Difference between revisions of "The Art of Hunting"

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== Baermannn funnels for nematodes ==
  
Baermannn funnels for nematodes:
 
 
Collect soil samples from just about any location.
 
Collect soil samples from just about any location.
 
Construct Baermann funnels using plastic funnel with top diamter of approximately 8cm. Attach standard aquarium silicone hose to the bottom of the funnel and clamp shut. Mount funnel on stand so that it remains vertical with the hose downwards. Create packet of soil sample by placing a small amount inside filter paper or strong kitchen towel and wrap around to enclose.
 
Construct Baermann funnels using plastic funnel with top diamter of approximately 8cm. Attach standard aquarium silicone hose to the bottom of the funnel and clamp shut. Mount funnel on stand so that it remains vertical with the hose downwards. Create packet of soil sample by placing a small amount inside filter paper or strong kitchen towel and wrap around to enclose.
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Open clamp to allow a few drops of water to collect in a petri dish or similar.
 
Open clamp to allow a few drops of water to collect in a petri dish or similar.
  
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=== Coconut Baermann Funnel ===
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There is also a Coconut Bearmann Funnel, Jugaad Staaayyle, developed in [http://hackteria.org/?p=1134 Jaaga's Hackteria Distillery].
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[[File:coconut_funnel.jpg|320px|none]]
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=== Tullgren Funnel ===
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By adding a light bulb/heat on top it could be highly accellerated:
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[[File:tullgren_apparatus.png|320px|none]]
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Here is a nice instruction, taken from [http://unesdoc.unesco.org/images/0010/001023/102321eb.pdf “Low Cost Equipment for science and technology education” UNESCO, 1985]
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[[File:tullgren_apparatus.pdf]]
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[[File:Tullgren1.jpg|200px|none|thumb|DIY Tullfren Funnel]]
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[[File:Tullgren2.jpg|200px|none|thumb|DIY Tullfren Funnel form PET bottle]]
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=== Simple Funnel Setup ===
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[[File:FunnelBaloon1.jpg|200px|none|thumb|Funnel from a pet bottle]]
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[[File:FunnelBaloon2.jpg|200px|none|thumb|add a baloon and a clap to collect the organism]]
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== Moss samples for tardigrades ==
  
Moss samples for tardigrades:
 
 
Collect sun-dried moss cushions from white rocks, from natural stone walls and from terracotta roof tiles. Many tardigrades prefer calcitic stones as they need some calcite to build up their stiletto teeth. Mosses from forests are less appropriate – many tardigrades prefer mosses which become completely dry every few days and this is not the case with forest mosses. You will come across tardigrades from time to time also when screening freshwater samples from ponds. But as a rule the population density there will be much lower than in mosses. Avoid smelly and permanently damp mosses. Water bears like mosses which are free of bacteria and fungi. As a consequence it is advisable to store the collected mosses in a way that they can dry completely, e.g. you might expose your finds some time to direct sunlight or keep them in paper bags at a dry place.
 
Collect sun-dried moss cushions from white rocks, from natural stone walls and from terracotta roof tiles. Many tardigrades prefer calcitic stones as they need some calcite to build up their stiletto teeth. Mosses from forests are less appropriate – many tardigrades prefer mosses which become completely dry every few days and this is not the case with forest mosses. You will come across tardigrades from time to time also when screening freshwater samples from ponds. But as a rule the population density there will be much lower than in mosses. Avoid smelly and permanently damp mosses. Water bears like mosses which are free of bacteria and fungi. As a consequence it is advisable to store the collected mosses in a way that they can dry completely, e.g. you might expose your finds some time to direct sunlight or keep them in paper bags at a dry place.
  
