />
<br />
|-<br />
|'''Project 49: [[Hackteria_%26_SGMK_BioCyberKidzz#Yeast_powered_balloons|Yeast powered balloons a la BioCyberKidzz]]'''<br />
[[File:Maxiblasinator.jpg|120px|left]]<br />
This is a balloon, which inflates as a result of the yeast growing and interacting with its environment <br />
<br />
|-<br />
|'''Project 50: [[Hackteria_%26_SGMK_BioCyberKidzz#Bacterial_jewelery|Bacterial Jewelry a la BioCyberKidzz]]'''<br />
[[File:jewelry.jpg|120px|left]]<br />
The Bacterial Jewelry module comes as a take away amulet that has a living fingerprint of the participant. <br />
<br />
<br />
|-<br />
|'''Project 51: [[Hackteria_%26_SGMK_BioCyberKidzz#Body_Augmentation_and_Enhancement|Body Augmentation and Enhancement a la BioCyberKidzz]]'''<br />
[[File:elephantear.jpg|120px|left]]<br />
Talking about bioart and cyberbody modifications is accessible to everyone. <br />
<br />
|-<br />
|'''Project 52: [[Hackteria_%26_SGMK_BioCyberKidzz#LabBook|The LAB BOOK a la BioCyberKidzz]]'''<br />
[[File:laborbuch2.jpg|120px|left]]<br />
Recording observations is an important aspect of laboratory life. This module is to encourage the collection and recording of data. <br />
<br />
|-<br />
|'''Project 53: [[DIY Laser tweezer, cell trap, oligo synthesis]]'''<br />
[[File:tweez.png|120px|left]]<br />
Based on a paper of a laser tweezer made from an old DVD drive we go on...<br />
<br />
<br />
|-<br />
|'''Project 54: [[DIY Plasma generators]]'''<br />
[[File:plasma_siech.jpg|120px|left]]<br />
Where to get it, how to make it, the 4th state of matter.<br />
<br />
|-<br />
|'''Project 55: [[Hacked Hard-disc Centrifuge]]'''<br />
[[File:harddisc_centrifuge.jpg|120px|left]]<br />
Get an old harddisc, laser cut or 3D pring a eppi-holder, run it...<br />
<br />
|-<br />
|'''Project 56: [[Agar is the Media]]'''<br />
[[File:agar_media.jpg|120px|left]]<br />
The key technologies of every genetic and molecular biology lab – the gel electrophoresis chamber...<br />
<br />
<br />
|-<br />
|'''Project 57: [[BubbleBots]]'''<br />
[[File:BubbleBots.jpg|120px|left]]<br />
Tiny little tubular rockets generating bubbles to propel themselves through water.<br />
<br />
|-<br />
|'''Project 58: [[Ganzfeld experiment]]'''<br />
[[File:Ganzfeld experiment.JPG|120px|left]]<br />
This is a modified experiment from the 70s and which can be used as a great icebreaker activity for building trust.<br />
<br />
|-<br />
|'''Project 59: [[Rubber_Hand_Illusion_Experiment_variations]]'''<br />
[[File:Wooden-hand.jpeg|120px|left]]<br />
a variation developed through trying this workshop on a large number of people, and not having funding to get loads of rubber hands. So we made the hands in the workshop.<br />
<br />
|-<br />
|'''Project 60: [[Internet of Bugs'n'Beasts]]'''<br />
[[File:bugsnbug.jpeg|120px|left]]<br />
starting soon<br />
<br />
|-<br />
|'''Project 61: [[KIMCHI or the Art of Fermentation]]'''<br />
[[File:kimchi01.jpg|120px|left]]<br />
HomeMade KIMCHI from scratch.....<br />
The final taste all depends on the amount of salt, dehydration, fermentation and temperature monitor.<br />
<br />
<br />
<br />
|-<br />
|'''Project 62: [[Science For Poets]]'''<br />
[[File:Webbana.jpg|120px|left]]<br />
LifeSciences Workshop for Artists & Designers in Bangalore 2013, collaborators of biodesign.cc<br />
<br />
|-<br />
|'''Project 63: [[Zebrafish Hacking: The Plant Animal]]'''<br />
[[File:ZEBRAFISH.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 64: [[Wild OpenPCR]]'''<br />
[[File:WildOpenPCR.JPG|120px|left]]<br />
Building an OpenPCR without the kit, just from commercially available parts. With additional information on how to do it.<br />
<br />
<br />
|-<br />
|'''Project 65: [[Explorations in BioLuminescence]]'''<br />
[[File:bioluminescence.jpg|120px|left]]<br />
From fireflies to stinky squids... the future is bright<br />
<br />
<br />
|-<br />
|'''Project 66: [[Say Cheese]]'''<br />
[[File:Cheesemaking.jpg|120px|left]]<br />
make your own fresh cheese<br />
<br />
<br />
|-<br />
|'''Project 67: [[Hack a Taq]]'''<br />
[[File:Hack-a-taq.jpg|120px|left]]<br />
Testing out the Wild OpenPCR and purifying your own Taq from Open Biotechnology - using primers to detect coliform bacteria <br />
<br />
|-<br />
|'''Project 68: [[Poor Man's BioPrinter]]'''<br />
[[File:poorBioPrinter.jpg|120px|left]]<br />
There are many ways of bioprinting...<br />
<br />
|-<br />
|'''Project 69: [[DIYbio Prototypes Collection & Hackteria archive]]'''<br />
[[Image:2.jpg|120px|left]]<br />
Collection of open biology and DIYbio prototypes created by members of the Hackteria network, such as DNA Lab-in-Box, Gene gun etc. This archive of exhibition artefacts supports open science and citizen science advocacy and showcases the open hardware possibilities of supporting research in the Global South.<br />
<br />
|-<br />
|'''Project 70: [[Urban Cuisine]]'''<br />
[[Image:snail_huntin.jpg|120px|left]]<br />
Various explorations into urban ecologies and recipes to hunt, collect, grow, cook, eat your findings <br />
<br />
|-<br />
|'''Project 71: [[Actinomycetes Tournament: Open antibiotics discovery]]'''<br />
[[Image:acti.jpg|120px|left]]<br />
In this project we want to screen soil bacteria, such as Actinomyces, Streptomyces and Micromonospora for antibiotic properties. It is a global citizen science initiative started by DIYbio Waag, which uses game elements (collecting and tournaments) to support open drug discovery of antibiotics. We will isolate actinomycetes from soil samples around the world and screen with Micrococcus luteus or similar microbe to determine their antibiotic properties. Images of inoculated plates will be shared online and based on their strengths (zones of growth inhibition) they will become part of a collection or initiate tournaments between the most successful examples. We will sequence the most powerful ones. We expect cooperation with various DIYbio organizations, microbiology departments and science enthusiasts. <br />
Picture: Antibiotic Producing Actinomycetes By adonofrio (Biology101.org)<br />
<br />
|-<br />
|'''Project 72: [[High Voltage Epigenetics]]'''<br />
[[Image:high_voltage_yeast.jpg|120px|left]]<br />
Is it real or is it epigenetics? A novel method is described, which, on the basis of the short-term application of electrostatic fields, results in lasting beneficial and desirable properties in animals and plants. The culture of fish, particularly of edible fish but also of ornamental fish, is genuinely revolutionised. <br />
<br />
|-<br />
|'''Project 73: [[How to make a proper Barber Trap]]'''<br />
[[Image:BarberTrap.png|120px|left]]<br />
<br />
|-<br />
|'''Project 74: [[doms Mobile vj lab]]'''<br />
As time past I have grow huge need for mobile vj lab for all indoor and outdoor visual activity.<br />
From my experience till now it has to be paced with lots of power, projector, cams, microscope,sound system, bio lab tools (pipettes, slide glass...),many different vj tools(blinking led...), lasers, traps and containers for live organisms. <br />
Probably some other stuff that I will find useful like knife, glue, bugs 2d aquarium, different plugs for charging and powering<br />
that you always find helpful on terrain.<br />
Of course all together should not be to heavy (5kg max but rather 3kg)....<br />
... <br />
<br />
|-<br />
|'''Project 75: [[openTrap]]'''<br />
[[Image:FoxTrap.jpg|120px|left]]<br />
More and more we are looking into various traps for catching larger animals such as rats, foxes, pigeons and more. Re-developing some popular designs, adapting them, make them open source for digital fabrication<br />
... <br />
<br />
<br />
|-<br />
|'''Project 76: [[DIY_NanoDrop]]'''<br />
[[Image:DIYNanoDrop.jpg|120px|left]]<br />
Microvolume Spectrophotometers and Fluorospectrometers are innovative products used to quantitate micro-volumes of sample. You want one? Build your own.<br />
... <br />
<br />
<br />
|-<br />
|'''Project 77: [[Nail Studio Micro Fluidics]]'''<br />
[[Image:NailStudioMicroFluidics.jpg|120px|left]]<br />
Nail studios are the girls secret hight tech labs with UV light curing gel and other fancy stuff. Let's hack it to do low cost microfluidics.<br />
... <br />
<br />
|-<br />
|'''Project 78: [[Mobile Power Solutions]]'''<br />
[[Image:PowerSolutions.jpg|120px|left]]<br />
You need mobile electrical power - On how to build Graphene Supercapacitors, Peltier electricity from fire and more...<br />
<br />
|-<br />
|'''Project 79: [[Fish-Hacking]]'''<br />
[[Image:IMG_2593.JPG|120px|left]]<br />
Ever tried to listen into the electrocommunication behavior of weakly electric fish? Recent experiments and general info about black ghost knifefish, inspired by [http://hackteria.org/wiki/index.php/Fish-to-Brain_Interfaces,_Antony_Hall,_Ljubljana Tony Hall's work on ENKI technology]. They have also been shown to be useful for biomonitoring of pullutants in water! Fish-2-pd interfaces are already working nicely.<br />
<br />
|-<br />
|'''Project 80: [[Long Range Microphone for Cicadas & Cricket Hunting]]'''<br />
[[Image:Garengpung.jpg|120px|left]]<br />
Imagine you are in the forest, rivers, or mountains and some noises just appears and hunting your ears and you kind of wanted to be sure what is it and where is it come from. This long range microphone might help you to solve the mistery. But, as the title says it will be used for hunting cricket and cicadas a.k.a garengpung, then we must have some ideas about how the cricket and cicadas a.k.a garengpung sounds like. - still under construction. Hhaha... so is everything else on that wiki :-)<br />
<br />
|-<br />
|'''Project 81: [[DIY Microbial Fuel Cells]]'''<br />
[[Image:Mud_Battery.png|120px|left]]<br />
Imagine you are in the forest and create a bit of electricity from mud...<br />
<br />
|-<br />
|'''Project 82: [[Water Sampling Coconut]]'''<br />
[[Image:CoconutSensor.jpg|120px|left]]<br />
GPS and water sensors in a coconut go explore the rivers.<br />
<br />
|-<br />
|'''Project 83: [[Psychokinetic Action of Young Chicks]]'''<br />
[[Image:PsychoCkicks.jpg|120px|left]]<br />
What if there was such a thing as Psychokinetics - let's test. <br />
<br />
|-<br />
|'''Project 84: [[Aquatic Malaria Extinction Robot - TEAM1]]'''<br />
[[Image:Malaria_siech_dusjagr.jpg|120px|left]]<br />
Some thoughts and experiments about malaria reduction, mosquito killing and stinky socks<br />
<br />
|-<br />
|'''Project 85: [[SATW - Do-it-yourself von Laborgeräten in der Bioanalytik]]'''<br />
[[Image:SATW-DIY_kit.jpg|120px|left]]<br />
Co-Development of a workshop on DIY laboratory instruments for bioanalytics, colorimetry, low-cost microcontrollers and simple DIY electronics, enzymatic assays. With a concept of "teach the teachers", we are looking into DIY lab-tools as means of a pedagogic tool for interdisciplinary thinking at the biomedical/engineering/molecular interface. Coproduced with SATW, Swiss Academy of Technical Sciences, Hackteria and FHNW, School for Lifesciences.<br />
<br />
|-<br />
|'''Project 86: [[GynePUNK biolabs]]'''<br />
[[Image:smell_my_finger.jpg|120px|left]]<br />
key words: ANARCHA biopunk - DIY bodyaction - radical SelFpower - Analisis of fluids - self citology - DIY equipment<br />
<br />
BIO-reSEARCH is part of [[Pechblenda]] tentacles, mixed with [http://anarchagland.hotglue.me AnarchaGland] researchs and [http://gynepunk.tumblr.com/ GynePUNK biolabs]<br />
<br />
|-<br />
|'''Project 87: [[Ayam Cemani - ... or the Black Chicken Project]]'''<br />
[[Image:Ayam cemani kid.jpg|120px|left]]<br />
Ayam means "chicken" in Indonesian and Cemani is "completely black" in Javanese.<br />
<br />
|-<br />
|'''Project 88: [[Lick the Moonmilk]]'''<br />
[[Image:Mondmlichloch.png|120px|left]]<br />
Exploring the origin and use of moonmilk. Cave safari to the Moondmilchloch in Lucern.<br />
<br />
|-<br />
|'''Project 89: [[Elektrowetting]]'''<br />
[[Image:Elektrowetting.png|120px|left]]<br />
Playing with electro-wetting effect. Trying to finally make that desktop DNA synthesizer.<br />
<br />
|-<br />
|'''Project 90: [[Nucleic_Acid_Quantitation]]'''<br />
[[Image:nucleicAcidAbsorbtion.png|120px|left]]<br />
Nucleic_Acid_Quantitation<br />
<br />
|-<br />
|'''Project 91: [[Wild openQCM]]'''<br />
[[Image:wild_openQCM.jpg|120px|left]]<br />
Make your free open wild openQCM aka artificial nose<br />
<br />
|-<br />
|'''Project 92: [[CocoMake7]]'''<br />
[[Image:CocoMake7_boardShape.png|120px|left]]<br />
A jugaad ultra low-cost clone to the Makey Makey, but based on QTouchADC sensing, local manufacturing and coconuts<br />
<br />
|-<br />
|'''Project 93: [[Nano hacking science and DIY/DIWO low cost diagnosis]]'''<br />
[[Image:IMG_0845.png|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 94: [[MicroMacroPleasure]]'''<br />
[[Image:Ursspeculum.jpg|120px|left]]<br />
Dildomancy hacking science and DIY/DIWO low cost pleasure and vaginal trainning<br />
<br />
|-<br />
|'''Project 95: [[Biocomons.Lab]]'''<br />
[[Image:11738011_692237857544097_3209041620878190282_n.jpg|120px|left]]<br />
A meeting to define the bio-value and the bio-capital in the era of biotechnology. 6 days of unconferences and workshops to push further the [http://www.bio-commons.org/ Bio-Commons research project].<br />
<br />
|-<br />
|'''Project 96: [[Mobile Plant Analysis Lab @ pifCamp, Slovenia]]'''<br />
[[Image:DusjagrLab_pifCamp.jpg|120px|left]]<br />
PIFcamp - Summer Hacking Camp, 3 – 9 August 2015 @ Trenta, Slovenia<br />
<br />
|-<br />
|'''Project 97: [[Jugaad PhoneScope]]'''<br />
[[Image:Lens_closeUp.jpg|120px|left]]<br />
Very simple and self-made DIY lens to attach to your smart-phone and turning it into a nice microscope.<br />
<br />
|-<br />
|'''Project 98: [[openlab gynecologyDIT]]'''<br />
[[Image:Dr-harry-waites-vibrator.jpg|120px|left]]<br />
Residence Fablab PlateformeC [www.plateforme-c.org] One of the topics or lines to explore Antropocene Or not ! PLASTICOCENE<br />
<br />
|-<br />
|'''Project 99: [[Euglena Burger]]'''<br />
[[Image:japan_euglenaBurger.png|120px|left]]<br />
The Euglena Burger - half animal and half plant. finally working on the recipes, upscaling using custom made home bio-reactors and looking forward to serve it to our friends.<br />
<br />
|-<br />
|'''Project 100: [[Save the Whales]]'''<br />
[[Image:pandas_will_save_the_whales.jpg|120px|left]]<br />
No more words - We need ACTION!<br />
<br />
|-<br />
|'''Project 101: [[PCR your DNA Workshop]]'''<br />
[[Image:WildOpenPCR.jpg|120px|left]]<br />
All you need to prepare for a basic workshop on getting some DNA, setting up a PCR, and running the gel to see some results in a day-long workshop<br />
<br />
|-<br />
|'''Project 102: [[Worm Composting]]'''<br />
[[Image:Wormcompost_3months.jpeg|120px|left]]<br />
Indoors or outdoors, a great way to reduce your garbage amounts and get super-rich compost for the garden<br />
<br />
|-<br />
|'''Project 103: [[Open Source Estrogen]]'''<br />
[[File:PIIS001078241100179X.gr1.lrg.jpg|120px|left]]<br />
Community page for DIY estrogen!!!<br />
<br />
|-<br />
|'''Project 104: [[Feuerphilosophen]]'''<br />
[[File:Alchemy.jpg|120px|left]]<br />
Alchemy Reenactment Session<br />
<br />
<br />
|-<br />
|'''Project 105: [[Fostering Duckweed]]'''<br />
[[File:DSC01035.jpg|120px|left]]<br />
Duckweed might be the food of the future? Can we grow it from our own pee as fertilizer?<br />
<br />
<br />
|-<br />
|'''Project 106: [[Völlig Übertriibe]]'''<br />
[[File:Mark_aquarium.jpg|120px|left]]<br />
Session in Schaffhausen for exploring BioVJing and other crazy Hydra-hacks<br />
<br />
<br />
|-<br />
|'''Project 107: [[Laser-Technologie 2000]]'''<br />
[[File:LaserTechnologie_2000_circuit.png|120px|left]]<br />
simple constant current circuit for hacking lasers<br />
<br />
<br />
|-<br />
|'''Project 109: [[Chrisper-Chäsli]]'''<br />
[[File:DIY_CRISPR_Kits.jpg|120px|left]]<br />
Some reflections on gene-editing and making cheese<br />
<br />
<br />
|-<br />
|'''Project 110: [[PRG Ikan Hobi - GMO fish geeking]]'''<br />
[[File:PRK_ikan_Jan2017_lifepatch.jpg|120px|left]]<br />
Geeking around with zebra-fish, CRISPR and the GM-fish (formerly know as glow-fish) we found on the market in Yogyakarta. Seems the Indonesian regulations are supporting the use of GM fish for sports, hobby, crafts and ornamental use!<br />
<br />
<br />
|-<br />
|'''Project 111: [[DIY chemistry analysis of food]]'''<br />
[[File:CuvettesGrapesmall.jpg|120px|left]]<br />
Series of protocols in development for extracting and analysing micronutrients in foods, using DIY methods and without the need for lab access.<br />
<br />
<br />
|-<br />
|'''Project 112: [[Make you own CRISPR-Kit]]'''<br />
[[File:TheCRISPR.jpg|120px|left]]<br />
Creative workshop at Schloss Solitude. Design your own CRISPR-Kit.<br />
<br />
|-<br />
|'''Project 112: [[Miso]]'''<br />
