Actinomycetes Tournament: Open antibiotics discovery

From Hackteria Wiki
Revision as of 18:21, 17 October 2013 by Denisa kera (talk | contribs)
Jump to: navigation, search

Picture: Antibiotic Producing Actinomycetes By adonofrio (

We want to screen soil bacteria, such as Actinomyces, Streptomyces and Micromonospora for antibiotic properties. It is a global citizen science initiative started by DIYbio Waag, which uses game elements (collecting and tournaments) to support open drug discovery of antibiotics. We will isolate actinomycetes from soil samples around the world and screen with Micrococcus luteus or similar microbe to determine their antibiotic properties. Images of inoculated plates will be shared online and based on their strengths (zones of growth inhibition) they will become part of a collection or initiate tournaments between the most successful examples. We will sequence the most powerful ones. We expect cooperation with various DIYbio organizations, microbiology departments and science enthusiasts.

This is a wiki for documentation, sharing links and defining the protocol


1. Improve, standardize and simplify the protocol [1] and its different phases: diluting a soil sample, inoculation on various agar media, incubate for a specified time, cross streaks with non pathogenic bacteria, optimal temperature for incubation, examining growth inhibition, drying or amplifying with various OSHW tools and sending to our potential scientific partner for sequencing.
2. Gamification of agar plates: growth inhibition circles in the plates will define various "collections" and the most powerful ones will take part in tournaments. Every participant will have a page with his collection and the most powerful strains will be featured on the main page. All data will be shared under CC for open science with database of GPS data and pictures of the sites where the soil samples were collected and soil bank.


You will need

We will try to make this list modular, so that you can do each part independently.

Equipment for

Consumable materials