 
by Andy Gracie, http://hackteria.org/?p=143
 
by Andy Gracie, http://hackteria.org/?p=143
  
Links:
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http://oldblockwriter.blogspot.com/2011_11_01_archive.html
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=== Links ===
http://en.wikipedia.org/wiki/Tullgren_funnel
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http://oldblockwriter.blogspot.com/2011/11/hunting-elusive-tardigrade.html<br>
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http://en.wikipedia.org/wiki/Tullgren_funnel<br>
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http://hackteria.org/?p=1061<br>
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== Plankton Nets ==
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===How to make them===
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[[File:plankton-net-ETE-62.jpg| 600px]]
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=== Materials ===
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* steel band/ring and screws, ca 15 - 30 cm diameter
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* aluminum soft wire
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* panty hose aka strumpf
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* urine sample container
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* hot glue
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===Examples===
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Here are some examples from the nets we made for our recent Cave µ-Safari
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[[File:planton_nets_various.jpg|480px]]
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Checking the different mesh of carious clothes and tissues. 1, small bags, 2. silk screen cloth, 3. stockings brown (mercator), 4. stockings black (muller), 5. magnification of microscpe (HTC desire screen).
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[[File:various_mesh.jpg|480px]]
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[[File:doms_checks_planktonNet.jpg|480px]]
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[[File:testing_plankton_nets.jpg|480px]]
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===Links===
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http://oldblockwriter.blogspot.com/2011/12/plankton-net-for-collecting-snall.html

Latest revision as of 13:57, 6 September 2013

Baermannn funnels for nematodes

Collect soil samples from just about any location. Construct Baermann funnels using plastic funnel with top diamter of approximately 8cm. Attach standard aquarium silicone hose to the bottom of the funnel and clamp shut. Mount funnel on stand so that it remains vertical with the hose downwards. Create packet of soil sample by placing a small amount inside filter paper or strong kitchen towel and wrap around to enclose. Place the packet in the Baermann funnel, cover with distilled water and leave for 24 – 48 hours. Open clamp to allow a few drops of water to collect in a petri dish or similar.

Coconut Baermann Funnel

There is also a Coconut Bearmann Funnel, Jugaad Staaayyle, developed in Jaaga's Hackteria Distillery.

Coconut funnel.jpg

Tullgren Funnel

By adding a light bulb/heat on top it could be highly accellerated:

Tullgren apparatus.png

Here is a nice instruction, taken from “Low Cost Equipment for science and technology education” UNESCO, 1985

File:Tullgren apparatus.pdf

DIY Tullfren Funnel
DIY Tullfren Funnel form PET bottle


Simple Funnel Setup

Funnel from a pet bottle
add a baloon and a clap to collect the organism

Moss samples for tardigrades

Collect sun-dried moss cushions from white rocks, from natural stone walls and from terracotta roof tiles. Many tardigrades prefer calcitic stones as they need some calcite to build up their stiletto teeth. Mosses from forests are less appropriate – many tardigrades prefer mosses which become completely dry every few days and this is not the case with forest mosses. You will come across tardigrades from time to time also when screening freshwater samples from ponds. But as a rule the population density there will be much lower than in mosses. Avoid smelly and permanently damp mosses. Water bears like mosses which are free of bacteria and fungi. As a consequence it is advisable to store the collected mosses in a way that they can dry completely, e.g. you might expose your finds some time to direct sunlight or keep them in paper bags at a dry place.

by Andy Gracie, http://hackteria.org/?p=143


Links

http://oldblockwriter.blogspot.com/2011/11/hunting-elusive-tardigrade.html
http://en.wikipedia.org/wiki/Tullgren_funnel
http://hackteria.org/?p=1061

Plankton Nets

How to make them

Plankton-net-ETE-62.jpg

Materials

  • steel band/ring and screws, ca 15 - 30 cm diameter
  • aluminum soft wire
  • panty hose aka strumpf
  • urine sample container
  • hot glue

Examples

Here are some examples from the nets we made for our recent Cave µ-Safari

Planton nets various.jpg

Checking the different mesh of carious clothes and tissues. 1, small bags, 2. silk screen cloth, 3. stockings brown (mercator), 4. stockings black (muller), 5. magnification of microscpe (HTC desire screen).

Various mesh.jpg

Doms checks planktonNet.jpg

Testing plankton nets.jpg

Links

http://oldblockwriter.blogspot.com/2011/12/plankton-net-for-collecting-snall.html