[[File:Dplus_miso_mixSojaRice.jpg|120px|left]]<br />
Miso (味噌 or みそ) making Workshop at Dimension Plus TW<br />
<br />
|-<br />
|'''Project 113: [[DIY Single Photon Generator]]'''<br />
[[File:SinglePhoton.jpg|120px|left]]<br />
Finding a hack to generate single photons. Quantum-Nano-Dots, Cristals, Ions?<br />
<br />
|-<br />
|'''Project 114: [[Gär Lämpli]]'''<br />
[[File:GarLampli_box_Pink.jpg|120px|left]]<br />
Check the Temperature and Time profile of your ferments... with just 1 LED<br />
<br />
|-<br />
|'''Project 115: [[Back to Bombyx]]'''<br />
[[File:LiftOfSpiderPrint.JPG|120px|left]]<br />
Exploring silk, again.<br />
<br />
|-<br />
|'''Project 116: [[Circular Chromatography]]'''<br />
[[File:Circular_chromatography.jpg|120px|left]]<br />
Started at [http://wiki.artscienceblr.org/wiki/index.php/Circular_chromatography (Art)ScienceBLR's site], enjoy concentric separation of matter - from soil, and developing sugars<br />
<br />
|-<br />
|'''Project 117: [[DIY Mutagenesis]]'''<br />
[[File:Mutagenesis.png|120px|left]]<br />
à la ur-institute<br />
<br />
<br />
|-<br />
|'''Project 118: [[Trans Organs on a Chip]]'''<br />
[[File:Transorgans_v1.png|120px|left]]<br />
grow your own transorgans for fully (micro) fluid(ic) exchange!<br />
<br />
|-<br />
|'''Project 119: [[Participation in Decision Making by Members of DIY Communites]]'''<br />
[[File:39890639411_d41caf1935_k.jpg|120px|left]]<br />
Research project on the needs of the community for sucessful collaboration with institutions<br />
<br />
<br />
|-<br />
|'''Project 120: [[HiSeq2000_-_Next_Level_Hacking]]'''<br />
[[File:HiSeq.jpg|120px|left]]<br />
Let's hack that HiSeq Next Level Sequencing machine and make it open!<br />
<br />
|-<br />
|'''Project 121: [[MakeBlock Me High-Power Encoder Motor Driver]]'''<br />
[[File:|120px|left]]<br />
Open Leaking Stuff<br />
<br />
|-<br />
|'''Project 122: [[Slime Mould]]'''<br />
[[File:Slime_mould_in_situ.JPG|120px|left]]<br />
more than the traveling salesman's problem<br />
<br />
<br />
<!-- Projects end --></div>Derishushttp://www.hackteria.org/wiki/index.php?title=File:Slime_mould_in_situ.JPG&diff=28390File:Slime mould in situ.JPG2018-06-07T15:31:36Z<p>Derishus: </p>
<hr />
<div></div>Derishushttp://www.hackteria.org/wiki/index.php?title=Collection_of_DIY_Biology,_Open_Source_Art_Projects&diff=28389Collection of DIY Biology, Open Source Art Projects2018-06-07T15:30:11Z<p>Derishus: </p>
<hr />
<div><!-- Projects --><br />
<div class="alertBox1"><br />
{| class="alert"<br />
!Projects<br />
<br />
|-<br />
|'''Project 0: [[WetWare]]'''<br />
[[File:WetWare_s.jpg|120px|left]]<br />
Where to shop for materials<br />
<br />
|-<br />
|'''Project 1: [[The Bacterial Net- Microbial Telecommunications.]]'''<br />
[[Image:2.jpg|120px|left]]<br />
Quorum sensing is a type of decision-making process used by decentralized groups to coordinate behavior. Many species of bacteria use quorum sensing to coordinate their gene expression according to the local density of their population.The Bacterial Net attempts to transfer this decision making process over the Internet. Using Microcontollers and sensors to detect bacterial population and a public web service http://www.pachube.com that helps transfer sensor information. The Bacterial Net is an exploration in bringing the Internet into the Natural Ecosystem. <br />
<br />
|-<br />
|'''Project 2: [[Bioelectronix]]'''<br />
<br />
[[Image:bioelectronics_after_casting.jpg|120px|left]]<br />
<br />
Integrating electronics into biological culture devices needs different approaches. the use of silicone-rubbers for sealing off the wet/humid bio-part from the silicon circuits and sensors allows the making of hybrid bioelectronic devices. <br />
|-<br />
| '''Project 3: [[Synthetic Biology for Artists and Designers: A primer]]'''<br />
[[File:synbio_cover.jpg|120px|left]]<br />
An Illustrated [http://hackteria.org/wiki/images/a/a1/Handbook.pdf <strong>handbook</strong>] that shows you how to work with MIT's parts registry<br />
|-<br />
| '''Project 4: [[ArtScienceBangalore 2009-Synthetic Biology for Artists and Designers]]'''<br />
[[File:Whiteboard.jpg|120px|left]]<br />
<br />
see also the [http://prix2011.aec.at/winner/3128/ docu for Prix Ars Electronica]<br />
<br />
<br />
|-<br />
|'''Project 5: [[DIY microscopy]]'''<br />
[[File:cam_ani_sm.gif|left]]<br />
<br />
a small hack to the optics of a standard webcam with an adjustable focus-lens, allows to create video data, with a magnification of around 100 to 400x (depending on the model) at a working distance of a few mm. The highest magnifications can be achieved by inverting (putting upside down) the lens. Addition of good lighting by the use of leds allows to create images using a bright-field method (shine through the stuff) or dark field method (look at the reflections and scattering). <br />
<br />
|-<br />
|'''Project 6:[[DIY $25 Gel Box]]'''<br />
[[File:DSC 8050.jpg|120px|left]]<br />
Building your own Electrophoresis Gel Box is easy. Here's how you do it!<br />
<br />
|-<br />
| '''Project 7: [[bio habitats]]'''<br />
[[File:biohabitat.jpg|120px|left]]<br />
instead of using standard lab culture tools, such as flasks or petri-dishes, artists might want to design their own devices to culture and inhabitate living organisms and keep them alive. simple devices can be cast in silicone to incorporate all the necessary environemtal conditions for long time survival of microorganisms.<br />
<br />
|-<br />
| '''Project 8: [[DIY Microfluidics]]'''<br />
[[File:Microfluidicwikiimage.png|120px|left]]<br />
<br />
|-<br />
| '''Project 9: [[DIY Plant Tissue culture and Engineering]]'''<br />
[[File:plant_culture.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 10: [[Pd_microscope]]'''<br />
[[File:pd_microscope.png|120px|left]]<br />
Pd_microscope is the software package that you can use to control your DIY microscope. It's a work in progress<br />
<br />
|-<br />
| '''Project 11: [[DIY spectroscopy]]'''<br />
[[File:spektral_tag1.jpg|120px|left]]<br />
Spectroscopy means the analysis of natural phenomena according to its energy distribution. In case of the electromagnetic spectra, ranging from x-ray, visible light, infrated to radio frequencies, this can be used to analyse chemical compounds, follow biochemical reactions or get information of the composition of stars. This project tries utilize webcams, CD/DVD and some construction material, to get a spectral analysis of ultra-violett and visible light sources, which might be used to follow enzymatic and metabolic reactions in cultured microorganisms and bacteria.<br />
<br />
|-<br />
| '''Project 12: [[ArtScienceBangalore 2010-Synthetic Biology for Artists and Designers]]'''<br />
[[File:Asb_2010_group.jpg|120px|left]]<br />
A bunch of regular people with very little idea of Synthetic Biology but ready to pick up eveything about it.<br />
<br />
Download the nicely illustrated Comic Book on Synthetic Ecologies here [[File:IGEM_Comic_2010.pdf]]<br />
<br />
|-<br />
| '''Project 13: [[DIY handheld centrifuge]]'''<br />
[[File:HandHeldCent.jpg |120px|left]]<br />
<br />
|-<br />
| '''Project 14: [[DIY table top centrifuge]]'''<br />
[[File:Table_top.JPG|120px|left]]<br />
<br />
|-<br />
| '''Project 15: [[DIY Incubator]]'''<br />
[[File:DSC03096.JPG|120px|left]]<br />
<br />
<br />
|-<br />
| '''Project 16: [[DIY Fermentation or the Art of making Wine]]'''<br />
[[File:diy_fermentation.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 17: [[Hacked Optical Mouse]]'''<br />
[[File:optical_mouse_hack.jpg|120px|left]]<br />
<br />
|-<br />
| '''Project 18: [[DIY Sterlisation Hood]]'''<br />
[[File:DSC03946.JPG |120px|left]]<br />
<br />
|-<br />
| '''Project 19: [[DIY Water Bath]]'''<br />
[[File:DSC04040.JPG|120px|left]]<br />
<br />
|-<br />
| '''Project 20: [[GFP Protocol Jugaad]]'''<br />
[[File:MG_7283.JPG|120px|left]]<br />
Home made GFP bacteria<br />
<br />
|-<br />
| '''Project 21: [[ReacTable]]'''<br />
[[File:DSC04180.JPG|120px|left]]<br />
Gives information and protocals of equipment and bacteria put on it<br />
<br />
|-<br />
| '''Project 22: [[Algae Culture at Home]]'''<br />
[[File:Plankton.jpg|120px|left]]<br />
Grow your own algae at home with some plastic bottles, lighting and air bubbles<br />
<br />
|-<br />
| '''Project 23: [[Mobile Phone Field Microscope]]'''<br />
[[File:hacked_phone.jpg|120px|left]]<br />
Modify a mobile phone into a useful field microscope by replacing the lens with a cheap flipped wemcam lens..<br />
<br />
|-<br />
| '''Project 24: [[DIY turbidity meters]]'''<br />
[[File:turbidity_shield.jpg|120px|left]]<br />
simple light absorption measurements for water monitoring or nanoparticle detection using an arduino, a laser or LED and an LDR.<br />
<br />
|-<br />
| '''Project 25: [[Laser-cut microscopy stages]]'''<br />
[[File:miniScope.jpg|120px|left]]<br />
different design for easy to build stages for the DIY microscope, z-stage, x-y servo controlled stage and more...<br />
<br />
|-<br />
| '''Project 26: [[How to start a nanolab]]'''<br />
[[File:nanolab.jpg|120px|left]]<br />
Because nanotechnology is situated at the interface of many disciplines it is best to organize some basic gear from various sources, such as a local electronics store, gardening shops or various online suppliers for household tools and electronic gadgets. You will need to set up some basic laboratory instruments for doing simple biology, chemistry, fabrication&manufacturing and some physical computing to connect everything to your computer.<br />
<br />
|-<br />
| '''Project 27: [[Magnetic stirrer]]'''<br />
[[File:NS_spinner_spinning.jpg|120px|left]]<br />
always useful to stir things automatically if you are lazy...<br />
<br />
|-<br />
|'''Project 28: [[DIY Micro Dispensing and Bio Printing]]'''<br />
[[File:nanofab.jpg|120px|left]]<br />
dispensing, cutting, printing, growing with a robotic platform. Aka NanoFabrik<br />
<br />
|-<br />
|'''Project 29: [[ArtScienceBangalore 2011-Synthetic Biology for Artists and Designers]]'''<br />
[[File:2011artsciblr.jpg|120px|left]]<br />
[http://2011.igem.org/Team:ArtScienceBangalore Link to IGEM site]<br />
<br />
Download the nicely illustrated booklet on "Dirt" here [[File:dirt_IGEM2011.pdf]]<br />
<br />
|-<br />
|'''Project 30: [[ArtScienceBangalore 2011-Nanotechnology for Artists and Designers]]'''<br />
[[File:Mindmap1.JPG|120px|left]]<br />
[http://openwetware.org/wiki/Biomod/2011/SRISHTI/ArtScienceBangalore Link to Biomod site]<br />
<br />
|-<br />
|'''Project 31: [[TEMPE(h) : THE INSTRUCTIONS]]'''<br />
[[File:tempe3.jpg|120px|left]]<br />
Tempe(h) is a fermented food. But it's not meant to preserve the material from rottenness. It's for style. Analogue meat they said. So, once you made your own tempe(h). Cook it! Eat it! <br />
<br />
<br />
<br />
|-<br />
|'''Project 32: [[Daphniaology]]'''<br />
[[File:daphnia_jar.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 33: [[Ants]]'''<br />
[[File:and_lenoir.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 34: [[Book: Homemade Biological Art]]'''<br />
[[File:homemade_BioArt.jpg|120px|left]]<br />
<br />
|-<br />
|'''Project 35: [[The Art of Hunting]]'''<br />
[[File:hunter.jpg|120px|left]]<br />
Baerman funnels, Berlese-Tullgren funnel, habitats<br />
<br />
|-<br />
|'''Project 36: [[Jugaad PCR Thermocyclers]]'''<br />
[[File:Hairdryer_PCR_lab2011.jpg|120px|left]]<br />
some links to alternative and DIY thermocycling experiments.<br />
<br />
|-<br />
|'''Project 37: [[MobileLabs]]'''<br />
[[File:mobilelabs.jpg|120px|left]]<br />
some links and ideas to mobile labs...<br />
<br />
|-<br />
|'''Project 38: [[Autonomous Public Lab]]'''<br />
[[File:Buildingabiodome.jpg|120px|left]]<br />
Blueprints for setting up a People's Research Lab<br />
<br />
|-<br />
|'''Project 39: [[Hackteria Merchandise and Logos]]'''<br />
[[File:Biohacker.png|120px|left]]<br />
loads of logos, t-shirt designs, stickers, flyers and posters<br />
<br />
|-<br />
|'''Project 40: [[Orbital Shaker]]'''<br />
[[File:orbital_shaker.jpg|120px|left]]<br />
shake it baby!<br />
<br />
|-<br />
|'''Project 41: [[Plant Electrophysiology]]'''<br />
[[File:web9.jpg|120px|left]]<br />
Yeah, even plants cant be protected from arduinos..<br />
<br />
|-<br />
|'''Project 42: [[Spin Coater]]'''<br />
[[File:spin_coater.jpg|120px|left]]<br />
DIY Spin Coater!<br />
<br />
|-<br />
|'''Project 43: [[NeuroNetworking]]'''<br />
[[File:dkbrain.jpg|120px|left]]<br />
Prototypes & design fiction into networking over brain data and biodata related to our brain function (fMRI, SNPs, EEG).<br />
How will we use brain data for social networking, neuromarketing, citizen science projects,etc.<br />
The main technical challenge is to create some open format for sharing brain data over the web (3D T1-weighted gradient-echo sequence (MP-RAGE)images in the nifti format http://nifti.nimh.nih.gov/nifti-1 , which is readable by FSL software (http://www.fmrib.ox.ac.uk/fsl/ into some 3D web browser data and image that would support crowdsourcing of brain data, pattern recognition and comparison of brain data).<br />
<br />
|-<br />
|'''Project 44: [[Garden 2012: Spherification of Hieronymus Bosch]]'''<br />
[[File:Spheres_big.jpg|120px|left]]<br />
Spherification experiments as a tribute to Hieronymus Bosch's Garden of Earthly Delights. In order to understand our new cosmology of minimal but also complex spheres (molecules, cells, vesicles, coacervates, microspheres), we decided to use spherification techniques and to try to design a version of Bosch's Garden of Earthly Delights like a catalogue of what is possible in terms of spheres today. Some of our spheres are eatable, other are perceived only by microscopes, some target taste buds other just molecules, we even tried to prepare a micro-meal for microorganisms entrapped in a type of mini-aquaria and systems. The three containers mimicking the original triptych are an early reflection of the importance of spheres for our idea of origin or the organic life but also present experiments in molecular gastronomy but also drug targeting systems and research in nano-bio technologies. Humans making spheres on all scales are like gods recreating some new garden with self-enclosed systems, ecologies and microspheres, which influence each other and interact.<br />
<br />
|-<br />
|'''Project 45: [[Bio+Hacker+Art-Space Tour California 2012]]'''<br />
[[File:cal_local.jpg|120px|left]]<br />
Picture protocoll of an intensive three week travel throug the venues of third culture in western California!<br />
<br />
<br />
|-<br />
|'''Project 46: [[Distillation]]'''<br />
[[File:distillation.jpg|120px|left]]<br />
Distillation at the Hackteria Distillery at Jagaa, Bangalore Dec. 2012 and other distillation rigs<br />
<br />
|-<br />
|'''Project 47: [[Do eat your laser Tofu (DEYLT)]]'''<br />
[[File:tof_icon.jpg|120px|left]]<br />
How to make your own tofu? From laser cutting a tofu mold to the preparation and the tasting.<br />
<br />
|-<br />
|'''Project 48: [[Randen-Burger or the art of documenting]]'''<br />
[[File:randen.jpg|120px|left]]<br />
...<br />
<br />
|-<br />
|'''Project 49: [[Hackteria_%26_SGMK_BioCyberKidzz#Yeast_powered_balloons|Yeast powered balloons a la BioCyberKidzz]]'''<br />
[[File:Maxiblasinator.jpg|120px|left]]<br />
This is a balloon, which inflates as a result of the yeast growing and interacting with its environment <br />
<br />
|-<br />
|'''Project 50: [[Hackteria_%26_SGMK_BioCyberKidzz#Bacterial_jewelery|Bacterial Jewelry a la BioCyberKidzz]]'''<br />
[[File:jewelry.jpg|120px|left]]<br />
The Bacterial Jewelry module comes as a take away amulet that has a living fingerprint of the participant. <br />
<br />
<br />
|-<br />
|'''Project 51: [[Hackteria_%26_SGMK_BioCyberKidzz#Body_Augmentation_and_Enhancement|Body Augmentation and Enhancement a la BioCyberKidzz]]'''<br />
[[File:elephantear.jpg|120px|left]]<br />
Talking about bioart and cyberbody modifications is accessible to everyone. <br />
<br />
|-<br />
|'''Project 52: [[Hackteria_%26_SGMK_BioCyberKidzz#LabBook|The LAB BOOK a la BioCyberKidzz]]'''<br />
[[File:laborbuch2.jpg|120px|left]]<br />
Recording observations is an important aspect of laboratory life. This module is to encourage the collection and recording of data. <br />
<br />
|-<br />
|'''Project 53: [[DIY Laser tweezer, cell trap, oligo synthesis]]'''<br />
[[File:tweez.png|120px|left]]<br />
Based on a paper of a laser tweezer made from an old DVD drive we go on...<br />
<br />
<br />
|-<br />
|'''Project 54: [[DIY Plasma generators]]'''<br />
[[File:plasma_siech.jpg|120px|left]]<br />
Where to get it, how to make it, the 4th state of matter.<br />
<br />
|-<br />
|'''Project 55: [[Hacked Hard-disc Centrifuge]]'''<br />
[[File:harddisc_centrifuge.jpg|120px|left]]<br />
