Difference between revisions of "Micro-electro-physiology @ HAIP, Ljubljana"
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− | ''Analog to Digital Converter (ADC)'' | + | '''Analog to Digital Converter (ADC)''' |
Using the arduino we can measure analog voltages using the 6 Inputs ADC. The analog voltage between 0 and 5 V is converted into a 10bit digital number, from 0 to 1023. | Using the arduino we can measure analog voltages using the 6 Inputs ADC. The analog voltage between 0 and 5 V is converted into a 10bit digital number, from 0 to 1023. | ||
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If the potentials we want to measure are mostly changes/modulations in voltages, we can also use the analog-to-digital converter from the sound card. Although that means we cannot measure static voltages, but only fast changes changes, frequencies above ~ 1hz. The sound card is a very fast and precise ADC (single if mono, or double for stereo), sampling at 44khz or up to 96khz and 24 bit resolution. | If the potentials we want to measure are mostly changes/modulations in voltages, we can also use the analog-to-digital converter from the sound card. Although that means we cannot measure static voltages, but only fast changes changes, frequencies above ~ 1hz. The sound card is a very fast and precise ADC (single if mono, or double for stereo), sampling at 44khz or up to 96khz and 24 bit resolution. | ||
− | ''Amplification'' | + | '''Amplification''' |
If the signals are to low, such as µV in the example of potentials in plants, we need to amplify the signal and make sure we do not have too much noise or other garbage in our signals. [http://focus.ti.com/docs/prod/folders/print/ina118.html INA118] is a high end instrumental amplifier, with low noise. But maybe cheaper amp might do the trick aswell, such as the [http://www.national.com/mpf/LM/LM386.html LM386] | If the signals are to low, such as µV in the example of potentials in plants, we need to amplify the signal and make sure we do not have too much noise or other garbage in our signals. [http://focus.ti.com/docs/prod/folders/print/ina118.html INA118] is a high end instrumental amplifier, with low noise. But maybe cheaper amp might do the trick aswell, such as the [http://www.national.com/mpf/LM/LM386.html LM386] | ||
− | ''Multiplexing'' | + | '''Multiplexing''' |
If we want to measure more inputs we can multiplex them. meaning we measure one input at the time using a chip (4051), which got 8 inputs which are then routed to one single input on the arduino. | If we want to measure more inputs we can multiplex them. meaning we measure one input at the time using a chip (4051), which got 8 inputs which are then routed to one single input on the arduino. |
Revision as of 13:01, 29 November 2010
Contents
Background Info
Electrochemistry of Plant Life
Video of extracellular measurement of action potential of the venus flytrap
Protocols
On Measuring
Analog to Digital Converter (ADC)
Using the arduino we can measure analog voltages using the 6 Inputs ADC. The analog voltage between 0 and 5 V is converted into a 10bit digital number, from 0 to 1023.
If the potentials we want to measure are mostly changes/modulations in voltages, we can also use the analog-to-digital converter from the sound card. Although that means we cannot measure static voltages, but only fast changes changes, frequencies above ~ 1hz. The sound card is a very fast and precise ADC (single if mono, or double for stereo), sampling at 44khz or up to 96khz and 24 bit resolution.
Amplification
If the signals are to low, such as µV in the example of potentials in plants, we need to amplify the signal and make sure we do not have too much noise or other garbage in our signals. INA118 is a high end instrumental amplifier, with low noise. But maybe cheaper amp might do the trick aswell, such as the LM386
Multiplexing
If we want to measure more inputs we can multiplex them. meaning we measure one input at the time using a chip (4051), which got 8 inputs which are then routed to one single input on the arduino. read more about using the 4051 multiplexer/demultiplexer
DIY Glass Pipette Electrodes
Micro Electrode Arrays (MEA)
DIY Microscopy
To be able to observe the pipette we can build our own hacked digital microscopes, using cheap webcams, surveillance video cams or the PS3 eye. see more on the hackteria page.
Slime Mold
paper about growing slime mold as a map of japan
Plant S.M.E.L.A
Setup
Experiments
Venus Fly Trap
Slime Mold
Patches for measuring
Participants
Marc Dusseiller, dusjagr labs
Špela Petrič,
Andrej Meglič,
rough schedule
Wednesday
- Introduction
- Experiments in manufacturing DIY electrodes
- intro into DIY microscopy
- using audio and pd for data measurement
- using arduino and pd the measure/visualize data
- start fungi cultures and preparations of plants
- Setup of measurement setup
Thursday
- Preparation of amplifiers and multiplexers
- Setup of measurement setup
- Experiments with plant recordings
Friday
- Wrap-up Workshop: Introduction into all techniques developed
- Preparations of individual pages for a publication using the diffusion layout.
Material for the Book
Example of book about DIY microscopy for Kids (german only)
Interesting Links
http://ediacaran.mech.northwestern.edu/neuromech/index.php/Main_Page