Difference between revisions of "DIY Micro Dispensing and Bio Printing"
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== Robotic Stage == | == Robotic Stage == | ||
− | [[File: | + | [[File:MicroManipulatorSetup2.jpg|500px|none|thumb|HackStage V1]] |
Small and precise mechanical manipulator made from recycled material and using open hard and software.<br> | Small and precise mechanical manipulator made from recycled material and using open hard and software.<br> | ||
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Basic principle:<br> | Basic principle:<br> | ||
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- Two CD / DVD drives with stepper motor mounted cross wise<br> | - Two CD / DVD drives with stepper motor mounted cross wise<br> | ||
- Both drives are mounted fix, one is the object carrier the other is the manipulator<br> | - Both drives are mounted fix, one is the object carrier the other is the manipulator<br> | ||
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- G-Code may be generated with InkScape and G-Code plugin<br> | - G-Code may be generated with InkScape and G-Code plugin<br> | ||
- Controlled by ReplicatorG using G-Code<br> | - Controlled by ReplicatorG using G-Code<br> | ||
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+ | [[File:LiquidPrinterVideo.jpg|none|300px|]] | ||
+ | Printer in action video at: http://www.youtube.com/watch?v=E39qfKR-5s4 | ||
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+ | [[File:PrinterPrecision.jpg|none|300px|]] | ||
+ | Calibration Measurement. With a micro stepping motor driver a | ||
+ | precision of less than 60 um can be achieved. | ||
[[File:ReplicatorGScreen.jpg|none|200px|]] | [[File:ReplicatorGScreen.jpg|none|200px|]] | ||
+ | Screenshot with ReplicatorG and WebCam image | ||
+ | [[File:PrintedSample2.jpg|none|200px|]] | ||
+ | "Printed" sample | ||
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[[File:MicroDropEPF.jpg|none|200px|]] | [[File:MicroDropEPF.jpg|none|200px|]] | ||
Comercial Dispensing Robot from MicroDrop at Ecole Polytechnique Federal Lausanne (EPFL) | Comercial Dispensing Robot from MicroDrop at Ecole Polytechnique Federal Lausanne (EPFL) | ||
+ | <br> | ||
+ | Can be used for:<br> | ||
+ | - Laser cutting masks or light materials using a DVD-laser-diode<br> | ||
+ | Here an istruction on how to make a laser cutter: | ||
+ | [[DIY Micro Laser Cutter]]<br> | ||
+ | - Manipulate organisms or material<br> | ||
+ | - Stage for microscopy<br> | ||
+ | - Tissue engineering<br> | ||
+ | (yeah yeah we print some fungi :-)<br> | ||
+ | - Mini extruder<br> | ||
+ | - SMD pick and place / stencil cutter<br> | ||
+ | <br> | ||
+ | [[File:MicroManipulator.png|none|300px|]] | ||
+ | Some link to sources:<br> | ||
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RepRap G-Code: http://reprap.org/wiki/G-code#T:_Select_Tool<br> | RepRap G-Code: http://reprap.org/wiki/G-code#T:_Select_Tool<br> | ||
Teacup Firmware: http://reprap.org/wiki/Teacup_Firmware<br> | Teacup Firmware: http://reprap.org/wiki/Teacup_Firmware<br> | ||
Pololu Motor Driver: http://www.pololu.com/catalog/product/1201<br> | Pololu Motor Driver: http://www.pololu.com/catalog/product/1201<br> | ||
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InkScape to G-Code: http://wiki.linuxcnc.org/cgi-bin/emcinfo.pl?InkscapeHowto<br> | InkScape to G-Code: http://wiki.linuxcnc.org/cgi-bin/emcinfo.pl?InkscapeHowto<br> | ||
− | + | ReplicatorG Printing Program http://replicat.org/ | |
<br> | <br> | ||
+ | == Micro-Dispensing == | ||
+ | [[File:PrintingTip.JPG|300px|none|thumb|Printing tip mad from a glas tube]] | ||
− | + | [[File:FirstTip.jpg|none|200px|]] | |
+ | First try printing tip made from glas tubing | ||
+ | <br> | ||
+ | [[File:DIYPiezoNozzle.jpg|none|200px|]] | ||
+ | First try DIY piezo nozzle (does not work properly) | ||
+ | <br> | ||
+ | [[File:DIYTipMechanism.jpg|none|200px|]] | ||
+ | Tip holding mechanism | ||
== Printing medias == | == Printing medias == | ||
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[[File:HackteriaPrintingMedia.jpg|300px|none|thumb|First approaches during HackteriaLab 2011]] | [[File:HackteriaPrintingMedia.jpg|300px|none|thumb|First approaches during HackteriaLab 2011]] | ||
+ | [[File:FunghiOnPoly.jpg|none|200px|]] | ||
+ | Funghi growing on polyvinyl with LB-Medium | ||