Get an old harddisc, laser cut or 3D pring a eppi-holder, run it...<br />
<br />
|-<br />
|'''Project 56: [[Agar is the Media]]'''<br />
[[File:agar_media.jpg|120px|left]]<br />
The key technologies of every genetic and molecular biology lab – the gel electrophoresis chamber...<br />
<br />
<br />
|-<br />
|'''Project 57: [[BubbleBots]]'''<br />
[[File:BubbleBots.jpg|120px|left]]<br />
Tiny little tubular rockets generating bubbles to propel themselves through water.<br />
<br />
|-<br />
|'''Project 58: [[Ganzfeld experiment]]'''<br />
[[File:Ganzfeld experiment.JPG|120px|left]]<br />
This is a modified experiment from the 70s and which can be used as a great icebreaker activity for building trust.<br />
<br />
|-<br />
|'''Project 59: [[Rubber_Hand_Illusion_Experiment_variations]]'''<br />
[[File:Wooden-hand.jpeg|120px|left]]<br />
a variation developed through trying this workshop on a large number of people, and not having funding to get loads of rubber hands. So we made the hands in the workshop.<br />
<br />
|-<br />
|'''Project 60: [[Internet of Bugs'n'Beasts]]'''<br />
[[File:bugsnbug.jpeg|120px|left]]<br />
starting soon<br />
<br />
|-<br />
|'''Project 61: [[KIMCHI or the Art of Fermentation]]'''<br />
[[File:kimchi01.jpg|120px|left]]<br />
HomeMade KIMCHI from scratch.....<br />
The final taste all depends on the amount of salt, dehydration, fermentation and temperature monitor.<br />
<br />
<br />
<br />
|-<br />
|'''Project 62: [[Science For Poets]]'''<br />
[[File:Webbana.jpg|120px|left]]<br />
LifeSciences Workshop for Artists & Designers in Bangalore 2013, collaborators of biodesign.cc<br />
<br />
|-<br />
|'''Project 63: [[Zebrafish Hacking: The Plant Animal]]'''<br />
[[File:ZEBRAFISH.jpg|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 64: [[Wild OpenPCR]]'''<br />
[[File:WildOpenPCR.JPG|120px|left]]<br />
Building an OpenPCR without the kit, just from commercially available parts. With additional information on how to do it.<br />
<br />
<br />
|-<br />
|'''Project 65: [[Explorations in BioLuminescence]]'''<br />
[[File:bioluminescence.jpg|120px|left]]<br />
From fireflies to stinky squids... the future is bright<br />
<br />
<br />
|-<br />
|'''Project 66: [[Say Cheese]]'''<br />
[[File:Cheesemaking.jpg|120px|left]]<br />
make your own fresh cheese<br />
<br />
<br />
|-<br />
|'''Project 67: [[Hack a Taq]]'''<br />
[[File:Hack-a-taq.jpg|120px|left]]<br />
Testing out the Wild OpenPCR and purifying your own Taq from Open Biotechnology - using primers to detect coliform bacteria <br />
<br />
|-<br />
|'''Project 68: [[Poor Man's BioPrinter]]'''<br />
[[File:poorBioPrinter.jpg|120px|left]]<br />
There are many ways of bioprinting...<br />
<br />
|-<br />
|'''Project 69: [[DIYbio Prototypes Collection & Hackteria archive]]'''<br />
[[Image:2.jpg|120px|left]]<br />
Collection of open biology and DIYbio prototypes created by members of the Hackteria network, such as DNA Lab-in-Box, Gene gun etc. This archive of exhibition artefacts supports open science and citizen science advocacy and showcases the open hardware possibilities of supporting research in the Global South.<br />
<br />
|-<br />
|'''Project 70: [[Urban Cuisine]]'''<br />
[[Image:snail_huntin.jpg|120px|left]]<br />
Various explorations into urban ecologies and recipes to hunt, collect, grow, cook, eat your findings <br />
<br />
|-<br />
|'''Project 71: [[Actinomycetes Tournament: Open antibiotics discovery]]'''<br />
[[Image:acti.jpg|120px|left]]<br />
In this project we want to screen soil bacteria, such as Actinomyces, Streptomyces and Micromonospora for antibiotic properties. It is a global citizen science initiative started by DIYbio Waag, which uses game elements (collecting and tournaments) to support open drug discovery of antibiotics. We will isolate actinomycetes from soil samples around the world and screen with Micrococcus luteus or similar microbe to determine their antibiotic properties. Images of inoculated plates will be shared online and based on their strengths (zones of growth inhibition) they will become part of a collection or initiate tournaments between the most successful examples. We will sequence the most powerful ones. We expect cooperation with various DIYbio organizations, microbiology departments and science enthusiasts. <br />
Picture: Antibiotic Producing Actinomycetes By adonofrio (Biology101.org)<br />
<br />
|-<br />
|'''Project 72: [[High Voltage Epigenetics]]'''<br />
[[Image:high_voltage_yeast.jpg|120px|left]]<br />
Is it real or is it epigenetics? A novel method is described, which, on the basis of the short-term application of electrostatic fields, results in lasting beneficial and desirable properties in animals and plants. The culture of fish, particularly of edible fish but also of ornamental fish, is genuinely revolutionised. <br />
<br />
|-<br />
|'''Project 73: [[How to make a proper Barber Trap]]'''<br />
[[Image:BarberTrap.png|120px|left]]<br />
<br />
|-<br />
|'''Project 74: [[doms Mobile vj lab]]'''<br />
As time past I have grow huge need for mobile vj lab for all indoor and outdoor visual activity.<br />
From my experience till now it has to be paced with lots of power, projector, cams, microscope,sound system, bio lab tools (pipettes, slide glass...),many different vj tools(blinking led...), lasers, traps and containers for live organisms. <br />
Probably some other stuff that I will find useful like knife, glue, bugs 2d aquarium, different plugs for charging and powering<br />
that you always find helpful on terrain.<br />
Of course all together should not be to heavy (5kg max but rather 3kg)....<br />
... <br />
<br />
|-<br />
|'''Project 75: [[openTrap]]'''<br />
[[Image:FoxTrap.jpg|120px|left]]<br />
More and more we are looking into various traps for catching larger animals such as rats, foxes, pigeons and more. Re-developing some popular designs, adapting them, make them open source for digital fabrication<br />
... <br />
<br />
<br />
|-<br />
|'''Project 76: [[DIY_NanoDrop]]'''<br />
[[Image:DIYNanoDrop.jpg|120px|left]]<br />
Microvolume Spectrophotometers and Fluorospectrometers are innovative products used to quantitate micro-volumes of sample. You want one? Build your own.<br />
... <br />
<br />
<br />
|-<br />
|'''Project 77: [[Nail Studio Micro Fluidics]]'''<br />
[[Image:NailStudioMicroFluidics.jpg|120px|left]]<br />
Nail studios are the girls secret hight tech labs with UV light curing gel and other fancy stuff. Let's hack it to do low cost microfluidics.<br />
... <br />
<br />
|-<br />
|'''Project 78: [[Mobile Power Solutions]]'''<br />
[[Image:PowerSolutions.jpg|120px|left]]<br />
You need mobile electrical power - On how to build Graphene Supercapacitors, Peltier electricity from fire and more...<br />
<br />
|-<br />
|'''Project 79: [[Fish-Hacking]]'''<br />
[[Image:IMG_2593.JPG|120px|left]]<br />
Ever tried to listen into the electrocommunication behavior of weakly electric fish? Recent experiments and general info about black ghost knifefish, inspired by [http://hackteria.org/wiki/index.php/Fish-to-Brain_Interfaces,_Antony_Hall,_Ljubljana Tony Hall's work on ENKI technology]. They have also been shown to be useful for biomonitoring of pullutants in water! Fish-2-pd interfaces are already working nicely.<br />
<br />
|-<br />
|'''Project 80: [[Long Range Microphone for Cicadas & Cricket Hunting]]'''<br />
[[Image:Garengpung.jpg|120px|left]]<br />
Imagine you are in the forest, rivers, or mountains and some noises just appears and hunting your ears and you kind of wanted to be sure what is it and where is it come from. This long range microphone might help you to solve the mistery. But, as the title says it will be used for hunting cricket and cicadas a.k.a garengpung, then we must have some ideas about how the cricket and cicadas a.k.a garengpung sounds like. - still under construction. Hhaha... so is everything else on that wiki :-)<br />
<br />
|-<br />
|'''Project 81: [[DIY Microbial Fuel Cells]]'''<br />
[[Image:Mud_Battery.png|120px|left]]<br />
Imagine you are in the forest and create a bit of electricity from mud...<br />
<br />
|-<br />
|'''Project 82: [[Water Sampling Coconut]]'''<br />
[[Image:CoconutSensor.jpg|120px|left]]<br />
GPS and water sensors in a coconut go explore the rivers.<br />
<br />
|-<br />
|'''Project 83: [[Psychokinetic Action of Young Chicks]]'''<br />
[[Image:PsychoCkicks.jpg|120px|left]]<br />
What if there was such a thing as Psychokinetics - let's test. <br />
<br />
|-<br />
|'''Project 84: [[Aquatic Malaria Extinction Robot - TEAM1]]'''<br />
[[Image:Malaria_siech_dusjagr.jpg|120px|left]]<br />
Some thoughts and experiments about malaria reduction, mosquito killing and stinky socks<br />
<br />
|-<br />
|'''Project 85: [[SATW - Do-it-yourself von Laborgeräten in der Bioanalytik]]'''<br />
[[Image:SATW-DIY_kit.jpg|120px|left]]<br />
Co-Development of a workshop on DIY laboratory instruments for bioanalytics, colorimetry, low-cost microcontrollers and simple DIY electronics, enzymatic assays. With a concept of "teach the teachers", we are looking into DIY lab-tools as means of a pedagogic tool for interdisciplinary thinking at the biomedical/engineering/molecular interface. Coproduced with SATW, Swiss Academy of Technical Sciences, Hackteria and FHNW, School for Lifesciences.<br />
<br />
|-<br />
|'''Project 86: [[GynePUNK biolabs]]'''<br />
[[Image:smell_my_finger.jpg|120px|left]]<br />
key words: ANARCHA biopunk - DIY bodyaction - radical SelFpower - Analisis of fluids - self citology - DIY equipment<br />
<br />
BIO-reSEARCH is part of [[Pechblenda]] tentacles, mixed with [http://anarchagland.hotglue.me AnarchaGland] researchs and [http://gynepunk.tumblr.com/ GynePUNK biolabs]<br />
<br />
|-<br />
|'''Project 87: [[Ayam Cemani - ... or the Black Chicken Project]]'''<br />
[[Image:Ayam cemani kid.jpg|120px|left]]<br />
Ayam means "chicken" in Indonesian and Cemani is "completely black" in Javanese.<br />
<br />
|-<br />
|'''Project 88: [[Lick the Moonmilk]]'''<br />
[[Image:Mondmlichloch.png|120px|left]]<br />
Exploring the origin and use of moonmilk. Cave safari to the Moondmilchloch in Lucern.<br />
<br />
|-<br />
|'''Project 89: [[Elektrowetting]]'''<br />
[[Image:Elektrowetting.png|120px|left]]<br />
Playing with electro-wetting effect. Trying to finally make that desktop DNA synthesizer.<br />
<br />
|-<br />
|'''Project 90: [[Nucleic_Acid_Quantitation]]'''<br />
[[Image:nucleicAcidAbsorbtion.png|120px|left]]<br />
Nucleic_Acid_Quantitation<br />
<br />
|-<br />
|'''Project 91: [[Wild openQCM]]'''<br />
[[Image:wild_openQCM.jpg|120px|left]]<br />
Make your free open wild openQCM aka artificial nose<br />
<br />
|-<br />
|'''Project 92: [[CocoMake7]]'''<br />
[[Image:CocoMake7_boardShape.png|120px|left]]<br />
A jugaad ultra low-cost clone to the Makey Makey, but based on QTouchADC sensing, local manufacturing and coconuts<br />
<br />
|-<br />
|'''Project 93: [[Nano hacking science and DIY/DIWO low cost diagnosis]]'''<br />
[[Image:IMG_0845.png|120px|left]]<br />
<br />
<br />
|-<br />
|'''Project 94: [[MicroMacroPleasure]]'''<br />
[[Image:Ursspeculum.jpg|120px|left]]<br />
Dildomancy hacking science and DIY/DIWO low cost pleasure and vaginal trainning<br />
<br />
|-<br />
|'''Project 95: [[Biocomons.Lab]]'''<br />
[[Image:11738011_692237857544097_3209041620878190282_n.jpg|120px|left]]<br />
A meeting to define the bio-value and the bio-capital in the era of biotechnology. 6 days of unconferences and workshops to push further the [http://www.bio-commons.org/ Bio-Commons research project].<br />
<br />
|-<br />
|'''Project 96: [[Mobile Plant Analysis Lab @ pifCamp, Slovenia]]'''<br />
[[Image:DusjagrLab_pifCamp.jpg|120px|left]]<br />
PIFcamp - Summer Hacking Camp, 3 – 9 August 2015 @ Trenta, Slovenia<br />
<br />
|-<br />
|'''Project 97: [[Jugaad PhoneScope]]'''<br />
[[Image:Lens_closeUp.jpg|120px|left]]<br />
Very simple and self-made DIY lens to attach to your smart-phone and turning it into a nice microscope.<br />
<br />
|-<br />
|'''Project 98: [[openlab gynecologyDIT]]'''<br />
[[Image:Dr-harry-waites-vibrator.jpg|120px|left]]<br />
Residence Fablab PlateformeC [www.plateforme-c.org] One of the topics or lines to explore Antropocene Or not ! PLASTICOCENE<br />
<br />
|-<br />
|'''Project 99: [[Euglena Burger]]'''<br />
[[Image:japan_euglenaBurger.png|120px|left]]<br />
The Euglena Burger - half animal and half plant. finally working on the recipes, upscaling using custom made home bio-reactors and looking forward to serve it to our friends.<br />
<br />
|-<br />
|'''Project 100: [[Save the Whales]]'''<br />
[[Image:pandas_will_save_the_whales.jpg|120px|left]]<br />
No more words - We need ACTION!<br />
<br />
|-<br />
|'''Project 101: [[PCR your DNA Workshop]]'''<br />
[[Image:WildOpenPCR.jpg|120px|left]]<br />
All you need to prepare for a basic workshop on getting some DNA, setting up a PCR, and running the gel to see some results in a day-long workshop<br />
<br />
|-<br />
|'''Project 102: [[Worm Composting]]'''<br />
[[Image:Wormcompost_3months.jpeg|120px|left]]<br />
Indoors or outdoors, a great way to reduce your garbage amounts and get super-rich compost for the garden<br />
<br />
|-<br />
|'''Project 103: [[Open Source Estrogen]]'''<br />
[[File:PIIS001078241100179X.gr1.lrg.jpg|120px|left]]<br />
Community page for DIY estrogen!!!<br />
<br />
|-<br />
|'''Project 104: [[Feuerphilosophen]]'''<br />
[[File:Alchemy.jpg|120px|left]]<br />
Alchemy Reenactment Session<br />
<br />
<br />
|-<br />
|'''Project 105: [[Fostering Duckweed]]'''<br />
[[File:DSC01035.jpg|120px|left]]<br />
Duckweed might be the food of the future? Can we grow it from our own pee as fertilizer?<br />
<br />
<br />
|-<br />
|'''Project 106: [[Völlig Übertriibe]]'''<br />
[[File:Mark_aquarium.jpg|120px|left]]<br />
Session in Schaffhausen for exploring BioVJing and other crazy Hydra-hacks<br />
<br />
<br />
|-<br />
|'''Project 107: [[Laser-Technologie 2000]]'''<br />
[[File:LaserTechnologie_2000_circuit.png|120px|left]]<br />
simple constant current circuit for hacking lasers<br />
<br />
<br />
|-<br />
|'''Project 109: [[Chrisper-Chäsli]]'''<br />
[[File:DIY_CRISPR_Kits.jpg|120px|left]]<br />
Some reflections on gene-editing and making cheese<br />
<br />
<br />
|-<br />
|'''Project 110: [[PRG Ikan Hobi - GMO fish geeking]]'''<br />
[[File:PRK_ikan_Jan2017_lifepatch.jpg|120px|left]]<br />
Geeking around with zebra-fish, CRISPR and the GM-fish (formerly know as glow-fish) we found on the market in Yogyakarta. Seems the Indonesian regulations are supporting the use of GM fish for sports, hobby, crafts and ornamental use!<br />
<br />
<br />
|-<br />
|'''Project 111: [[DIY chemistry analysis of food]]'''<br />
[[File:CuvettesGrapesmall.jpg|120px|left]]<br />
Series of protocols in development for extracting and analysing micronutrients in foods, using DIY methods and without the need for lab access.<br />
<br />
<br />
|-<br />
|'''Project 112: [[Make you own CRISPR-Kit]]'''<br />
[[File:TheCRISPR.jpg|120px|left]]<br />
Creative workshop at Schloss Solitude. Design your own CRISPR-Kit.<br />
<br />
|-<br />
|'''Project 112: [[Miso]]'''<br />
[[File:Dplus_miso_mixSojaRice.jpg|120px|left]]<br />
Miso (味噌 or みそ) making Workshop at Dimension Plus TW<br />
<br />
|-<br />
|'''Project 113: [[DIY Single Photon Generator]]'''<br />
[[File:SinglePhoton.jpg|120px|left]]<br />
Finding a hack to generate single photons. Quantum-Nano-Dots, Cristals, Ions?<br />
<br />
|-<br />
|'''Project 114: [[Gär Lämpli]]'''<br />
[[File:GarLampli_box_Pink.jpg|120px|left]]<br />
Check the Temperature and Time profile of your ferments... with just 1 LED<br />
<br />
|-<br />
|'''Project 115: [[Back to Bombyx]]'''<br />
[[File:LiftOfSpiderPrint.JPG|120px|left]]<br />
Exploring silk, again.<br />
<br />
|-<br />
|'''Project 116: [[Circular Chromatography]]'''<br />
[[File:Circular_chromatography.jpg|120px|left]]<br />
Started at [http://wiki.artscienceblr.org/wiki/index.php/Circular_chromatography (Art)ScienceBLR's site], enjoy concentric separation of matter - from soil, and developing sugars<br />
<br />
|-<br />
|'''Project 117: [[DIY Mutagenesis]]'''<br />
[[File:Mutagenesis.png|120px|left]]<br />
à la ur-institute<br />
<br />
<br />
|-<br />
|'''Project 118: [[Trans Organs on a Chip]]'''<br />
[[File:Transorgans_v1.png|120px|left]]<br />
grow your own transorgans for fully (micro) fluid(ic) exchange!<br />
<br />
|-<br />
|'''Project 119: [[Participation in Decision Making by Members of DIY Communites]]'''<br />
[[File:39890639411_d41caf1935_k.jpg|120px|left]]<br />
Research project on the needs of the community for sucessful collaboration with institutions<br />
<br />
<br />
|-<br />
|'''Project 120: [[HiSeq2000_-_Next_Level_Hacking]]'''<br />
[[File:HiSeq.jpg|120px|left]]<br />
Let's hack that HiSeq Next Level Sequencing machine and make it open!<br />
<br />
|-<br />
|'''Project 121: [[MakeBlock Me High-Power Encoder Motor Driver]]'''<br />
[[File:|120px|left]]<br />
Open Leaking Stuff<br />
<br />
|-<br />
|'''Project 122: [[Slime Mould]]'''<br />
[[File:HiSeq.jpg|120px|left]]<br />
more than the traveling salesman's problem<br />
<br />
<br />
<!-- Projects end --></div>Derishushttp://www.hackteria.org/wiki/index.php?title=HiSeq2000_-_Next_Level_Hacking&diff=28145HiSeq2000 - Next Level Hacking2018-04-15T17:34:37Z<p>Derishus: /* Links and Information */</p>
<hr />
<div>[[File:HiSeq.jpg|400px]]<br />
<br />
We got a HiSeq 2000, Next Level Sequencing Machine from the Genomics Facility of Department of Biosystems Science and Engineering in Basel. Contact through Biozentrum, University of Basel. We got it for free with the only disclaimer: "The biohackers should understand that they are responsible to organize and pay for the transport as well as that there is no warranty or support that can be given neither by us nor the DBSSE." <br><br />
<br><br />
This type of machine seems to be quite difficult to get up and running and also reagents, flowcell-kits and software licences can be expensive. Since more of these machines seem to show up in second hand (there are new machine generations by Illumina) it would be worth trying to find a way to make them work. Sequencing for all.<br><br />
<br><br />
Specifications:<br><br />
https://www.illumina.com/documents/products/datasheets/datasheet_hiseq2000.pdf<br />
<br><br />
The HiSeq2000 (200Gb) was introduced in the year 2010. Followed by HiSeq2500 (500Gb) in 2012. And HiSeq X Ten (1000Gb) in 2014. In 2017 the NovaSeq series of machines was launched.<br><br />
The machine is a quite early on, from March 2011, Serial Number is 700792, so the machine can not be updated to software and chemistry v4. Only machines with SN# <br />
7001403 or higher can get the FPGA [https://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/upgrades/hiseq_v4_upgrade_information_sheet_140328_3.pdf update v4].<br><br />
[[File:27747171_1313614108739672_1344364391_o.jpg|100px]]<br />
<br><br />
<br><br />
=== First Inspection ===<br />
I made a first inspection on the machine. It seems very well made (2011). I still think it would be cool to make it run as is. It's basically a big microfluidic system. So if we get the pumps and the cameras to work we can hack it into anything we want 🙂. Even if it's not for sequencing - it's basically a holder for flow-cells with a fluorescence camera attached to it. And 32 channels with pumps and selector valves that attach to the flow cells. Plus a fridge and a computer.<br />
And peltier for heating and cooling (pcr). Now trying to get the control software. I also think the system is "relatively" open... the software can be downloaded and kind of installs, there is no ID checking on the supplies or anything. Looks very hackable. Also all the cases can be opened easily. Let's do a weekend hack-session on it.<br><br />
<br><br />
I think it's great opportunity to learn about next level sequencing and about how theses machines work.<br><br />
<br><br />
Fluidic System:<br><br />
[[File:27356260_1310155019085581_6425750036040119481_o.jpg|600px]]<br />
<br><br><br />
Some pictures from the inside of the machine:<br><br />
<gallery><br />
File:IMG_20180201_155204.jpg<br />
File:IMG_20180201_155208.jpg<br />
File:IMG_20180201_155416.jpg<br />
File:IMG_20180201_211136.jpg<br />
File:IMG_20180201_211144.jpg<br />
File:IMG_20180201_211925.jpg<br />
File:IMG_20180201_212240.jpg<br />
File:IMG_20180201_212251.jpg<br />
File:IMG_20180201_212316.jpg<br />
File:IMG_20180201_212444.jpg<br />
File:IMG_20180201_212453.jpg<br />
File:IMG_20180201_213250.jpg<br />
File:IMG_20180201_213258.jpg<br />
File:IMG_20180201_214433.jpg<br />
File:IMG_20180201_221007.jpg<br />
File:IMG_20180201_221216.jpg<br />
File:IMG_20180201_221229.jpg<br />
File:IMG_20180201_221420.jpg<br />
File:IMG_20180201_221434.jpg<br />
File:IMG_20180201_221528.jpg<br />
File:IMG_20180201_222654.jpg<br />
File:IMG_20180201_223556.jpg<br />
File:IMG_20180202_085730.jpg<br />
File:IMG_20180202_102732.jpg<br />
File:29693570_1362013383899744_1938193920_o.jpg<br />
File:29746581_1362015877232828_851304411_o.jpg<br />
</gallery><br />
<br />
<br />
Lausanne Bio-Hackerspace Hackuarium got a HiSeq2000 (SN# 700918) and Gustavo dissected it. Here some pictures that Rachel sent me with comments form what I think components are:<br />
<br />
[[File:HiSeqDisection.jpg|300px]][[File:HiSeqDisection2.jpg|300px]]<br><br><br />
<br />
=== Chemistry ===<br />
Some images describing the Illumina Next-Generation Sequencing Chemistry:<br><br />
[[File:Chemistry.png|400px]]<br />
<gallery><br />
File:figure1.png<br />
File:ilmn-step7-12.jpg<br />
File:reversible-terminator-sequencing-large.png<br />
</gallery><br />
[[File:HiSeqProcess.jpg|480px]]<br><br />
Illumina uses a process called "Sequencing-by-synthesis"<br><br />
The HiSeq (and MiSeq) use 4-colour SBS<br><br />
<br><br><br />
The full DNA to Data solution:<br><br />
[[File:Process.png|600px]]<br />
<br><br><br />
Paper on the 4 color SBS:<br><br />
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1702316/<br />
<br><br><br />
Video with the possibilities for library preparation:<br><br />
https://www.youtube.com/watch?v=_yC0Bzw3WbQ<br><br />
Library prep kit with sample purification by magnetic beads:<br><br />
https://www.youtube.com/watch?v=UE1TAZZPUFI<br />
<br><br><br />
The CBot 2 System is used to prepare the clusters on the flow cell:<br><br />
https://emea.illumina.com/products/by-type/accessory-products/cbot.html<br />
<br><br><br />
HiSeq (and TrueSeq) Rapid Cluster Kits can be clustered on the HiSeq (on-board cluster). They also seem to have a lower read and are cheaper:<br><br />
https://emea.illumina.com/products/by-type/sequencing-kits/cluster-gen-sequencing-reagents/hiseq-rapid-cluster-kit-pe-sr.html<br />
<br><br><br />
===== Chemisty Kits Option (running on our machine): =====<br />
<gallery><br />
File:hiseq-rapid-cluster-kit-pe-sr.jpg<br />
File:hiseq-rapid-sbs-kit.jpg<br />
</gallery><br />
<br />
All Library Prep Kits<br><br />
Cluster Generation (cBot needed):<br />
* TruSeq SR Cluster Kit v3 - cBot-HS - Price: 4360 CHF<br />
* TruSeq PE Cluster Kit v3 - cBot-HS - Price: 6674 CHF<br />
* TruSeq Rapid SR and PE Cluster Kits, cBot Duo Cluster Kit - Price: Request<br />
Maybe not compatible (for reference):<br><br />
* HiSeq SR Rapid Cluster Kit v2, Price: 1002 CHF<br />
* HiSeq PE Rapid Cluster Kit v2, Price: 1540 CHF<br />
<br />
SBS Reagents:<br />
* TruSeq SBS Kit v3 - HS (50 cycles), Price: 2552 CHF<br />
* TruSeq Rapid SBS Kit - HS, Price: Request<br />
Maybe not compatible (for reference):<br><br />
*HiSeq Rapid SBS Kit v2 (50 cycles), Price: 587 CHF <br />
<br><br />
Question: <br />
* Are the "HiSeq Rapid Cluster Kit v2" compatible with HiSeq (before the v4 update). Or are the "TrueSeq Rapid Cluster Kits"?<br><br />
* What Kit can be clustered on our HiSeq?<br />
<br />
=== Software ===<br />
Our machine with SN# 700792 can not be updated to HiSeq Control Software (HCS) v2.2.37 or higher.<br><br />
Possible options are (depends on software on the FGPA, how to find out?):<br><br />
* HCS v1.5, RTA v1.13<br />
* HCS v2.0, RTA v1.17<br />
* HCS v2.2.38, RTA v1.18 - Not compatible<br />
* HCS v2.2.58, RTA v1.18 - Not compatible<br />
<br><br />
On the "do not eject" virtual drive from the machine it looks as if the last update was:<br><br />
override_2013-08-21__05_11_55.cfg<br />
<br><br />
So that was just after releas of HCS 2.0<br><br><br />
The HiSeq instrument computer employs 64-bit Windows Vista.<br><br />
<br><br />
Question: <br />
* Does V2.0 have a Rapid Run mode?<br />
* What is the "Include_Override.cfg" file?<br><br />
<br><br />
Download for hcs_1-5-15-1:<br><br />
https://support.illumina.com/downloads/hcs_1-5-15-1_rta_1-13_sav_1-8_software.html<br />
<br><br />
Download for hcs_2_0_12:<br><br />
https://support.illumina.com/downloads/hcs_2_0_12.html<br />
<br />
=== Workstation (Computer) ===<br />
<br />
Dell Precision T7500 Tower-Workstation <br><br />
AMD FirePro V3750 256MB<br><br />
48 Giga Ram<br><br />
<br><br />
[[File:29746587_1362014643899618_773048584_o.jpg|300px]]<br />
<br><br />
http://euro.dell.com/content/products/productdetails.aspx/workstation-precision-t7500?c=de&l=de&s=corp<br />
http://www.dell.com/support/article/us/en/4/sln291329/precision-t7500-windows-xp-and-windows-vista-driver-install-guide?lang=en#Broadcom57XXGigabitControllerDriver<br />
<br><br />
All the drives were removed from the computer when we got it. There is a raid controller board, an ethernet card and the Phoenix Video Card installed in the machine.<br><br />
<br />
[[File:29829408_1362014100566339_2138466687_o.jpg|400px]]<br><br />
<br><br />
Unfortunately the computer has a problem with the power supply and does not start up properly and we need to press the button on the powersupply to get it going somehow.<br><br />
<br><br />
[[File:GraficsCardHiSeq.jpg|300px]]<br />
The Phoenix PCI Express x1<br />
<br><br />
The screen comes with a touch screeen interface.<br><br />
<br />
<br />
=== Software Installation ===<br />
<br />
We had to completely reinstall the control computer. All the files needed to get the machine up and running could be found (2018) as free downloads on the internet. Here is how:<br><br />
<br><br />
* First install Windows Vista<br />
* Install the ethernet driver, the grafics card driver and the Chip-Set Driver from the Dell Support Hompage (links below)<br />
* Install the HiSeq Controll software (in our case we guessed Version HCS 2.0, depends on the FPGA version and SN of the machine) using the install.bat<br />
* The connect the HiSeq and turn it on, wait for 2 minutes until all the serial device show up<br />
* Then had to install the FTDI2xx64 driver for the USB serial ports from the FTDI website (for Windows Vista)<br />
* Also had to put a copy of the FTDI2XX.dll in the Illumina/ControlSoftware folder. (why?)<br />
* Install the Camera driver, reboot <br />
* Also put a copy of the camera.dll into the Illumina/ControlSoftware folder (not sure if needed).<br />
* Then the software keeps asking for an "Override" file on C:, so I copied to "Override_xx" file from the "DONOTEJECT" drive of the machine to C: and renamed it<br />
* Also the software needs drives up to E: for data / setting storage (just used some USB sticks)<br />
* Then start the control software and wait for the initialization to complete.<br />
<br />
<br />
http://www.dell.com/support/article/us/en/4/sln291329/precision-t7500-windows-xp-and-windows-vista-driver-install-guide?lang=en#Broadcom57XXGigabitControllerDriver<br />
<br />
<gallery><br />
File:29541446_1360455330722216_158399932171142298_n.jpg<br />
File:29542585_1360455254055557_5356302812154904650_n.jpg<br />
File:29570983_1360455394055543_5951203981908120697_n.jpg<br />
File:29572516_1360455187388897_4931162844279109332_n.jpg<br />
File:29572567_1360455437388872_3423434662908992089_n.jpg<br />
File:29573119_1360455220722227_2360941502472939739_n.jpg<br />
File:29597333_1361478547286561_4608098890389611846_n.jpg<br />
File:29597527_1360455147388901_3267016725697913962_n.jpg<br />
File:29683596_1360455294055553_8904862909943133518_n.jpg<br />
File:29693733_1362015353899547_577341277_o.jpg<br />
File:29693837_1362016817232734_2088113187_o.jpg<br />
File:29746366_1362016120566137_1611193266_o.jpg<br />
File:29747293_1362016660566083_714318785_o.jpg<br />
File:29829812_1362016310566118_1468621227_o.jpg<br />
File:29894022_1362016517232764_970806130_o.jpg<br />
</gallery><br />
<br />
After many tries the successfully initialized machine interface:<br />
<br />
[[File:29541640_1361478403953242_2086585179088537567_n.jpg|300px]]<br />
<br><br />
[[File:29683271_1361478447286571_3161554923032478769_n.jpg|300px]]<br />
<br><br />
Ready for some sequencing (or further tests)<br><br />
<br />
DCAP-api Driver:<br><br />
https://dcam-api.com/downloads/<br />
<br><br />
USB / Serial Port Sniffer that can be useful to reverse engineer the communication:<br><br />
https://freeusbanalyzer.com/<br />
<br><br />
https://docs.microsoft.com/en-us/sysinternals/downloads/portmon<br />
<br />
=== Links and Information ===<br />
<br />
Illumina Next Level Sequencing:<br><br />
https://www.youtube.com/watch?v=womKfikWlxM&feature=youtu.be<br />
<br><br />
Illumina Paired End Sequencing for Dummies:<br><br />
https://kscbioinformatics.wordpress.com/2017/02/13/illumina-sequencing-for-dummies-samples-are-sequenced/<br />
<br><br />
Expert Videos:<br><br />
https://www.youtube.com/playlist?list=PLKRu7cmBQlai-GUWeAN-eHD5xRcCXDW-D<br />
<br><br />
HiSeq2000 support page:<br><br />
https://support.illumina.com/sequencing/sequencing_instruments/hiseq_2000.html<br />
<br><br />
HiSeq 2000 User Guide:<br><br />
http://fantom.gsc.riken.jp/5/sstar/images/1/11/HiSeq2000_UserGuide_15011190_D.pdf<br><br />
<br><br />
<br />
HiSeq Compatibility Chart:<br><br />
https://support.illumina.com/content/dam/illumina-support/help/version_compatibility/Default.htm<br><br />
<br><br />
Video of the scanning:<br><br />
https://www.youtube.com/watch?v=tuD-ST5B3QA<br />
<br><br />
<br />
Illumina Support:<br><br />
Switzerland +41 565800000 +41 800200442<br />
<br />
=== Discussion ===<br />
The goal is to make it work!<br><br />
Let's discuss on the forum.. http://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/1 <br><br />
<br><br />
- Where to get/buy the reagents and flow cells<br><br />
- Hackquarium Lausanne got a similar machine and dissasembled it. We can get pointers from Gustavo on how to take it all apart!<br><br />
- Muffatto there's a fluorescence microscope inside (afaik), the issue is to reduce it in size and still have it working <br><br />
- Muffatto: erik from biocurious reverse engineered the chemistry of the system for BGI<br />
- Bengt: Absolutely in on building an openseq kind of thing that can run original reagents. And then re-engineering the system for smaller/cheaper/simpler - even better if combined with an effort to make open reagents - but that is 2 tracks that can progress independently <br><br />
<br><br />
<br />
=== Technical Descriptions / Findings ===<br />
====The fluorescent readout system with lasers and CCD cameras====<br />
<br><br />
[[File:HiSeq+Optics.jpg|650px]][[File:836px-FluorescenceFilters_2008-09-28.svg.png|300px]]<br><br />
Patent by Illumina:<br><br />
https://www.google.com/patents/DE202011003570U1?cl=it<br />
<br><br><br />
The HiSeq uses an epifluorescence microscope design shown in the diagram. Light of the excitation wavelength is focused on the specimen through the objective lens and the fluorescence emitted by the specimen is focused back the detector by the same objective.<br><br><br />
Here you can see the two camera units with even the letters A G T C written on it.<br><br />
[[File:IMG 20180201 221528.jpg|400px]]<br />
<br><br><br />
The laser calibration sheets that came with the machine:<br><br />
<gallery><br />
File:27707185_1310798825687867_500128244_o.jpg<br />
File:27744554_1310798829021200_1132936837_o.jpg<br />
File:IMG 20180202 102732.jpg<br />
</gallery><br />
<br><br />
<br><br />
The readout system of the HiSeq uses Line Imaging:<br><br />
[[File:Imaging-methods-in-Illumina-instruments-1024x364.jpg|600px]]<br />
<br />
====The Flow-Cell====<br />
<br><br />
[[File:FlowCell.jpg|700px]]<br />
[[File:Flowcell.jpg|400px]]<br />
<br><br />
The recommended maximum cluster density is 750,000-850,000 clusters/mm² when using Illumina's v3 cluster generation and sequencing reagents in combination with HCS v1.4. That makes 866²-921² clusters or 1.08-1.12 um² in average per cluster.<br />
<br><br><br />
<br />
====Some rough calculations/estimations on what is going on in the machine====<br />
<br><br />
The DNA clusters are about 1 Micrometer in size. (or bigger for older machines/software, maybe 2-5 um)<br> <br />
The lines on the flow cells are about 1 mm large what means 1000 clusters. (Or lager, up to 1.7 mm)<br> <br />
The reading speed is about 1mm per second or 1000x1000 clusters per second. Or 1 Mega bases.<br> <br />
The lines are 6 cm long and there are 10 lines per flow cell.And two flow cells.<br><br />
The means 600 Mega Clusters (Bases) per flowcell per complet run. And that takes about 600 seconds or 10 minutes.<br><br />
Then flush the flow-cell to add the next base (SBS, sequencing by sythesis). Then start over again.<br><br />
Unitl the whole 150 bases long DNA sequences are read.<br><br />
This takes about 4 days... 10 Genoms.<br><br />
The cluster are red in 4 colors / letters at the same time through 2 lasers exciting 4 colors in fluorescence. <br><br />