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+ | [[File:PrintedSample.JPG|300px|none|thumb|Finally urs got it done, super awesome]] | ||
== Related Links == | == Related Links == | ||
+ | DIY Laser Cutter: http://www.flickr.com/photos/imajilon/2210335514/in/photostream/<br> | ||
+ | RepRap Laser Sintering: http://builders.reprap.org/2010/08/selective-laser-sintering-part-8.html<br> | ||
+ | RepRap Tissue Engineering: http://forums.reprap.org/list.php?194<br> | ||
+ | DIY Piezo Printhead http://reprap.org/wiki/Scratchbuilt_Piezo_Printhead<br> | ||
+ | Comercial dispensing robots and piezo nozzles http://www.microdrop.de/<br> | ||
+ | Growing Body Parts in the Lab Becomes Reality http://www.worldhealth.net/news/growing_body_parts_in_the_lab_becomes_re/<br> | ||
+ | Controlling FIAlab Devices for Microfluidics http://88proof.com/synthetic_biology/blog/archives/407<br> | ||
+ | Are Mushrooms The New Plastic http://www.ted.com/talks/eben_bayer_are_mushrooms_the_new_plastic.html<br> | ||
+ | easy-to-use atomic force microscopes (AFM) and scanning tunneling microscopes (STM) http://www.nanosurf.com/<br> | ||
+ | Hollow cantilever for liquid dispensing http://www.cytosurge.com/<br> | ||
+ | General cell printing using an ink-jet http://www.sciencedaily.com/releases/2012/03/120316112648.htm<br> | ||
+ | <br> |
Latest revision as of 12:26, 5 July 2012
Contents
Robotics, Pipettes Medias and more for Micro Dispensing and Bio Printing
Automatic liquid handling or microdispensing of small liquid amounts is useful in the production of biochips, Lab-on-a-Chip (LOC), biosensors and for tissue engineering. Crucial elements are: - Precise manipulation / XY(Z) robotics - Micro-dispensing and other formes liquid manipulation - Printing medias
Robotic Stage
Small and precise mechanical manipulator made from recycled material and using open hard and software.
Basic principle:
- Two CD / DVD drives with stepper motor mounted cross wise
- Both drives are mounted fix, one is the object carrier the other is the manipulator
- Two stepper motor drivers (ex. pololu 4988) with micro stepping
- RepRap driver-software teacup on an arduino board
- G-Code may be generated with InkScape and G-Code plugin
- Controlled by ReplicatorG using G-Code
Printer in action video at: http://www.youtube.com/watch?v=E39qfKR-5s4
Calibration Measurement. With a micro stepping motor driver a precision of less than 60 um can be achieved.
Screenshot with ReplicatorG and WebCam image
"Printed" sample
Comercial Dispensing Robot from MicroDrop at Ecole Polytechnique Federal Lausanne (EPFL)
Can be used for:
- Laser cutting masks or light materials using a DVD-laser-diode
Here an istruction on how to make a laser cutter:
DIY Micro Laser Cutter
- Manipulate organisms or material
- Stage for microscopy
- Tissue engineering
(yeah yeah we print some fungi :-)
- Mini extruder
- SMD pick and place / stencil cutter
Some link to sources:
RepRap G-Code: http://reprap.org/wiki/G-code#T:_Select_Tool
Teacup Firmware: http://reprap.org/wiki/Teacup_Firmware
Pololu Motor Driver: http://www.pololu.com/catalog/product/1201
InkScape to G-Code: http://wiki.linuxcnc.org/cgi-bin/emcinfo.pl?InkscapeHowto
ReplicatorG Printing Program http://replicat.org/
Micro-Dispensing
First try printing tip made from glas tubing
First try DIY piezo nozzle (does not work properly)
Tip holding mechanism
Printing medias
Funghi growing on polyvinyl with LB-Medium
Related Links
DIY Laser Cutter: http://www.flickr.com/photos/imajilon/2210335514/in/photostream/
RepRap Laser Sintering: http://builders.reprap.org/2010/08/selective-laser-sintering-part-8.html
RepRap Tissue Engineering: http://forums.reprap.org/list.php?194
DIY Piezo Printhead http://reprap.org/wiki/Scratchbuilt_Piezo_Printhead
Comercial dispensing robots and piezo nozzles http://www.microdrop.de/
Growing Body Parts in the Lab Becomes Reality http://www.worldhealth.net/news/growing_body_parts_in_the_lab_becomes_re/
Controlling FIAlab Devices for Microfluidics http://88proof.com/synthetic_biology/blog/archives/407
Are Mushrooms The New Plastic http://www.ted.com/talks/eben_bayer_are_mushrooms_the_new_plastic.html
easy-to-use atomic force microscopes (AFM) and scanning tunneling microscopes (STM) http://www.nanosurf.com/
Hollow cantilever for liquid dispensing http://www.cytosurge.com/
General cell printing using an ink-jet http://www.sciencedaily.com/releases/2012/03/120316112648.htm