By 4 CCD line cameras with Time delay and integration (TDI). Line cameras with 1 micrometer resolution, 1000 lines a second. Or 1 picture 1000x1000 per second...<br><br />
The chemistry cost some hundreds to some thousands... but for what it does its not so bad. And you get the chemistry kit with the flowcell. So all the magic and the rest is just some kind of state of the art open hardware 🙂<br><br />
<br><br />
====Hardware components====<br />
Nice description on what hardware components are used in the HiSeq on the following page (see comments):<br><br />
https://blogs.swarthmore.edu/Illumina+GAIIx+Teardown/?p=125<br />
<br><br />
* two lasers (Laserquantum ignis 660nm, gem 532nm with SMD6000 drivers) <br />
* filter revolvers, beam expanders (Linos 2-8x) followed by a barrel lenses for each wavelength, a combiner to join the two excitation wavelengths<br />
* piezo actuator for the Z-Stage ([https://www.physikinstrumente.de/de/produkte/piezokeramische-aktoren/linearaktoren/p-601-piezomove-linearaktor-mit-fuehrungen-202600/ Physik Instrumente P-601] with driver E-601 and E-801 Sensor module) <br />
* Nikon CFI Plan APO VC 20x Objective.<br />
* XY-table (Parker 803-4099, something like the XR400 series, driven by a ViX-250-IH driver module).<br />
* The “Docking Station” with the Flowcells are mounted on three Stepper adjustable points to align them with the focal plane of the line scanner.<br />
* The fluorescence signal is divided by a fixed filter set 4x<br />
* 4 CCD cameras with S10405 line CCDs from Hamamatsu (DIL 40 package). <br />
* Two Hamamatsu Camera Control Boards (Model C10000-509) are each controlling two of these line cameras. <br />
* Illumina board with: line CCDs -> 8 LTC2203 25Msps 16-Bit ADCs -> Altera Cyclone II FPGA. Spartan XC3S4000 FPGA and XC95288 CPLD (both Xilinx) <br />
* Data is collected by the [https://www.activesilicon.com/products/phoenix-cpci-camera-link-frame-grabber/ Phoenix AS-PHX-D48CL Frame grabber card] in the Computer<br />
<br />
====Port usage====<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! Device<br />
! Port<br />
! Settings<br />
|-<br />
| ARM9BoardSerialPort / ARM9 Chem<br />
| Port: COM3; ARM9CHEM ;CM00006<br />
| 115200 baud<br />
|-<br />
| ARM9BoardDiagSerialPort / ARM9 Diag<br />
| Port: COM6; ARM9DIAG<br />
| <br />
|-<br />
|-<br />
|ARM9PEPort (PCIO) Board <br />
|Port: COM4; PCIO (com_port_num) <br />
|115200 baud<br />
|-<br />
<br />
|-<br />
|FPGA P1 Command / command_com_port_num<br />
|Port: COM10; IL000004 <br />
|115200 baud<br />
|-<br />
|-<br />
|FPGA P2 Response / response_com_port_num<br />
|Port: COM11; IL000005<br />
|115200 baud<br />
|-<br />
|-<br />
|<br>FlowcellFluidics1<br />
|<br />
|-<br />
|FtdiViciValve1 / VICI A1:<br />
|Port: COM16; VICIA1 ;CM00004<br />
|19200 baud (9600?)<br />
|-<br />
|FtdiViciValve2 / VICI A2:<br />
|Port: COM20; VICIA2 ;CM00043<br />
|19200 baud<br />
|-<br />
|KloehnControllerPump / Kloehn A<br />
|Port: COM18; KLOEHNA ;CM00001<br />
|9600 baud<br />
|-<br />
|<br>FlowcellFluidics2<br />
|<br />
|<br />
|-<br />
|FtdiViciValve1 / VICI B1:<br />
|Port: COM17; VICIB1 ;CM00002<br />
|19200 baud<br />
|-<br />
|FtdiViciValve2 / VICI B2:<br />
|Port: COM21; VICIB2 ;CM00044<br />
|19200 baud<br />
|-<br />
|KloehnControllerPump / Kloehn B<br />
|Port: COM19; KLOEHNB ;CM00003<br />
|9600 baud<br />
|-<br />
|<br>Laser 1,Green532,Smd6000<br />
|<br>Port: COM12; IL000006<br />
|<br>9600 baud<br />
|-<br />
|Laser 2,Red660,Smd6000<br />
|Port: COM13; IL000007<br />
|9600 baud<br />
|-<br />
|<br>XMotor_MDrive_5mm (X-Stage)<br />
|<br>Port: COM7; IL000001<br />
|<br>9600 baud<br />
|-<br />
|YMotor_VixServoIH_10nm (Y-Stage) / Servo linear motor MT49420<br />
|Port: COM8; IL000002<br />
|9600 baud<br />
|-<br />
<br />
|-<br />
|Z-Stage<br />
|Port: COM9; IL000003<br />
|<br />
|-<br />
<br />
|-<br />
|<br>Barcode_Reader<br />
|<br>Port: FTE2V9ML ; COM5 (com_port_num = 4)<br />
|<br />
|-<br />
|PTC<br />
|Port: COM2 (com_port_num = 1)<br />
|9600 baud<br />
|-<br />
|rs232<br />
|Port: COM10 (offset 9)<br />
|<br />
<br />
|-<br />
|Test Port <br />
|Port: IL000010<br />
|<br />
|-<br />
<br />
<br />
|-<br />
|PC Camera Ctrl<br />
|Port: COM14; IL000008<br />
|<br />
|-<br />
<br />
|-<br />
|<br>Phoenix Cam 1? / AS-PHX-D48CL-PE1 (C10000-509)<br />
|<br>Port: COM22 <br />
|<br />
|-<br />
|-<br />
|Phoenix Cam 2? / AS-PHX-D48CL-PE1 (C10000-509)<br />
|Port: COM23<br />
|<br />
|-<br />
<br />
|-<br />
|Flash Drive<br />
|Port: COM15; IL000009<br />
|<br />
|-<br />
<br />
|}<br />
Scanner.ChemistryModule<br />
<br />
[[File:PortList.jpg|100px]][[File:SerialPorts.jpg|100px]]<br />
<br><br />
During the software install I got this rather interesting list on the Hardware Manager showing all the device names.<br><br />
After the installation of the USB serial port driver the names disappeared.<br><br />
<br />
Ports:<br />
* ARM9 Chem<br />
* ARM9 Diag<br />
* Flash Drive<br />
* FPGA P1 Command<br />
* FPGA P2 Response<br />
* Kloehn A<br />
* Kloehn B<br />
* Laser 1<br />
* Laser 2<br />
* Multimedia Video Controller ?<br />
* PC Camera Ctrl ?<br />
* PCIO Board<br />
* Test Port ? <br />
* VICI A1<br />
* VICI A2<br />
* VICI B1<br />
* VICI B2<br />
* X-Stage<br />
* Y-Stage<br />
* Z-Stage<br />
<br />
====Drives====<br />
Data (D:)<br />
Data (E:)<br />
DVD (F:)<br />
Removable (G:)<br />
DoNoEject (H:)<br />
<br />
==== Comands ====<br />
<br />
'''FPGA'''<br />
<br>Main controll unit with a Field Programmable Gate Array (FPGA).<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! Command<br />
! Receive<br />
! Parameter<br />
! Function<br />
|-<br />
<br />
|-<br />
| colspan="4" |'''FPGA Board'''<br />
|-<br />
<br />
<br />
|-<br />
| MISCRVC\n<br />
| MISCRVC 3.0.14<br />
|<br />
| Reads version number of a board C<br />
|-<br />
<br />
|-<br />
| MISCRV\n<br />
| MISCRV 3.10.3<br />
|<br />
| Reads version number of a board<br />
|-<br />
<br />
|-<br />
| RESET\n<br />
| @LOG The FPGA is now online. Enjoy! / RESET<br />
|<br />
| Reset And Cancel All Commmands, is about to reset FPGA<br />
|-<br />
<br />
<br />
|-<br />
| colspan="4" |'''LED Bar'''<br />
|-<br />
<br />
|-<br />
| LEDMODEs m\n<br />
| LEDMODEs<br />
| s=[1,2]<br>m=[0,2,3,4,7]<br />
| Big front LED-bar light mode. s= left and right section (for each flow cell)<br />
|-<br />
<br />
|-<br />
| LEDPULSRATE r\n<br />
| LEDPULSRATE<br />
| r=[1-255] (milliseconds)<br />
| Puls rate of LED-bar. Controls, the frame update rate for the pulsing animation.<br />
|-<br />
<br />
|-<br />
| LEDSWPRATE r\n<br />
| LEDSWPRATE<br />
| r=[1-255] (milliseconds)<br />
| Sweeping rate of LED-bar. Controls, the frame update rate for the sweeping animation.<br />
|-<br />
<br />
|-<br />
| LEDMODETMPST 0\n<br />
| LEDMODETMPST<br />
|<br />
| ???<br />
|-<br />
<br />
|-<br />
| colspan="4" | '''Emission Filters''' <br />
|-<br />
<br />
<br />
|-<br />
| EM2VL_DN p\n<br />
| EM2VL_DN<br />
| p=[0..100? / 10,20,40] (percent)<br />
| Emission Filter 2, Velocity Percent Down<br />
|-<br />
<br />
|-<br />
| EM2VL_UP p\n<br />
| EM2VL_UP<br />
| p=[0..100? / 10,20,40] (percent)<br />
| Emission Filter 2, Velocity Percent Up<br />
|-<br />
<br />
<br />
|-<br />
| EM2RDV_DN\n<br />
| EM2RDV_DN v<br />
| v = [0..10?] 10<br />
| Emission Filter 2, Read position?<br />
|-<br />
<br />
|-<br />
| EM2RDV_UP\n<br />
| EM2RDV_UP v<br />
| v = [0..10?] 10<br />
| Emission Filter 2, Read position?<br />
|-<br />
<br />
|-<br />
|EM2RD\n<br />
|EM2RD 0 1 0 0<br />
|a b c d =[0,1] / a= , b=inPathSensor , c=OutOfPathSensor , d= / position = IntoPath / OutOfPath / Unknown <br />
|<br />
|-<br />
<br />
|-<br />
|EM2O\n EM2I\n EM2RD\n<br />
|<br />
|<br />
| Move filters / sensors ?<br />
|-<br />
|-<br />
| colspan="4" | '''Excitation Filters'''<br />
|-<br />
<br />
|-<br />
| EXsHM2\n<br />
| EXsHM2<br />
| s=[1,2] filter path<br />
| Home excitation filter 1 or 2 to laser-safe blocked position.<br />
|-<br />
<br />
|-<br />
| EXsMV p\n<br />
| <br />
| s=[1,2] p=[-71,71] (OD0.4) / 270.0000 degrees / 71 Moved 'Reflective' to index 2 (OD2.5) 90 degrees<br />
| ??<br />
|-<br />
<br />
|-<br />
| EXsVL p\n<br />
| <br />
| s=[1,2] p=[131072]<br />
| ??<br />
|-<br />
<br />
|-<br />
| EXsCUR p\n<br />
| <br />
| s=[1,2] p=[35]<br />
| ??<br />
|-<br />
<br />
|-<br />
| colspan="4" | '''Tilt Motor Control'''<br />
|-<br />
<br />
|-<br />
| TnMOVETO p\n<br />
| <br />
| n = [1,2,3] (motor), p=18504 (steps, 656 steps/mm) / (3025 steps (4.610 mm)?<br />
| Tilt motor (n) closed-loop absolute encoder moves.<br />
|-<br />
<br />
<br />
|-<br />
|TnRD\n<br />
|TnRD q<br />
|n = [1,2,3] (motor) q=steps ?encoder und motor steps equal?<br />
|Read tilt motor encoder.<br />
|-<br />
<br />
<br />
|-<br />
| TnVL p\n<br />
| T1VL<br />
| n = [1,2,3] (motor) p=62500<br />
| Tilt motor (n) set velocity ?<br />
|-<br />
<br />
<br />
|-<br />
| TnCUR 35\n<br />
| TnCUR<br />
| n = [1,2,3] (motor) p=35 <br />
| Motor Current?<br />
|-<br />
<br />
<br />
|-<br />
| T1CR<br />
| T1CR<br />
| <br />
| <br />
|-<br />
<br />
<br />
|-<br />
| T1HM<br />
| T1HM / @TILTPOS3 p<br />
| n = [1,2,3] (motor) / p=steps<br />
| Tilt motor (n) homing.<br />
|-<br />
<br />
<br />
|-<br />
| colspan="4" | '''Doors'''<br />
|-<br />
<br />
<br />
|-<br />
| DOORSSTATE p\n<br />
| DOORSSTATE<br />
| p=[0,1]<br />
| Set door state ?<br />
|-<br />
<br />
<br />
|-<br />
| DOORSOPEN\n<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| colspan="4" | '''Laser Control'''<br />
|-<br />
<br />
|-<br />
| LASERPB p\n<br />
| LASERPB p<br />
| p=[0..255/500/or more] (DAC/mW?)<br />
| Set laser power.<br />
|-<br />
<br />
<br />
|-<br />
| SWLSRSHUT p\n<br />
| SWLSRSHUT<br />
| p=[0,1] Closing / Opening laser shutter<br />
| controls laser shutter<br />
|-<br />
<br />
<br />
|-<br />
| LSEN\n<br />
| LSEN<br />
| <br />
| Laser Safety Enable Reporting<br />
|-<br />
<br />
|-<br />
| colspan="4" | '''TDI Control (Time Delay Integration)'''<br />
|-<br />
<br />
<br />
|-<br />
|ZMV p<br />
|ZMV<br />
| p=[1311..64224]<br />
| Z-Move And Trigger. Move Z-motor with ramp. ZMV will trigger camera immediately and ramp is HW controlled<br />
|-<br />
<br />
<br />
|-<br />
|TDIYEWR p\n<br />
|TDIYEWR<br />
| ? 6000012 / 6000008<br />
| TDI<br />
|-<br />
<br />
|-<br />
|TDIYARM2 2816 1\n <br />
|TDIYARM2<br />
| <br />
| TDI Scan Setup, Arm FPGA, FPGA Arm TDI<br />
|-<br />
<br />
<br />
|-<br />
|TDICLINES\n<br />
|TDICLINES q<br />
| q=3619<br />
| Returns number of clearing lines sent before image lines sent for the TDIYARM2 command<br />
|-<br />
<br />
|-<br />
|TDIPULSES\n<br />
|TDIPULSES 1613<br />
|<br />
|FPGA sent 1612 triggers to the camera. Actual triggers sent?<br />
|-<br />
<br />
|-<br />
|TDIYERD\n<br />
|TDIYERD 9108548<br />
|<br />
|Before scan, Y encoder from FPGA = 9108548 counts (91.085 mm)<br />
|-<br />
<br />
|-<br />
|TDIYPOS 9101938\n<br />
|TDIYPOS<br />
|<br />
|TDI Scan Setup, Arm FPGA, Set Y starting position for the TDIYARM2 command<br />
|-<br />
<br />
|-<br />
| TDIBB 0\n<br />
| <br />
| <br />
| TDI Brunos BBs something<br />
|-<br />
|-<br />
| TDIMASK 24\n<br />
| <br />
| <br />
| The version that supports the variable mask size of Hamamatsu's new camera's model<br />
|-<br />
|-<br />
| TDINEW_ATS\n<br />
| <br />
| <br />
|-<br />
|-<br />
| TDIZ_PI_STAGE\n<br />
| <br />
| <br />
| Offaxis Camera Initialize<br />
|-<br />
<br />
|-<br />
| colspan="4" | '''Z-Axis'''<br />
|-<br />
<br />
|-<br />
| ZADCR\n<br />
| ZADCR 1266<br />
|<br />
| ADC Read<br />
|-<br />
|-<br />
| <br />
| <br />
| ADC noise = 10, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 1263, posNoise = 10, negNoise = 10<br />
|-<br />
|-<br />
| ZDACW 63438\n<br />
| ZDACW<br />
|<br />
| ADC Write<br />
|-<br />
|-<br />
| ZADCAE 1\n<br />
| <br />
| <br />
|-<br />
|-<br />
| ZYT 0 3\n<br />
| ZYT<br />
| <br />
|-<br />
|-<br />
| ZTRG 0\n<br />
| ZTRG<br />
|<br />
| trigger<br />
|-<br />
<br />
|-<br />
| ZSPEC -47 65491\n<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| ZSTEP 1288471\n<br />
| <br />
| <br />
|-<br />
|-<br />
| ZSEL 0\n<br />
| <br />
| <br />
|-<br />
|-<br />
| ZCLK 1\n<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| colspan="4" | '''Compensator'''<br />
|-<br />
<br />
<br />
|-<br />
| MIOSPIINIT\n<br />
| <br />
| <br />
| Initialize Compensator<br />
|-<br />
<br />
|-<br />
| MIOCOMPR\n<br />
| <br />
|<br />
| Compensator: Selecting Galvo-type compensator<br />
|-<br />
<br />
|-<br />
| MIOCOMPF\n<br />
| <br />
|<br />
| <br />
|-<br />
<br />
|-<br />
| MIOSEL 2\n<br />
| <br />
| <br />
|-<br />
|-<br />
| MIOGSTEPS a b c\n<br />
| <br />
| 1 1 0 / 30 1 0 /30 5000 0 / 30 5000 2<br />
| Excitation Filter 1: Homed to center of sensor in 1292.4745 ms / Compensator: Moved OutOfPath in 420 ms<br />
|-<br />
<br />
<br />
|-<br />
| MIOGVAL p\n<br />
| <br />
| p= 0 0 / 200 0 / 200 130<br />
|-<br />
<br />
|-<br />
| MIOMVAL 0 0\n<br />
| <br />
| <br />
|-<br />
<br />
<br />
<br />
|-<br />
| colspan="4" | '''Software Settings?'''<br />
|-<br />
|-<br />
| SW_BRUNO 1\n<br />
| <br />
| <br />
| Set Bruno (Machine Type) Software<br />
|-<br />
|-<br />
| SWBEADCOMPAT p\n<br />
| <br />
| p=[0,1] 0 = Multi-channel dither, 1 = Original single channel dither & original normalization<br />
| Software channel dither control?<br />
|-<br />
<br />
|-<br />
| SWCEILING p\n<br />
|<br />
| p=..65535<br />
| Set max intensity value used in dither metric<br />
|-<br />
|-<br />
| SWCVGAIN1500\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWCVGAIN2500\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWCVHTPX 350\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWCVLIM1 3\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWCVLIM2 7\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWCVOFST 250\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWCVPRT1 0\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWDITH_IGAIN 100\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWDITH_IHIST 4\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWDITH_SHIFT 20\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWDITH_SIZE 100\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWFTLSR p\n<br />
| SWFTLSR<br />
| p=[0,1] (Off, On)<br />
| Control focus tracking laser<br />
|-<br />
<br />
|-<br />
| SWLINECOUNT 1\n<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| SWTIME p\n<br />
| <br />
| p=[0,1]<br />
| Stores microsecond timestamp in 6th pixel of each row of image<br />
|-<br />
<br />
|-<br />
| SWVIX p\n<br />
| <br />
| p=[0,1] <br />
| SWVIX (enable/disable VIX power) VixMotor?<br />
|-<br />
<br />
|-<br />
| SWYZ_POS 1\n<br />
| <br />
| <br />
|-<br />
|-<br />
| SWZLAG p\n<br />
| <br />
| p= 10 /25<br />
| Z-Axis lag?<br />
|-<br />
<br />
<br />
|-<br />
| TRAHSEN 0\n<br />
| <br />
| <br />
|-<br />
<br />
|}<br />
<br />
'''ARM9BoardSerialPort (ARM9CHEM)'''<br />
<br><br />
Chemistry controller with an ARM9 chip (ARM is a group of older 32-bit RISC processors).<br />
{| class="wikitable" border="1"<br />
|-<br />
! Command<br />
! Receive<br />
! Parameter<br />
! Function<br />
|-<br />
<br />
|-<br />
| ?IDN\r<br />
| Illumina,Bruno Fluidics Controller,0,v2.0340:A1\r\n<br />
| <br />
| Get Controller Version<br />
|-<br />
<br />
<br />
|-<br />
| ?PRETMP\r<br />
| 9.007C<br />
| <br />
|-<br />
<br />
|-<br />
| ?VSTAT\r<br />
| 3:A1\r\n<br />
| <br />
|-<br />
<br />
|-<br />
| ?VSENSE:1\r<br />
| 4:A1\r\n<br />
| <br />
|-<br />
<br />
AllDeviceSensorMonitor<br />
DoorSensorMonitor<br />
FlowSensorMonitor<br />
LiquidLevelSensorMonitor<br />
TemperatureSensorMonitor<br />
VacuumSensorMonitor<br />
PowerSensorMonitor<br />
BubbleSensorMonitor<br />
SipperSensorMonitor<br />
SolenoidSensorMonitor<br />
<br />
<br />
|-<br />
| ?FCTEMP:s:t\r <br />
| 23.824C:A1\r\n<br />
| s=[0,1] (flow cell) t=temp (°C)<br />
| Get Flow Cell temperature<br />
|-<br />
<br />
|-<br />
| FCTEMP:s:t\r <br />
| A1\r\n<br />
| s=[0,1] (flow cell) t=temp (°C)<br />
| Set Flow Cell temperature<br />
|-<br />
<br />
|-<br />
| FCTEMP:s:[P, I, D, S, F]:p\r (Proportional=0.8, Integral=0.2, Derivative=0, Frw_StepSize=1.875, Frw_Threshold=6.0 <br />
| A1\r\n<br />
| s=[0,1] (flow cell) p=value <br />
| Set Flow Cell temperature controller parameters.<br />
|-<br />
<br />
|-<br />
| FCRTD:s:[B , M]:p\r (Offset, Slope)<br />
| A1\r\n<br />
| s=[0,1] (flow cell) p=value <br />
| Set flow cell temperature sensor parameters. <br>(Resistance Temperature Detectors (RTD))<br />
|-<br />
<br />
|-<br />
| ?RETEMP:s:t\r ??<br />
| ??\r\n<br />
| s=[0,1] (flow cell) t=temp (°C)<br />
| Get reagent chiller temperature.<br />
|-<br />
<br />
<br />
|-<br />
| RETEMP:s:t\r <br>(Proportional=0.8, Integral=0.2, Derivative=0, Feed-Frw_StepSize=1.875, Feed-Frw_Threshold=6.0)<br />
| A1\r\n<br />
| s=[0,1] (flow cell) t=temp (°C)<br />
| Set reagent chiller temperature.<br />
|-<br />
<br />
|-<br />
| RETEC:s:[P, I, D, S, F]:p\r <br>(Proportional=0.8, Integral=0.2, Derivative=0, Feed-Frw_StepSize=1.875, Feed-Frw_Threshold=6.0)<br />
| A1\r\n<br />
| s=[0,1] (reagent chiller) p=value <br />
| Set reagent chiller temperature controller parameters. <br>Thermoelectric cooler (TEC)<br />
|-<br />
<br />
|-<br />
| RERTD:s:[B , M]:p\r (Offset, Slope)<br />
| A1\r\n<br />
| s=[0,1] (reagent chiller) p=value <br />
| Set reagent chiller temperature sensor parameters. <br>(Resistance Temperature Detectors (RTD))<br />
|-<br />
<br />
|-<br />
|?PRETMP\r<br />
|10.937C:A1\r\n<br />
|<br />
|<br />
|-<br />
<br />
|-<br />
| ?asyphon:0\r<br />
|<br />
|p=[0,1] <br />
| Read syphone (pump)<br />
|-<br />
<br />
<br />
|-<br />
| asyphon:p:q\r<br />
|<br />
|p=[0,1] , p=[0,1]<br />
| Aspire from syphone (pump)<br />
|-<br />
<br />
<br />
<br />
|-<br />
| <br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| ?HDDRVER\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| ?IDN\r<br />
| <br />
| <br />
|-<br />
<br />
<br />
|-<br />
| VACUUM:p\r<br />
| A1\r\n<br />
| p=[0,1]<br />
| Turn vacuum on and off.<br />
|-<br />
<br />
<br />
|-<br />
| DAC:4:2130\r<br />
| <br />
| <br />
|-<br />
<br />
<br />
|-<br />
| <br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| INIT\r<br />
| <br />
| <br />
|-<br />
<br />
<br />
<br />
<br />
|-<br />
| STRMASK:2:2\r<br />
| <br />
| <br />
|-<br />
<br />
<br />
<br />
|}<br />
<br />
'''ARM9PEPort (PCIO)'''<br />
<br>Program Controlled Input/Output Board (PCIO) with an ARM9 chip.<br />
<br />
{| class="wikitable" border="1"<br />
<br />
|-<br />
! Command<br />
! Receive<br />
! Parameter<br />
! Function<br />
|-<br />
<br />
<br />
|-<br />
| ?IDN\r<br />
| Illumina,PCIO Controller,0,v0.0035:A1\r\n<br />
| <br />
| Get Controller Version<br />
|-<br />
<br />
|-<br />
|?HDDRVER\r<br />
|Recv 0.000000:A1\r\n<br />
|<br />
|<br />
|-<br />
<br />
<br />
|-<br />
| STRMASK:2:2\r<br />
| <br />
| <br />
|-<br />
<br />
<br />
|-<br />
| WASTE:0:1\r<br />
| A1\r\n<br />
|<br />
|p=[0,1] , p=[0,1]<br />
| Waste cotroll<br />
|-<br />
<br />
|}<br />
<br />
'''X-Motor'''<br />
{| class="wikitable" border="1"<br />
|-<br />
! Command<br />
! Receive<br />
! Parameter<br />
! Function<br />
|-<br />
<br />
<br />
|-<br />
| PR VI<br />
| PR VI\r\n410\r\n<br />
| 410 (1.0009765625 mm/sec)<br />
| Parameter Read velocity<br />
|-<br />
<br />
|-<br />
| PR VM<br />
| PR VM\r\n6144\r\n<br />
| 6144 (15 mm/sec)<br />
| Parameter Read velocity<br />
|-<br />
<br />
<br />
|-<br />
| VM=4096<br />
| VM=4096\r\n<br />
| <br />
| <br />
|-<br />
<br />
<br />
|-<br />
| VI=1<br />
| VI=1\r\n<br />
| <br />
| Setting velocity for finding negative limit sensor to 10.00 mm/sec<br />
|-<br />
<br />
<br />
|-<br />
| MR -409190<br />
| MR -409190\r\n<br />
| -409190 to negative limit<br />
| moving and read ?<br />
|-<br />
<br />
<br />
|-<br />
| Xmit<br />
| Recv<br />
| <br />
|-<br />
<br />
|-<br />
| <br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| DE=1\n<br />
| DE=1\r\n><br />
| <br />
|-<br />
<br />
|-<br />
| EX 1\n<br />
| <br />
| exit?<br />
|-<br />
<br />
|-<br />
| MA -4915\n<br />
| MA -4915\r\n><br />
| <br />
|-<br />
<br />
|-<br />
| MR -409190\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| <br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| PM=0\n<br />
| PM=0\r\n><br />
| Homing part 3 finished OK<br />
|-<br />
<br />
|-<br />
| <br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| PR ER\n<br />
| PR ER\r\n0\r\n><br />
| <br />
|-<br />
<br />
|-<br />
| PR ER\r<br />
| PR ER\r\n84\r\n><br />
| <br />
|-<br />
<br />
|-<br />
| PR MV\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| PR VI\n<br />
| PR VI\r\n410\r\n><br />
| <br />
|-<br />
<br />
|-<br />
| PR VM\n<br />
| PR VM\r\n6144\r\n><br />
| <br />
|-<br />
<br />
|-<br />
| <br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| VI=410\n<br />
| VI=410\r\n><br />
| After Checking for motion / negative limit sensor, response = 'PR MV\r\n1\r\n?'<br />
|-<br />
<br />
|-<br />
| VM=4096\n<br />
| VM=4096\r\n><br />
| <br />
|-<br />
<br />
<br />
<br />
|}<br />
<br />
<br />
<br />
'''Laser1'''<br />
{| class="wikitable" border="1"<br />
|-<br />
! Command<br />
! Receive<br />
! Parameter<br />
! Function<br />
|-<br />
<br />
|-<br />
| VERSION?\r<br />
| SMD-G-1.1.2\r\n\r\n<br />
| <br />
| Read Laser Version.<br />
|-<br />
<br />
|-<br />
| STAT?\r<br />
| ENABLED\r\n<br />
| <br />
| Read laser status.<br />
|-<br />
<br />
|-<br />
| POWER=p\r<br />
| <br />
| p=[0,430] (milliwatt)<br />
| Set laser power.<br />
|-<br />
<br />
<br />
|-<br />
| POWER?\r<br />
| pmW\r\n (0000mW\r\n)<br />
| p (milliwatt)<br />
| Read laser power.<br />
|-<br />
<br />
<br />
|-<br />
| ON\r<br />
| <br />
| <br />
| Turn laser on.<br />
|-<br />
<br />
|-<br />
| OFF\r<br />
| <br />
| <br />
| Turn laser off.<br />
|-<br />
<br />
|-<br />
| \n\n<br />
| <br />
| <br />
|-<br />
<br />
<br />
<br />
|}<br />
<br />
<br />
<br />
<br />
'''KLOEHNA'''<br />
{| class="wikitable" border="1"<br />
|-<br />
! Command<br />
! Parameter<br />
! Function<br />
|-<br />
<br />
|-<br />
| /1\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1&\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1IR\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1L7R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1l7R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1L7R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1l7R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1L7R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1l7R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1L7R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1l7R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1OR\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1V10000A0R\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| /1W4R\r<br />
| <br />
| <br />
|-<br />
<br />
<br />
<br />
|}<br />
<br />
<br />
<br />
'''VICIA1'''<br />
{| class="wikitable" border="1"<br />
|-<br />
! Command<br />
! Parameter<br />
! Function<br />
|-<br />
<br />
|-<br />
| CC9\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| CP\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| CW1\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| VR\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| CC1\r<br />
| <br />
| <br />
|-<br />
<br />
|-<br />
| CW2\r<br />
| <br />
| <br />
|-<br />
<br />
<br />
<br />
|}<br />
<br><br />
'''Thermal Sensor and Regulation Parameter'''<br />
<br><br />
Thermal calibration - flow cells (Calibrated during manufactoring for each instrument)<br><br />
FlowCell1RTD_Slope= 0.957142;0.92;0.9742857<br><br />
FlowCell1RTD_Offset= 0.8571434;1.800001;0.764286<br><br />
FlowCell2RTD_Slope= 0.951428;0.91;0.9471429<br><br />
FlowCell2RTD_Offset= 0.9714298;1.849998;1.207142<br><br />
<br><br />
Thermal calibration - chiller<br><br />
Reagent1RTD_Slope= 1 <br><br />
Reagent1RTD_Offset= 0<br><br />
Reagent2RTD_Slope= 1<br><br />
Reagent2RTD_Offset= 0<br><br />
Reagent3RTD_Slope= 0.9833334<br><br />
Reagent3RTD_Offset= 0.2833328<br><br />
<br><br />
Temperature servo-loop parameters (Instrument type dependent, e.g., Bruno vs. Harmonia)<br><br />
FlowCell1TEC_Servo_Proportional= 0.2 <br><br />
FlowCell1TEC_Servo_Integral= 0.1<br><br />
FlowCell1TEC_Servo_Derivative= 0.0<br><br />
FlowCell1TEC_Feed_Frw_StepSize= 1.875<br><br />
FlowCell1TEC_Feed_Frw_Threshold= 6.0 <br><br />
FlowCell2TEC_Servo_Proportional= 0.2 <br> <br />
FlowCell2TEC_Servo_Integral= 0.1<br><br />
FlowCell2TEC_Servo_Derivative= 0.0<br><br />
FlowCell2TEC_Feed_Frw_StepSize= 1.875<br><br />
FlowCell2TEC_Feed_Frw_Threshold= 6.0<br><br />
<br><br />
Chiller servo-loop parameters <br><br />
Reagent1TEC_Servo_Proportional= 0.8 <br><br />
Reagent1TEC_Servo_Integral= 0.2<br><br />
Reagent1TEC_Servo_Derivative= 0.0<br><br />
Reagent1TEC_Feed_Frw_StepSize= 1.875<br><br />
Reagent1TEC_Feed_Frw_Threshold= 6.0 <br><br />
Reagent2TEC_Servo_Proportional= 0.8<br><br />
Reagent2TEC_Servo_Integral= 0.2<br><br />
Reagent2TEC_Servo_Derivative= 0.0<br><br />
Reagent2TEC_Feed_Frw_StepSize= 1.875<br><br />
Reagent2TEC_Feed_Frw_Threshold= 6.0 <br><br />
Reagent3TEC_Servo_Proportional= 1.7<br><br />
Reagent3TEC_Servo_Integral= 1.1<br><br />
Reagent3TEC_Servo_Derivative= 0.0<br><br />
<br><br />
<br><br />
[ExcitationFilter1]<br><br />
FPGACommandSuffix = 1<br><br />
0 = BlockPosition, Home ; NOTE!!! If spelling is changed, C# code must also be changed!!! <br><br />
1 = OD4.0<br><br />
2 = OD2.0<br><br />
3 = OD0.6<br><br />
4 = OpenBandPassPosition ; Open. NOTE!!! If spelling is changed, C# code must also be changed!!! <br><br />
5 = OD0.2<br><br />
6 = OD1.4<br><br />
7 = OD1.5<br><br />
<br><br />
[ExcitationFilter2]<br><br />
FPGACommandSuffix = 2<br><br />
0 = BlockPosition,Home ; NOTE!!! If spelling is changed, C# code must also be changed!!!<br> <br />
1 = OD4.5<br><br />
2 = OD3.0<br><br />
3 = OD2.0<br><br />
4 = OpenBandPassPosition ; Open. NOTE!!! If spelling is changed, C# code must also be changed!!! <br><br />
5 = OD0.2<br><br />
6 = OD0.9<br><br />
7 = OD1.0<br><br />
<br><br />
[ChromaticCompensator]<br><br />
FPGACommandSuffix = 3<br><br />
Positions = 0, 399, 799, 1199 <br><br />
0 = Home, Open, Sequencing_AC<br><br />
1 = Unused1, Sequencing_GT<br><br />
2 = Unused2<br><br />
3 = Unused3, Beadchip<br><br />
<br><br />
; Each filter set represents 4 positions on a wheel<br><br />
[EmissionFilterSet1] ; This is for SensorPath 1<br><br />
FPGACommandSuffix = 1<br><br />
NumPositions = 4<br><br />
Positions = 0, 399, 799, 1199<br><br />
0 = Filter.610-60, Home, Blocked <br> <br />
1 = Filter.740-60 <br> <br />
2 = Wedge <br> <br />
3 = Open<br><br />
<br><br />
[EmissionFilterSet2]<br><br />
FPGACommandSuffix = 2<br><br />
NumPositions = 4<br><br />
Positions = 0, 399, 799, 1199<br><br />
0 = Filter.687-20, Home, Blocked <br> <br />
1 = Filter.558-32 <br> <br />
2 = Wedge <br> <br />
3 = Open<br><br />
<br><br />
VacuumDACSetting= 2500 ; 0 to 4095, HiSeq is 2130<br><br />
<br />
=== Optical Path ===<br />
<br><br />
[Analytical Channels]<br><br />
Channel1 = Grn<br><br />
Channel2 = Red<br><br />
<br><br />
[Sequencing Channels]<br><br />
Channel1 = C<br><br />
Channel2 = A<br><br />
Channel3 = T<br><br />
Channel4 = G<br><br />
<br><br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! Path<br />
! SensorPath<br />
! LaserPath<br />
! ExcitationPosition<br />
! EmissionPosition<br />
! ChromaticCompensatorPosition<br />
|-<br />
<br />
|-<br />
| Analytical Channel Grn<br />
| 1<br />
| 1<br />
| OpenBandPassPosition<br />
| Filter.610-60<br />
| Beadchip<br />
|-<br />
<br />
|-<br />
| Analytical Channel Red<br />
| 2<br />
| 2<br />
| OpenBandPassPosition<br />
| Filter.687-20<br />
| Beadchip<br />
|-<br />
<br />
<br />
|-<br />
| Analytical Channel Reflective<br />
| 2<br />
| 2<br />
| OD2.5 Only Ex 2<br />
| Open <br />
| Open<br />
|-<br />
<br />
<br />
|-<br />
| Analytical Channel Reflective Autocenter<br />
| 2<br />
| 2<br />
| OD2.5 Only Ex 2<br />
| Open <br />
| Open<br />
|-<br />
<br />
<br />
|-<br />
| Analytical Channel Emissive<br />
| 1<br />
| 1<br />
| OD1.0 ETF like scanning<br />
| Filter.610-60 <br />
| Beadchip<br />
|-<br />
<br />
<br />
|-<br />
| Sequencing Channel A<br />
| 2<br />
| 2<br />
| OpenBandPassPosition<br />
| Filter.687-20<br />
| Sequencing_AC<br />
|-<br />
<br />
<br />
|-<br />
| Sequencing Channel T<br />
| 1<br />
| 1<br />
| OpenBandPassPosition<br />
| Filter.610-60<br />
| Sequencing_GT<br />
|-<br />
<br />
<br />
|-<br />
| Sequencing Channel G<br />
| 2<br />
| 1<br />
| OpenBandPassPosition<br />
| Filter.558-32<br />
| Sequencing_GT<br />
|-<br />
<br />
|-<br />
| Sequencing Channel C<br />
| 1<br />
| 2<br />
| OpenBandPassPosition<br />
| Filter.740-60<br />
| Sequencing_AC<br />
|-<br />
<br />
|-<br />
| Sequencing Channel Reflective<br />
| 2<br />
| 2<br />
| OD2.5 Only Ex2<br />
| Open<br />
| Open<br />
|-<br />
<br />
|-<br />
| Sequencing Channel Emissive<br />
| 1<br />
| 2<br />
| OpenBandPassPosition<br />
| Filter.740-60<br />
| Open<br />
|-<br />
<br />
|}<br />
<br />
=== OpenSeq ===<br />
How about making an Open Source Next Level Sequencing machine - OpenSeq.<br><br />
Maybe a bit slower and smaller.. like 1 genome per day 🙂<br><br />
Not sure a price tag of 500k euro is justified for such a machine...<br><br />
Maybe similar to the iSeq100:<br><br />
https://www.illumina.com/systems/sequencing-platforms/iseq.html</div>Derishushttp://www.hackteria.org/wiki/index.php?title=HiSeq2000_-_Next_Level_Hacking&diff=27947HiSeq2000 - Next Level Hacking2018-03-28T22:47:24Z<p>Derishus: </p>
<hr />
<div>[[File:HiSeq.jpg|400px]]<br />
<br />
We got a HiSeq 2000, Next Level Sequencing Machine from the Genomics Facility of Department of Biosystems Science and Engineering in Basel. Contact through Biozentrum, University of Basel. We got it for free with the only disclaimer: "The biohackers should understand that they are responsible to organize and pay for the transport as well as that there is no warranty or support that can be given neither by us nor the DBSSE." <br><br />
<br><br />
This type of machine seems to be quite difficult to get up and running and also reagents, flowcell-kits and software licences can be expensive. Since more of these machines seem to show up in second hand (there are new machine generations by Illumina) it would be worth trying to find a way to make them work. Sequencing for all.<br><br />
<br><br />
Specifications:<br><br />
https://www.illumina.com/documents/products/datasheets/datasheet_hiseq2000.pdf<br />
<br><br />
The HiSeq2000 (200Gb) was introduced in the year 2010. Followed by HiSeq2500 (500Gb) in 2012. And HiSeq X Ten (1000Gb) in 2014. In 2017 the NovaSeq series of machines was launched.<br><br />
The machine is a quite early on, from March 2011, Serial Number is 700792, so the machine can not be updated to software and chemistry v4. Only machines with SN# <br />
7001403 or higher can get the FPGA [https://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/upgrades/hiseq_v4_upgrade_information_sheet_140328_3.pdf update v4].<br><br />
[[File:27747171_1313614108739672_1344364391_o.jpg|100px]]<br />
<br><br />
<br><br />
=== First Inspection ===<br />
I made a first inspection on the machine. It seems very well made (2011). I still think it would be cool to make it run as is. It's basically a big microfluidic system. So if we get the pumps and the cameras to work we can hack it into anything we want 🙂. Even if it's not for sequencing - it's basically a holder for flow-cells with a fluorescence camera attached to it. And 32 channels with pumps and selector valves that attach to the flow cells. Plus a fridge and a computer.<br />
And peltier for heating and cooling (pcr). Now trying to get the control software. I also think the system is "relatively" open... the software can be downloaded and kind of installs, there is no ID checking on the supplies or anything. Looks very hackable. Also all the cases can be opened easily. Let's do a weekend hack-session on it.<br><br />
<br><br />
I think it's great opportunity to learn about next level sequencing and about how theses machines work.<br><br />
<br><br />
Fluidic System:<br><br />
[[File:27356260_1310155019085581_6425750036040119481_o.jpg|600px]]<br />
<br><br><br />
Some pictures from the inside of the machine:<br><br />
<gallery><br />
File:IMG_20180201_155204.jpg<br />
File:IMG_20180201_155208.jpg<br />
File:IMG_20180201_155416.jpg<br />
File:IMG_20180201_211136.jpg<br />
File:IMG_20180201_211144.jpg<br />
File:IMG_20180201_211925.jpg<br />
File:IMG_20180201_212240.jpg<br />
File:IMG_20180201_212251.jpg<br />
File:IMG_20180201_212316.jpg<br />
File:IMG_20180201_212444.jpg<br />
File:IMG_20180201_212453.jpg<br />
File:IMG_20180201_213250.jpg<br />
File:IMG_20180201_213258.jpg<br />
File:IMG_20180201_214433.jpg<br />
File:IMG_20180201_221007.jpg<br />
File:IMG_20180201_221216.jpg<br />
File:IMG_20180201_221229.jpg<br />
File:IMG_20180201_221420.jpg<br />
File:IMG_20180201_221434.jpg<br />
File:IMG_20180201_221528.jpg<br />
File:IMG_20180201_222654.jpg<br />
File:IMG_20180201_223556.jpg<br />
File:IMG_20180202_085730.jpg<br />
File:IMG_20180202_102732.jpg<br />
</gallery><br />
Lausanne Bio-Hackerspace Hackuarium got a HiSeq2000 (SN# 700918) and Gustavo dissected it. Here some pictures that Rachel sent me with comments form what I think components are:<br />
<br />
[[File:HiSeqDisection.jpg|300px]][[File:HiSeqDisection2.jpg|300px]]<br><br><br />
<br />
=== Chemistry ===<br />
Some images describing the Illumina Next-Generation Sequencing Chemistry:<br><br />
[[File:Chemistry.png|400px]]<br />
<gallery><br />
File:figure1.png<br />
File:ilmn-step7-12.jpg<br />
File:reversible-terminator-sequencing-large.png<br />
</gallery><br />
[[File:HiSeqProcess.jpg|480px]]<br><br />
Illumina uses a process called "Sequencing-by-synthesis"<br><br />
The HiSeq (and MiSeq) use 4-colour SBS<br><br />
<br><br><br />
The full DNA to Data solution:<br><br />
[[File:Process.png|600px]]<br />
<br><br><br />
Paper on the 4 color SBS:<br><br />
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1702316/<br />
<br><br><br />
Video with the possibilities for library preparation:<br><br />
https://www.youtube.com/watch?v=_yC0Bzw3WbQ<br><br />
Library prep kit with sample purification by magnetic beads:<br><br />
https://www.youtube.com/watch?v=UE1TAZZPUFI<br />
<br><br><br />
The CBot 2 System is used to prepare the clusters on the flow cell:<br><br />
https://emea.illumina.com/products/by-type/accessory-products/cbot.html<br />
<br><br><br />
HiSeq (and TrueSeq) Rapid Cluster Kits can be clustered on the HiSeq (on-board cluster). They also seem to have a lower read and are cheaper:<br><br />
https://emea.illumina.com/products/by-type/sequencing-kits/cluster-gen-sequencing-reagents/hiseq-rapid-cluster-kit-pe-sr.html<br />
<br><br><br />
===== Chemisty Kits Option (running on our machine): =====<br />
<gallery><br />
File:hiseq-rapid-cluster-kit-pe-sr.jpg<br />
File:hiseq-rapid-sbs-kit.jpg<br />
</gallery><br />
<br />
All Library Prep Kits<br><br />
Cluster Generation (cBot needed):<br />
* TruSeq SR Cluster Kit v3 - cBot-HS - Price: 4360 CHF<br />
* TruSeq PE Cluster Kit v3 - cBot-HS - Price: 6674 CHF<br />
* TruSeq Rapid SR and PE Cluster Kits, cBot Duo Cluster Kit - Price: Request<br />
Maybe not compatible (for reference):<br><br />
* HiSeq SR Rapid Cluster Kit v2, Price: 1002 CHF<br />
* HiSeq PE Rapid Cluster Kit v2, Price: 1540 CHF<br />
<br />
SBS Reagents:<br />
* TruSeq SBS Kit v3 - HS (50 cycles), Price: 2552 CHF<br />
* TruSeq Rapid SBS Kit - HS, Price: Request<br />
Maybe not compatible (for reference):<br><br />
*HiSeq Rapid SBS Kit v2 (50 cycles), Price: 587 CHF <br />
<br><br />
Question: <br />
* Are the "HiSeq Rapid Cluster Kit v2" compatible with HiSeq (before the v4 update). Or are the "TrueSeq Rapid Cluster Kits"?<br><br />
* What Kit can be clustered on our HiSeq?<br />
<br />
=== Software ===<br />
Our machine with SN# 700792 can not be updated to HiSeq Control Software (HCS) v2.2.37 or higher.<br><br />
Possible options are (depends on software on the FGPA, how to find out?):<br><br />
* HCS v1.5, RTA v1.13<br />
* HCS v2.0, RTA v1.17<br />
* HCS v2.2.38, RTA v1.18 - Not compatible<br />
* HCS v2.2.58, RTA v1.18 - Not compatible<br />
<br><br />
On the "do not eject" virtual drive from the machine it looks as if the last update was:<br><br />
override_2013-08-21__05_11_55.cfg<br />
<br><br />
So that was just after releas of HCS 2.0<br><br><br />
The HiSeq instrument computer employs 64-bit Windows Vista.<br><br />
<br><br />
Question: <br />
* Does V2.0 have a Rapid Run mode?<br />
* What is the "Include_Override.cfg" file?<br><br />
<br><br />
Download for hcs_1-5-15-1:<br><br />
https://support.illumina.com/downloads/hcs_1-5-15-1_rta_1-13_sav_1-8_software.html<br />
<br><br />
Download for hcs_2_0_12:<br><br />
https://support.illumina.com/downloads/hcs_2_0_12.html<br />
<br />
=== Workstation (Computer) ===<br />
<br />
Dell Precision T7500 Tower-Workstation <br><br />
AMD FirePro V3750 256MB<br><br />
48 Giga Ram<br><br />
<br><br />
<br />
http://euro.dell.com/content/products/productdetails.aspx/workstation-precision-t7500?c=de&l=de&s=corp<br />
http://www.dell.com/support/article/us/en/4/sln291329/precision-t7500-windows-xp-and-windows-vista-driver-install-guide?lang=en#Broadcom57XXGigabitControllerDriver<br />
<br />
=== Links and Information ===<br />
<br />
Illumina Next Level Sequencing:<br><br />
https://www.youtube.com/watch?v=womKfikWlxM&feature=youtu.be<br />
<br><br />
Expert Videos:<br><br />
https://www.youtube.com/playlist?list=PLKRu7cmBQlai-GUWeAN-eHD5xRcCXDW-D<br />
<br><br />
HiSeq2000 support page:<br><br />
https://support.illumina.com/sequencing/sequencing_instruments/hiseq_2000.html<br />
<br><br />
HiSeq 2000 User Guide:<br><br />
http://fantom.gsc.riken.jp/5/sstar/images/1/11/HiSeq2000_UserGuide_15011190_D.pdf<br><br />
<br><br />
<br />
HiSeq Compatibility Chart:<br><br />
https://support.illumina.com/content/dam/illumina-support/help/version_compatibility/Default.htm<br><br />
<br><br />
Video of the scanning:<br><br />
https://www.youtube.com/watch?v=tuD-ST5B3QA<br />
<br><br />
<br />
Illumina Support:<br><br />
Switzerland +41 565800000 +41 800200442<br />
<br />
=== Discussion ===<br />
The goal is to make it work!<br><br />
Let's discuss on the forum.. http://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/1 <br><br />
<br><br />
- Where to get/buy the reagents and flow cells<br><br />
- Hackquarium Lausanne got a similar machine and dissasembled it. We can get pointers from Gustavo on how to take it all apart!<br><br />
- Muffatto there's a fluorescence microscope inside (afaik), the issue is to reduce it in size and still have it working <br><br />
- Muffatto: erik from biocurious reverse engineered the chemistry of the system for BGI<br />
- Bengt: Absolutely in on building an openseq kind of thing that can run original reagents. And then re-engineering the system for smaller/cheaper/simpler - even better if combined with an effort to make open reagents - but that is 2 tracks that can progress independently <br><br />
<br><br />
<br />
=== Technical Descriptions / Findings ===<br />
====The fluorescent readout system with lasers and CCD cameras====<br />
<br><br />
[[File:HiSeq+Optics.jpg|650px]][[File:836px-FluorescenceFilters_2008-09-28.svg.png|300px]]<br><br />
Patent by Illumina:<br><br />
https://www.google.com/patents/DE202011003570U1?cl=it<br />
<br><br><br />
The HiSeq uses an epifluorescence microscope design shown in the diagram. Light of the excitation wavelength is focused on the specimen through the objective lens and the fluorescence emitted by the specimen is focused back the detector by the same objective.<br><br><br />
Here you can see the two camera units with even the letters A G T C written on it.<br><br />
[[File:IMG 20180201 221528.jpg|400px]]<br />
<br><br><br />
The laser calibration sheets that came with the machine:<br><br />
<gallery><br />
File:27707185_1310798825687867_500128244_o.jpg<br />
File:27744554_1310798829021200_1132936837_o.jpg<br />
File:IMG 20180202 102732.jpg<br />
</gallery><br />
<br><br />
<br><br />
The readout system of the HiSeq uses Line Imaging:<br><br />
[[File:Imaging-methods-in-Illumina-instruments-1024x364.jpg|600px]]<br />
<br />
====The Flow-Cell====<br />
<br><br />
[[File:FlowCell.jpg|700px]]<br />
[[File:Flowcell.jpg|400px]]<br />
<br><br />
The recommended maximum cluster density is 750,000-850,000 clusters/mm² when using Illumina's v3 cluster generation and sequencing reagents in combination with HCS v1.4. That makes 866²-921² clusters or 1.08-1.12 um² in average per cluster.<br />
<br><br><br />
<br />
====Some rough calculations/estimations on what is going on in the machine====<br />
<br><br />
The DNA clusters are about 1 Micrometer in size. (or bigger for older machines/software, maybe 2-5 um)<br> <br />
The lines on the flow cells are about 1 mm large what means 1000 clusters. (Or lager, up to 1.7 mm)<br> <br />
The reading speed is about 1mm per second or 1000x1000 clusters per second. Or 1 Mega bases.<br> <br />
The lines are 6 cm long and there are 10 lines per flow cell.And two flow cells.<br><br />
The means 600 Mega Clusters (Bases) per flowcell per complet run. And that takes about 600 seconds or 10 minutes.<br><br />
Then flush the flow-cell to add the next base (SBS, sequencing by sythesis). Then start over again.<br><br />
Unitl the whole 150 bases long DNA sequences are read.<br><br />
This takes about 4 days... 10 Genoms.<br><br />
The cluster are red in 4 colors / letters at the same time through 2 lasers exciting 4 colors in fluorescence. <br><br />
By 4 CCD line cameras with Time delay and integration (TDI). Line cameras with 1 micrometer resolution, 1000 lines a second. Or 1 picture 1000x1000 per second...<br><br />
The chemistry cost some hundreds to some thousands... but for what it does its not so bad. And you get the chemistry kit with the flowcell. So all the magic and the rest is just some kind of state of the art open hardware 🙂<br><br />
<br><br />
====Hardware components====<br />
Nice description on what hardware components are used in the HiSeq on the following page (see comments):<br><br />
https://blogs.swarthmore.edu/Illumina+GAIIx+Teardown/?p=125<br />
<br><br />
* two lasers (Laserquantum ignis 660nm, gem 532nm with SMD6000 drivers) <br />
* filter revolvers, beam expanders (Linos 2-8x) followed by a barrel lenses for each wavelength, a combiner to join the two excitation wavelengths<br />
* piezo actuator for the Z-Stage ([https://www.physikinstrumente.de/de/produkte/piezokeramische-aktoren/linearaktoren/p-601-piezomove-linearaktor-mit-fuehrungen-202600/ Physik Instrumente P-601] with driver E-601 and E-801 Sensor module) <br />
* Nikon CFI Plan APO VC 20x Objective.<br />
* XY-table (Parker 803-4099, something like the XR400 series, driven by a ViX-250-IH driver module).<br />
* The “Docking Station” with the Flowcells are mounted on three Stepper adjustable points to align them with the focal plane of the line scanner.<br />
* The fluorescence signal is divided by a fixed filter set 4x<br />
* 4 CCD cameras with S10405 line CCDs from Hamamatsu (DIL 40 package). <br />
* Two Hamamatsu Camera Control Boards (Model C10000-509) are each controlling two of these line cameras. <br />
* Illumina board with: line CCDs -> 8 LTC2203 25Msps 16-Bit ADCs -> Altera Cyclone II FPGA. Spartan XC3S4000 FPGA and XC95288 CPLD (both Xilinx) <br />
* Data is collected by the [https://www.activesilicon.com/products/phoenix-cpci-camera-link-frame-grabber/ Phoenix AS-PHX-D48CL Frame grabber card] in the Computer<br />
<br />
====Port usage====<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! Device<br />
! Port<br />
|-<br />
| ARM9BoardSerialPort<br />
| Port: ARM9CHEM ;CM00006<br />
|-<br />
| ARM9BoardDiagSerialPort<br />
| Port: ARM9DIAG<br />
|-<br />
|FPGA<br />
|Port: IL000004 / 115200 (command_com_port_num<br />
|-<br />
|<br>ExcitationFilter1 <br />
|<br>FPGACommandSuffix = 1<br />
|-<br />
|ExcitationFilter2<br />
|FPGACommandSuffix = 2<br />
|-<br />
|ChromaticCompensator<br />
|FPGACommandSuffix = 3<br />
|-<br />
|TiltMotor_FPGATilt<br />
|<br />
|-<br />
|ZMotor_FPGAControlledPIMotor_250um<br />
|<br />
|-<br />
|ZADCR command<br />
|<br />
|-<br />
|<br>FlowcellFluidics1<br />
|<br />
|-<br />
|FtdiViciValve1:<br />
|Port: VICIA1 ;CM00004<br />
|-<br />
|FtdiViciValve2:<br />
|Port: VICIA2 ;CM00043<br />
|-<br />
|KloehnControllerPump<br />
|Port: KLOEHNA ;CM00001<br />
|-<br />
|<br>FlowcellFluidics2<br />
|<br />
|-<br />
|FtdiViciValve1:<br />
|Port: VICIB1 ;CM00002<br />
|-<br />
|FtdiViciValve2:<br />
|Port: VICIB2 ;CM00044<br />
|-<br />
|KloehnControllerPump<br />
|Port: KLOEHNB ;CM00003<br />
|-<br />
|<br>Laser1,Green532,Smd6000<br />
|<br>Port: IL000006<br />
|-<br />
|Laser2,Red660,Smd6000<br />
|Port: IL000007<br />
|-<br />
|<br>XMotor_MDrive_5mm<br />
|<br>Port: IL000001<br />
|-<br />
|YMotor_VixServoIH_10nm <br />
|Port: IL000002<br />
|-<br />
|<br>Barcode_Reader<br />
|<br>Port: FTE2V9ML ; COM5 (com_port_num = 4)<br />
|-<br />
|PTC<br />
|Port: COM2 (com_port_num = 1)/9600<br />
|-<br />
|rs232<br />
|Port: COM10 (offset 9)<br />
|-<br />
|PCIO board ("Test Port")<br />
|Port: PCIO (com_port_num) <br />
<br />
|-<br />
|<br>From Video:<br />
|<br />
|-<br />
|Physical Port on Computer<br />
|Port: COM1<br />
|-<br />
|HiSeq Cameras (Phoenix)<br />
|Ports: COM3 COM4<br />
|-<br />
|Virtual Connections<br />
|Ports: COM5 to COM23<br />
|-<br />
<br />
|}<br />
Scanner.ChemistryModule<br />
<br />
====Drives====<br />
Data (D:)<br />
Data (E:)<br />
DVD (F:)<br />
Removable (G:)<br />
DoNoEject (H:)<br />
<br />
=== OpenSeq ===<br />
How about making an Open Source Next Level Sequencing machine - OpenSeq.<br><br />
Maybe a bit slower and smaller.. like 1 genome per day 🙂<br><br />
Not sure a price tag of 500k euro is justified for such a machine...<br><br />
Maybe similar to the iSeq100:<br><br />
https://www.illumina.com/systems/sequencing-platforms/iseq.html</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=24751Worm Composting2018-02-14T06:32:45Z<p>Derishus: /* Worm Anatomy as it relates to Composting */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
* '''Esophagus''' : Calcium Carbonate secretion to remove excess calcium from the body, and neutralize acidic food<br />
* '''Gizzard''' : This is where the food is mashed physically by sand<br />
* '''Intestines''' : This is where enzymatic and microbial processing of the ingested foodstuff gets digested<br />
** mucus containing protein and polysaccharides<br />
*** digestive enzymes such as amylase, cellulase, protease, lipase, chitinase and urease<br />
** organic and mineral matter<br />
** amino acids <br />
** '''It's not only worms''' - microbial symbionts (bacteria, protozoa and microfungi) - also responsible for some of the digestive enzymes)<br />
* '''Anus''' : This is where the processed worm castings (waste, i.e. poop) comes out. One man's waste is another one's gold!<br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
<br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
<br />
For ''E. foetida'' reproduction, taken from [http://www.sierra-worm-compost.com/composting-worms.html Sierra worm compost]<br><br />
* Produce 3.8 cocoon per adult per week.<br />
* Each cocoon contains between 8-20 eggs, but on average only 3.3 worms will actually hatch.<br />
* Hatching occurs in about 3 weeks.<br />
* 53-76 days to sexual maturity.<br />
* 85-149 days from egg to maturity.<br />
<br />
<br><br><br />
This [https://youtu.be/KbhjqMngGz4 video] shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Science<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
* [https://animals.howstuffworks.com/animal-facts/earthworm.htm How Stuff Works, Earth Worm]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=24750Worm Composting2018-02-14T03:02:31Z<p>Derishus: /* Worm Anatomy as it relates to Composting */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
* '''Esophagus''' : Calcium Carbonate secretion to remove excess calcium from the body, and neutralize acidic food<br />
* '''Gizzard''' : This is where the food is mashed physically by sand<br />
* '''Intestines''' : This is where enzymatic and microbial processing of the ingested foodstuff gets digested<br />
** mucus containing protein and polysaccharides<br />
*** digestive enzymes such as amylase, cellulase, protease, lipase, chitinase and urease<br />
** organic and mineral matter<br />
** amino acids <br />
** microbial symbionts (bacteria, protozoa and microfungi) - also responsible for some of the digestive enzymes)<br />
* '''Anus''' : This is where the processed worm castings (waste, i.e. poop) comes out. One man's waste is another one's gold!<br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
<br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
<br />
For ''E. foetida'' reproduction, taken from [http://www.sierra-worm-compost.com/composting-worms.html Sierra worm compost]<br><br />
* Produce 3.8 cocoon per adult per week.<br />
* Each cocoon contains between 8-20 eggs, but on average only 3.3 worms will actually hatch.<br />
* Hatching occurs in about 3 weeks.<br />
* 53-76 days to sexual maturity.<br />
* 85-149 days from egg to maturity.<br />
<br />
<br><br><br />
This [https://youtu.be/KbhjqMngGz4 video] shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Science<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
* [https://animals.howstuffworks.com/animal-facts/earthworm.htm How Stuff Works, Earth Worm]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=24749Worm Composting2018-02-14T02:59:47Z<p>Derishus: /* Worm Anatomy as it relates to Composting */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
* '''Esophagus''' : Calcium Carbonate secretion to remove excess calcium from the body, and neutralize acidic food<br />
* '''Gizzard''' : This is where the food is mashed physically by sand<br />
* '''Intestines''' : This is where enzymatic and microbial processing of the ingested foodstuff gets digested<br />
** mucus containing protein and polysaccharides<br />
*** digestive enzymes such as amylase, cellulase, protease, lipase, chitinase and urease<br />
** organic and mineral matter<br />
** amino acids <br />
** microbial symbionts (bacteria, protozoa and microfungi)<br />
* '''Anus''' : This is where the processed worm castings (waste, i.e. poop) comes out. One man's waste is another one's gold!<br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
<br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
<br />
For ''E. foetida'' reproduction, taken from [http://www.sierra-worm-compost.com/composting-worms.html Sierra worm compost]<br><br />
* Produce 3.8 cocoon per adult per week.<br />
* Each cocoon contains between 8-20 eggs, but on average only 3.3 worms will actually hatch.<br />
* Hatching occurs in about 3 weeks.<br />
* 53-76 days to sexual maturity.<br />
* 85-149 days from egg to maturity.<br />
<br />
<br><br><br />
This [https://youtu.be/KbhjqMngGz4 video] shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Science<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
* [https://animals.howstuffworks.com/animal-facts/earthworm.htm How Stuff Works, Earth Worm]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=24748Worm Composting2018-02-14T02:56:38Z<p>Derishus: /* Worm Anatomy as it relates to Composting */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
* '''Esophagus''' : Calcium Carbonate secretion to remove excess calcium from the body, and neutralize acidic food<br />
* '''Gizzard''' : This is where the food is mashed physically by sand<br />
* '''Intestines''' : This is where enzymatic and microbial processing of the ingested foodstuff gets digested<br />
:: * mucus containing protein and polysaccharides<br />
:: * organic and mineral matter<br />
:: * amino acids <br />
:: * microbial symbionts (bacteria, protozoa and microfungi)<br />
* '''Anus''' : This is where the processed worm castings (waste, i.e. poop) comes out. One man's waste is another one's gold!<br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
<br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
<br />
For ''E. foetida'' reproduction, taken from [http://www.sierra-worm-compost.com/composting-worms.html Sierra worm compost]<br><br />
* Produce 3.8 cocoon per adult per week.<br />
* Each cocoon contains between 8-20 eggs, but on average only 3.3 worms will actually hatch.<br />
* Hatching occurs in about 3 weeks.<br />
* 53-76 days to sexual maturity.<br />
* 85-149 days from egg to maturity.<br />
<br />
<br><br><br />
This [https://youtu.be/KbhjqMngGz4 video] shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Science<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
* [https://animals.howstuffworks.com/animal-facts/earthworm.htm How Stuff Works, Earth Worm]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23827Worm Composting2018-02-13T05:03:05Z<p>Derishus: /* Worm Reproduction */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
* '''Esophagus''' : Calcium Carbonate secretion to remove excess calcium from the body, and neutralize acidic food<br />
* '''Gizzard''' : This is where the food is mashed physically by sand<br />
* '''Intestines''' : This is where enzymatic and microbial processing of the ingested foodstuff gets digested<br />
* '''Anus''' : This is where the processed worm castings (waste, i.e. poop) comes out. One man's waste is another one's gold!<br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
<br />
For ''E. foetida'' reproduction, taken from [http://www.sierra-worm-compost.com/composting-worms.html Sierra worm compost]<br><br />
* Produce 3.8 cocoon per adult per week.<br />
* Each cocoon contains between 8-20 eggs, but on average only 3.3 worms will actually hatch.<br />
* Hatching occurs in about 3 weeks.<br />
* 53-76 days to sexual maturity.<br />
* 85-149 days from egg to maturity.<br />
<br />
<br><br><br />
This [https://youtu.be/KbhjqMngGz4 video] shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Science<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
* [https://animals.howstuffworks.com/animal-facts/earthworm.htm How Stuff Works, Earth Worm]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23826Worm Composting2018-02-13T04:58:10Z<p>Derishus: /* What is Vermicomposting? */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
* '''Esophagus''' : Calcium Carbonate secretion to remove excess calcium from the body, and neutralize acidic food<br />
* '''Gizzard''' : This is where the food is mashed physically by sand<br />
* '''Intestines''' : This is where enzymatic and microbial processing of the ingested foodstuff gets digested<br />
* '''Anus''' : This is where the processed worm castings (waste, i.e. poop) comes out. One man's waste is another one's gold!<br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
This video shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms ([https://youtu.be/KbhjqMngGz4 video]) using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Science<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
* [https://animals.howstuffworks.com/animal-facts/earthworm.htm How Stuff Works, Earth Worm]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23825Worm Composting2018-02-13T04:50:22Z<p>Derishus: /* Links */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
This video shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms ([https://youtu.be/KbhjqMngGz4 video]) using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Science<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
* [https://animals.howstuffworks.com/animal-facts/earthworm.htm How Stuff Works, Earth Worm]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23824Worm Composting2018-02-13T04:49:13Z<p>Derishus: /* What is Vermicomposting? */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
==== Worm Reproduction ====<br />
Worm reproduction is a wonderful thing - this will allow you to share the worms with other people.<br><br><br />
This video shows the reproductive process - worms are [https://animals.howstuffworks.com/animal-facts/earthworm3.htm SIMULTANEOUS hermaphrodites], and sexual intercourse between two worms ([https://youtu.be/KbhjqMngGz4 video]) using both male and female reproductive organs will give rise to worm eggs ([https://youtu.be/EwW4FzRQpgg?t=2m34s video]).<br><br />
<br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Biology<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23823Worm Composting2018-02-13T04:32:16Z<p>Derishus: /* Worm Anatomy as it relates to Composting */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|400px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Biology<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23822Worm Composting2018-02-13T04:31:56Z<p>Derishus: /* Worm Anatomy as it relates to Composting */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|thumb|800px|Image from North Allegheny School District Earthworm Dissection Page]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
<br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Biology<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23821Worm Composting2018-02-13T04:30:02Z<p>Derishus: /* What is Vermicomposting? */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<blockquote><br />
'''Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms''' (Garg and Gupta, 2009).<br />
</blockquote><br />
<br />
==== Worm Anatomy as it relates to Composting ====<br />
[[File:Earthworm lettered key.gif|800px]]<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<br />
<br><br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Biology<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=File:Earthworm_lettered_key.gif&diff=23820File:Earthworm lettered key.gif2018-02-13T04:28:07Z<p>Derishus: https://www.northallegheny.org/Page/19944</p>
<hr />
<div>https://www.northallegheny.org/Page/19944</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23819Worm Composting2018-02-13T04:27:26Z<p>Derishus: /* Links */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<blockquote><br />
Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms (Garg and Gupta, 2009).<br />
</blockquote><br />
<br><br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Biology<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
* [https://www.northallegheny.org/Page/19944 NA SENIOR HIGH SCHOOL Earth Worm Dissection]<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23818Worm Composting2018-02-13T04:23:31Z<p>Derishus: /* Troubleshooting and Improvements */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<blockquote><br />
Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms (Garg and Gupta, 2009).<br />
</blockquote><br />
<br><br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
'''NICE TROUBLESHOOTING TABLE''' [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Biology<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23817Worm Composting2018-02-13T04:23:08Z<p>Derishus: /* Observations and Improvements */</p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<blockquote><br />
Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms (Garg and Gupta, 2009).<br />
</blockquote><br />
<br><br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Troubleshooting and Improvements ==<br />
Using composting worms (''Eisenia foetida''), there are hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
NICE TROUBLESHOOTING TABLE [http://www.sierra-worm-compost.com/worm-bin-problems.html HERE by Sierra Worm Compost].<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Biology<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishushttp://www.hackteria.org/wiki/index.php?title=Worm_Composting&diff=23816Worm Composting2018-02-13T04:06:48Z<p>Derishus: </p>
<hr />
<div>The neighborhood started charging more tax for garbage (yay!)<br> <br />
and the compost small plastic bins in the summer would sit for too long and generate tons of flies and other creatures...and smelled very bad (boo!). <br><br />
This was great motivation to try some worm composting. <br><br><br />
== What is Vermicomposting? ==<br />
=== The Biology and Chemistry ===<br />
<br />
The Biology and Chemistry in a nutshell in this short video by [https://youtu.be/2Pa1FwmKZcQ PBS and American Chemical Society].<br><br><br />
Some more details here in the [http://www.sierra-worm-compost.com/worm-biology.html Worm Biology]<br><br><br />
<blockquote><br />
Vermicomposting is a process that involves chemical, physical, and biological transformations of solid organic materials (agricultural residues of plant and animal origin) through the use of worms and microorganisms (Garg and Gupta, 2009).<br />
</blockquote><br />
<br><br />
=== Worms ===<br />
Which worms can we use?<br><br />
There are different types of worms <br><br />
Overall categorization:<br><br />
[[File:Different_worms.jpg|800px]]<br><br />
More detailed table:<br><br />
[[File:Table_of_Worms.gif|800px]]<br><br />
<br><br />
<br />
== Prototype ==<br />
=== Concept behind the Prototype ===<br />
Worm composting can be done indoors in small apartments, as well as outdoors. <br><br />
Some basics:<br />
* Compost vegetable matter, even coffee rinds! - except egg shells - BUT '''no meat, no starch''' in your compost<br />
* Good moisture<br />
* Not in direct sunlight<br />
* Good air circulation (turn it over)<br />
* Balance the worm diet and balance YOUR diet! (too much citrus, coffee rinds will acidify, etc.)<br />
<br><br />
<br />
There is a lot online on how to start one (links below) - this one aimed for these features:<br />
* One person<br />
* Balcony location<br />
* No need to touch the worms<br />
<br />
The design here assumes that the worms are smart enough to want to look for food i.e. that they are epigeics (the worms like surface soil), which will stay on the top of the composting bin.<br />
<br><br><br />
<br />
=== Materials and Tools ===<br />
* Plastic garbage bin<br />
* Plastic receptacle<br />
* Plastic receiving tray<br />
* Metal chickenwire / mesh<br />
* Metal support rods<br />
* Cutter for plastic<br />
* Cutter for Wires<br />
* Drill (to make airholes)<br />
* A ziptie for a handle<br />
* Salad turners for "tossing the compost" for aeration<br />
<br><br />
<br />
=== Worms ===<br />
Can you dig up worms yourself in the garden and do this?<br> <br />
The answer is no, for this type of design. See above for the types of worms.<br><br />
Otherwise, look for suppliers in the neighborhood.<br><br />
<br />
<br><br />
<br />
=== Build it ===<br />
{|class="wikitable" style="vertical-align:top;"<br />
|[[File:wormcompost_start.jpeg|x250px]]<br />
|<br />
Choose a bin.<br><br />
This one was chosen because it was lying around<br><br />
but also because there was a pedal to open the lid -<br> <br />
no need to juggle the organic waste, and try to open the lid of the compost bin.<br><br />
<br />
|-<br />
|[[File:wormcompost_receptacle.jpeg|x250px]]<br />
|<br />
Take an old tupperware, something that fits into the compost bin. <br><br />
Cut a window big enough so that the receptacle can easily be taken out.<br><br />
Next time around though, the window should be higher off the ground to allow for some brown water to collect,<br><br />
so that another tray is not necessary.<br><br />
<br />
|-<br />
|[[File:wormcompost_supportgrid.jpeg|x250px]]<br />
|<br />
Some height above the receptacle, 2 aluminum rods were inserted to support the chicken wire.<br><br />
Surprisingly, this is sufficient - even though it is super holey, to support the compost.<br><br />
Hardly any worms drop down, nor are there huge chunks of random things that fall down.<br><br />
Here, you can also see the drill holes for aeration.<br><br />
<br />
|-<br />
|[[File:wormcompost_firstlayer.jpeg|x250px]]<br />
|<br />
For initial support, layer some cardboard.<br><br />
Worms eat cardboard!<br> <br />
<br />
|-<br />
|[[File:wormcompost_firstorganics.jpeg|x250px]]<br />
|<br />
Then layer some food.<br><br />
People mix with dirt...<br><br />
<br />
|-<br />
|[[File:wormcompost_addpaper.jpeg|x250px]]<br />
|<br />
Or just some wet newspaper.<br><br />
<br />
|-<br />
|[[File:wormcompost_3months.jpeg|x250px]]<br />
|<br />
No fruitflies no maggots, less garbage no odors and no trips to the local organic garbage bin. <br><br />
The worm numbers are increasing.<br><br />
The sound of them moving around at night is mesmerizing.<br><br />
<br />
|-<br />
|[[File:wormcompost_2years.JPG|x250px]]<br />
|<br />
After 2 years and a move it is going strong!<br><br />
The main issue is the brown water - ideas to improve the design, see below.<br><br />
|}<br />
<br><br><br />
== Repeat of What not to Compost ==<br />
What not to compost at home:<br><br />
* Dairy products<br />
* Meats<br />
* Oils, fats<br />
* Pet waste (contains harmful pathogens - unless processed in heat compost)<br />
* Sugars (attracts ants)<br />
<br><br />
In effect, a good vegetarian mix + also, coffee, tea, plants, paper (cellulose).<br><br />
Rotten food is welcome. <br><br><br />
Onions, garlic or citrus (in small quantities), not too spicy also.<br><br><br />
Chop them up for easy eating.<br><br><br />
Feed them, and see how it goes.<br><br><br />
<br />
== Observations and Improvements ==<br />
Surprisingly, and luckily, hardly any worms try to escape from the bottom. There was never a need to "separate" the worms from the compost, which most articles suggested to do. But here are some things to do differently than this version.<br><br />
* Need a bigger bin!<br />
* Make the "bottom window" to take out the compost a bit higher off the ground, to catch the brown water, especially for indoor composting.<br />
<br><br><br />
<br />
== Links ==<br />
=== Other articles ===<br />
* [https://growingwildceeds.wordpress.com/2012/03/03/diy-indoor-vermicomposting/ DIY indoor vermicomposting]<br />
* [https://www.redwormcomposting.com/quick-facts-about-worm-composting/ Quick Facts about worm composting]<br />
* [http://working-worms.com/how-to-make-your-own-worm-farm/ 3-tier system]<br />
* [https://www.tenthacrefarm.com/2014/02/fruit-flies-worm-bin/ preventing fruit flies!] very useful tips toward the end of the article<br />
* [https://www.imperfectlyhappy.com/troubleshooting-your-compost-3/ Troubleshooting your Compost]<br />
Worm Biology<br><br />
* [http://www.printablediagram.com/printable-earthworm-diagrams/ Worm anatomy diagrams]<br />
* [http://www.sierra-worm-compost.com/ Sierra worm compost website] - great summary including more scientific things<br />
<br />
=== Worm Sources ===<br />
FR<br><br />
* [http://www.verslaterre.fr/ vers la terre] <br />
<br />
JP<br><br />
* [http://www.nokei.co.jp/mmz/index.html 株式会社 農業経営研究所] <br />
* [http://www.kowas.co.jp/mimizu/start.html 光和商事株式会社] <br />
* [http://www.sef.or.jp/earthworm/earthworm_kyozaiitiran.htm#kounyuhoho 公益財団法人 科学教育研究会 研究所] <br />
<br />
[[Category:Growing Things]]<br />
[[Category:Wetware]]<br />
[[Category:Environment]]</div>Derishus